Physiologic ischaemic training induces endothelial progenitor cell mobilization and myocardial angiogenesis via endothelial nitric oxide synthase related pathway in rabbits

Abstract

PIT improved EPC mobilization and contributed to compensatory neovascularization via eNOS-related pathway. These results might support the future development of strategies for therapeutic neovascularization.

Remote physiological ischemic training promotes coronary angiogenesis via molecular and cellular mobilization after myocardial ischemia

“…Positive relationships between EPCs and capillary density, and capillary density and CCBF as well as CCBF/CBF were consistent with previous studies . Our additional findings of the nonlinear relationships showing between capillary density and CCBF, as well as capillary density and CCBF/CBF, however, suggest that there might exist a dose‐dependent model and that absolute values of capillary density above the threshold may further improve CCBF.…”

“…Here, a promising intervention, PIT, which comprises brief episodes of reversible ischemia on remote normal limbs, is introduced to tackle such problem. The optimal training protocol revealed by our colleagues demonstrated its beneficial effects on capillary density, CCBF, and CCBF/CBF in a controllable myocardial ischemic animal model. To determine its potential underlying molecular and cellular mechanism, various inhibitors were applied and the corresponding four experimental groups, namely VEGF‐, NO‐, VEGF‐/NO‐, and EPC‐, were added.…”

“…This procedure started 1 week after the balloon implantation and was repeated twice a day for 4 weeks. To confirm that the balloon was in the correct position during the training period, ECG examination was performed twice a week …”

“…MI rabbit models were randomized into eight groups . Assignment of MI rabbit models was based on a computer‐generated randomization list and allocation concealed by consecutively numbered, sealed opaque envelopes.…”

“…The cuff inflation was conducted for three times with 5 minutes of reperfusion in between. This training was continuously performed for a period of 4 weeks …”