Physiological adaptation to low temperatures of strains of Rhizobium leguminosarum bv. viciae associated with Lathyrus spp.

Abstract

Strains of Rhizobium leguminosarum bv. viciae, isolated from the legume species Lathyrus japonicus and Lathyrus pratensis in northern Quebec (Canada), showed different capacities for growing at low temperature. In the present study, we investigated some mechanisms related to cold adaptation. Two cold-adapted strains (psychrotrophs) were compared to a poorly adapted strain and to a cold-sensitive strain (reference strain) for freezing survival, protein induction and fatty acid composition under low temperature. Following cold shocks (25³C to 10, 5 and 0³C), a common 6.1-kDa CSP (cold shock protein) was induced in all strains, but the total number of CSPs synthesized at 0³C was higher in cold-adapted strains than in the cold-sensitive strain. The synthesis of CAPs (cold acclimation proteins) was observed under continuous growth at 5³C in all three strains capable of growth at this temperature. Levels of survival after 24 h at 380³C where higher in cold-(79%) and poorly adapted (64%) strains than in the cold-sensitive strain (33%), but a 2-h acclimation period at 5³C before freezing doubled the survival of the cold-sensitive strain. Low temperature conditions affected similarly the fatty acid composition of all strains, regardless of their cold adaptation level. The proportion of unsaturated fatty acids increased significantly with the lowering of growth temperature from 25 to 5³C, but showed a tendency to decrease after a cold shock from 25 to 5³C. A specific unsaturated fatty acid, cis-12 octadecanoic acid, was produced during growth at 5³C. The unsaturated cis-vaccenic acid was the principal component under all conditions. The cold adaptation trait was weakly reflected in symbiosis with the agronomic legume, Lathyrus sativus, with which one coldadapted strain showed a slightly higher nitrogenase activity and shoot dry matter yield than a commercial strain under a sub-optimal temperature regime. ß

Arctic and subarctic soil populations of Rhizobium leguminosarum biovar trifolii nodulating three different clover species: Characterisation by diversity at chromosomal and symbiosis loci

“…viciae has been isolated, and the genetic diversity of the population was as high as in a population from the boreal zone (Drouin et al, 1996). Some of these arctic isolates were cold-adapted and even grew at 0°C (Drouin et al, 2000).…”

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“…By contrast, much less is known about how and why different bacterial strains and species vary in their capacity to survive these stresses [28,35,42,43]. In this study, we examine how evolutionary adaptation by populations of E. coli to serial propagation on a minimal glucose medium at a constant temperature of 37°C affected survival during prolonged freezing and repeated freeze-thaw cycles in the absence of cryoprotectant.…”

Stress Physiology in Azospirillum and Other PGPRs

“…ATCC10317, SEMIA6144, and TAL1371 strains) high growth temperature (37 °C) provoked a slightly reduced biomass production, an increase in the cellular content of low molecular weight oligosaccharides and fully suppressed the synthesis of neutral glucans (Dardanelli et al 1997 ). The FA composition profi les of Bradyrhizobium and Rhizobium are quite different, and effects of growth phase (Boumahdi et al 1999 ; and low temperature (Drouin et al 2000 ;Théberge et al 1996 ) on FA synthesis and composition in these genera have been studied, but not the effects of high temperature or high salinity. In peanut rhizobia ( Bradyrhizobium sp.…”