“…To identify ANME and syntrophic SRB lineages in seeps, we constructed phylogenomic trees of ANME and syntrophic SRB based on archaeal and bacterial single-copy marker genes, respectively. We collected 41 previously published ANME genomes [25][26][27][28][29][30][31][32][33] , including all of the currently described subclades (ANME-1, ANME-2a, ANME-2b, ANME-2c, ANME-2d, and ANME-3), as well as 60 SRB genomes, including syntrophic (HotSeep-1, Seep-SRB2, Seep-SRB1a, and Seep-SRB1g) and non-syntrophic SRB [34][35][36][37] . According to the phylogenomic distance, we found 81 ANME genomes spanning five subclades including ANME-1 (n = 38), ANME-2a (n = 16), ANME-2b (n = 1), ANME-2c (n = 24), and ANME-3 (n = 2) (Fig.…”