Physiological amino acid data management: Quantitation, assessment, reporting and storage

Carter, Robert J.; Lukey, Trevor; Snyder, Floyd F.

doi:10.1016/0010-4825(88)90060-1

Cited by 1 publication

(1 citation statement)

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“…[2,3] Plasma amino acids were quantitated using the Biochrom 20 or 30 amino acid analyzers with ninhydrin derivatisation, using physiological amino acid standards (Sigma-Aldrich, Canada) and S-(2-aminoethyl)-L-cysteine as internal standard. [4,5] Plasma methylmalonic acid was analyzed on a HewlettPackard GC-MS, using methyl-d3-malonic acid as an internal standard (CDN Isotopes, Canada). [6] …”

Section: Methodsmentioning

confidence: 99%

Application of Metabolomic Principles to Disorders of Nucleotide Metabolism Reveals New Metabolic Perturbations

Snyder

Carter

Fung

et al. 2008

Nucleosides, Nucleotides and Nucleic Acids

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A metabolomic analysis of plasma amino acids and acylcarnitines was applied to four disorders of nucleotide metabolism. Multivariate analysis gave score plots that show segregation of hypoxanthine phosphoribosyltransferase and adenine phosphoribosyltransferase deficient plasma from controls with equivocal results for adenosine deaminase and dihydropyrimidine dehydrogenase deficiencies. Loadings plots revealed the principal metabolites responsible for the discrimination between these classes. There were increases for HPRT in C4-, C6-, and C3-DC (malonyl)-carnitines, and decreased serine. For APRT there were increases in C4- to C10- and C3-DC to C6-DC-carnitines, urea, 1-methylhistidine, 3-methylhistidine, and decreased tryptophan. For ADA deficiency there were increases in C4- and C6-carnitines, taurine, and isoleucine.

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