Physiological and biochemical features of embryogenic and non-embryogenic peach palm (Bactris gasipaes Kunth) cultures

Nascimento-Gavioli, Maria Carolina Andrade; Cangahuala-Inocente, Gabriela Claudia; Steinmacher, Douglas André; Ree, Joseph Francis; Steiner, Neusa; Guerra, Miguel Pedro

doi:10.1007/s11627-017-9805-x

Cited by 9 publications

(4 citation statements)

References 61 publications

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“…According to Fehér et al (2003), this increase in the concentration of total amino acids in developing tissues may have occurred because of the increased metabolic activity of the cultures, stimulated by the presence of auxins in the nutrient medium, which caused biochemical and physiological changes in the plant cells. A high content of total free amino acids was also recently highlighted by Nascimento-Gavioli et al (2017) in embryogenic calli of Bactris gasipaes, which according to the authors, may indicate increased metabolic activity aimed at protein production.…”

Section: Total Proteinsmentioning

confidence: 76%

Somatic embryogenesis and plant regeneration in Piper aduncum L

Sousa

Meira

et al. 2020

In Vitro Cell.Dev.Biol.-Plant

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An efficient protocol is reported for in vitro plant regeneration through somatic embryogenesis in Piper aduncum, a Brazilian Amazon species with high economic potential. The species is important due to a variety of components found in its essential oil, with emphasis on dillapiole. Leaf explants from five accessions identified for high oil yield and levels of dillapiole were evaluated for their embryogenic potential. To induce embryogenic calli, the explants were cultivated in MS medium supplemented with 5 mg L −1 of 1-naphthaleneacetic acid (NAA) and 2.5 mg L −1 of N6-benzylaminopurine (BAP) for 80 d. For somatic embryogenesis, the embryogenic calli were transferred to MS medium with 10 mg L −1 of NAA and 2.5 mg L 1 of BAP and incubated for 45 d. The obtained somatic embryos were germinated in MS medium without regulators by 45 d and the obtained plantlets were subjected to acclimatization. Somatic embryos and calli from this process were subjected to anatomical and histochemical analyses. Biochemical analyses (total soluble sugars, starch, total amino acids, and proteins) were also performed to identify markers for embryogenic competence acquisition. In addition, the germination of somatic embryos was evaluated in a semi-solid and liquid system (R.I.T.A.® temporary immersion bioreactors). The obtained plants were evaluated for genetic fidelity using ISSR markers. The present study indicate that the accessions did not differ in embryogenic potential, with a mean percentage of calli with somatic embryos of 82.4%. Anatomical analyses confirmed the occurrence of the embryogenic route and the histochemical analyses identified starch grains in somatic embryos at different developmental stages. The biochemical analyses showed high total soluble sugars and total amino acids in embryogenic calli, marks of the acquisition of the embryogenic competence of P. aducum. The R.I.T.A.® temporary immersion bioreactors were highly efficient in the regeneration of somatic plants, with 100% germination. The plants regenerated in the semi-solid and liquid systems showed high genetic homogeneity. The survival rate of the acclimatized plants was 100%.

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Section: Total Proteinsmentioning

confidence: 76%

Somatic embryogenesis and plant regeneration in Piper aduncum L

Sousa

Meira

et al. 2020

In Vitro Cell.Dev.Biol.-Plant

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“…For example, in studies of P. nigra [ 15 ] and P. sylvestris [ 16 ], a high spermidine (Spd) level in the callus was used as a physiological marker of SE maturation and cell proliferation. High levels of indole-3-acetic acid (IAA) and abscisic acid (ABA) were typical characteristics of Bactris gasipaes and Triticum aestivum embryogenic calli [ 17 , 18 ]. High levels of reactive oxygen species (ROS), ethylene, and ROS-related proteins detected in A. angustifolia were associated with cell lines with high somatic embryogenesis potential [ 7 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Morphological and Physiological Indicators for Screening Cell Lines with High Potential for Somatic Embryo Maturation at an Early Stage of Somatic Embryogenesis in Pinus Koraiensis

