Physics of lumen growth

Dasgupta, Sabyasachi; Gupta, Kapish; Zhang, Yue; Viasnoff, Virgile; Prost, Jacques

doi:10.1073/pnas.1722154115

Cited by 74 publications

(59 citation statements)

References 21 publications

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“…This description is used to effectively capture cell-volume ( v ) changes due to compressibility of intracellular cytoplasmic fluid, cortical network present within the cell as well as passive exchange of material with the environment. We did not explicitly model any osmotic or active transport due to fluxes of solutes or fluid 41 . The basal side of the tissue was assumed to be fixed.…”

Section: Resultsmentioning

confidence: 99%

Syncytial germline architecture is actively maintained by contraction of an internal actomyosin corset

et al. 2018

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Syncytial architecture is an evolutionarily-conserved feature of the germline of many species and plays a crucial role in their fertility. However, the mechanism supporting syncytial organization is largely unknown. Here, we identify a corset-like actomyosin structure within the syncytial germline of Caenorhabditis elegans, surrounding the common rachis. Using laser microsurgery, we demonstrate that actomyosin contractility within this structure generates tension both in the plane of the rachis surface and perpendicular to it, opposing membrane tension. Genetic and pharmacological perturbations, as well as mathematical modeling, reveal a balance of forces within the gonad and show how changing the tension within the actomyosin corset impinges on syncytial germline structure, leading, in extreme cases, to sterility. Thus, our work highlights a unique tissue-level cytoskeletal structure, and explains the critical role of actomyosin contractility in the preservation of a functional germline.

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Section: Resultsmentioning

confidence: 99%

Syncytial germline architecture is actively maintained by contraction of an internal actomyosin corset

et al. 2018

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“…In addition to the sinusoidal network, liver tissue comprises a second network, not considered here, the bile canaliculi network , which transports bile fluid containing digestive enzymes [ 57 , 58 ] (for recent digital reconstructions see [ 13 , 14 , 22 ]). Like the sinusoidal network, the bile canaliculi network spans across the entire liver lobule and contacts every single hepatocyte.…”

Section: Discussionmentioning

confidence: 99%

Resilience of three-dimensional sinusoidal networks in liver tissue

et al. 2020

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Can three-dimensional, microvasculature networks still ensure blood supply if individual links fail? We address this question in the sinusoidal network, a plexus-like microvasculature network, which transports nutrient-rich blood to every hepatocyte in liver tissue, by building on recent advances in high-resolution imaging and digital reconstruction of adult mice liver tissue. We find that the topology of the three-dimensional sinusoidal network reflects its two design requirements of a space-filling network that connects all hepatocytes, while using shortest transport routes: sinusoidal networks are sub-graphs of the Delaunay graph of their set of branching points, and also contain the corresponding minimum spanning tree, both to good approximation. To overcome the spatial limitations of experimental samples and generate arbitrarily-sized networks, we developed a network generation algorithm that reproduces the statistical features of 0.3-mm-sized samples of sinusoidal networks, using multiobjective optimization for node degree and edge length distribution. Nematic order in these simulated networks implies anisotropic transport properties, characterized by an empirical linear relation between a nematic order parameter and the anisotropy of the permeability tensor. Under the assumption that all sinusoid tubes have a constant and equal flow resistance, we predict that the distribution of currents in the network is very inhomogeneous, with a small number of edges carrying a substantial part of the flow-a feature known for hierarchical networks, but unexpected for plexus-like networks. We quantify network resilience in terms of a permeability-at-risk, i.e., permeability as function of the fraction of removed edges. We find that sinusoidal networks are resilient to random removal of edges, but vulnerable to the removal of high-current edges. Our findings suggest the existence of a mechanism counteracting flow inhomogeneity to balance metabolic load on the liver.

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“…To date, a number of theoretical frameworks have been proposed to model lumen growth, focusing either on the early phase of lumen nucleation (see Glossary, Box 1) and coalescence (Dasgupta et al, 2018;Duclut et al, 2019;Dumortier et al, 2019), or on the later phase when the singly resolved lumen undergoes size oscillations (Ruiz-Herrero et al, 2017). A general assumption of these models is that lumen growth is governed by water intake resulting from inward ion transport (for example by sodium-potassium pumps).…”

Section: Lumen Formationmentioning

confidence: 99%

Integration of luminal pressure and signalling in tissue self-organization

Chan

Hiiragi

2020

Development

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Many developmental processes involve the emergence of intercellular fluid-filled lumina. This process of luminogenesis results in a build up of hydrostatic pressure and signalling molecules in the lumen. However, the potential roles of lumina in cellular functions, tissue morphogenesis and patterning have yet to be fully explored. In this Review, we discuss recent findings that describe how pressurized fluid expansion can provide both mechanical and biochemical cues to influence cell proliferation, migration and differentiation. We also review emerging techniques that allow for precise quantification of fluid pressure in vivo and in situ. Finally, we discuss the intricate interplay between luminogenesis, tissue mechanics and signalling, which provide a new dimension for understanding the principles governing tissue self-organization in embryonic development.

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Physics of lumen growth

Cited by 74 publications

References 21 publications

Syncytial germline architecture is actively maintained by contraction of an internal actomyosin corset

Syncytial germline architecture is actively maintained by contraction of an internal actomyosin corset

Resilience of three-dimensional sinusoidal networks in liver tissue

Integration of luminal pressure and signalling in tissue self-organization

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