Peng

Gao

Wang

et al. 2022

Plants

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Many cell lines in the embryogenic callus cannot produce somatic embryos (SEs) even if they meet the optimal SE maturation culture conditions during conifer somatic embryogenesis. This phenomenon hinders the progress of the industrial-scale reproduction of conifers. Therefore, there is an urgent need to obtain morphological and physiological markers to screen embryogenic calli in response to SE maturation conditions. To detect cell lines with high somatic embryogenesis potential during the proliferation process, we counted the number of pro-embryos and early SEs (ESEs) in different cell lines and storage substances, endogenous hormones, and polyamine contents. The results showed that the yield of P. koraiensis SEs was heavily dependent on genotype (p = 0.001). There were high levels of PE III (pro-embryo III) number, ESE number, and soluble protein content, in the response cell lines (R cell lines), which were 1.6-, 3-, and 1.1-fold those of the obstructive cell lines (B cell lines), respectively. The B cell line had high levels of starch, auxin (IAA), Put, Spd, and putrescine: spermine (Put: Spm) compared to the R cell line. In addition, the numbers of PE III, ESEs, and soluble protein content were significantly positively correlated with SE yield. In contrast, the contents of starch, abscisic acid (ABA), Put, Spm, and Spd were significantly negatively correlated with SE yield. To ensure the accuracy of the results, we used nine cell lines to test the results. The PE III and ESE numbers and the Spm and Spd contents were positively correlated with SE yield, while the levels of starch, ABA, IAA, Put: Spd, and Put: Spm were negatively correlated with SE yield. Thus, we recommend using high PE III and ESEs as morphological indicators and low levels of starch, IAA, ABA, and Put: Spm as physiological markers to screen cell lines with a high somatic embryogenesis potential. In addition, we also found that the relationship between Spd, Spm, and SE yield was opposite in the two experimental results. Therefore, we speculate that the differences in Spd and Spm content are mainly affected by genotype. In conclusion, this study obtained the morphological and physiological markers of some high-somatic embryogenic cell lines by comparing the differences between nine somatic embryogenic cell lines. Our results can guide the improvement of conifer somatic embryogenesis technology and can provide a theoretical basis for accelerating the application of biotechnology in large-scale artificial breeding.

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“…Similarly, embryogenic lines of P. nigra presented spermine as the lowest abundant PA (Noceda et al, 2009). Putrescine was also the most abundant PA found in embryogenic cultures of Bactris gasipaes (Nascimento-Gavioli et al, 2017) sugarcane callus (Silveira et al, 2013). According to Bagni and Tassoni (2001), putrescine and spermidine are generally the most abundant PAs in plants, while spermine is only present in small levels.…”

Section: Changes In Free Endogenous Polyaminesmentioning

confidence: 92%

<b>Dynamics in global DNA methylation and endogenous polyamine levels during protocorm-like bodies induction of <i>Cattleya tigrina</i> A. Richard

Almeida

Fraga

Navarro

et al. 2017

Acta Sci. Biol. Sci.

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ABSTRACT. Cattleya tigrina is endemic to the Atlantic forest biome and classified as vulnerable in the Red Book of Brazilian Flora. In vitro techniques comprise valuable tools for the conservation of endangered plant species. The aim of the present study was to evaluate the morphological features, global DNA methylation levels and free polyamines during protocorm-like bodies (PLBs) induction of C. tigrina. Along with that, an efficient protocol for in vitro propagation of this species is proposed. The first evidences of PLBs induction in C. tigrina occurred at seven days in culture, starting from the basal portion of the leaf abaxial surface. A hypomethylation marked the beginning of cell differentiation, followed by an increased global DNA methylation at 35 days in culture, coinciding with a subtle change in the structures morphogenetic development. During PLBs induction, putrescine exhibited higher levels as compared to spermidine and spermine, and apparently presents a major role during the PLBs induction in C. tigrina. Due to the apparent secondary PLBs formation, this protocol can represent a highly efficient method for in vitro propagation of this species.Keywords: orchids, micropropagation, in vitro culture, epigenetics, PLBs.Dinâmica da metilação do DNA global e níveis endógenos de poliaminas durante a indução de estruturas semelhantes a protocormos de Cattleya tigrina A. Richard RESUMO. Cattleya tigrina é uma espécie endêmica do bioma Mata Atlântica e classificada como vulnerável no Livro Vermelho da Flora Brasileira. As técnicas in vitro compreendem ferramentas valiosas a serem empregadas na conservação de espécies de plantas ameaçadas. O objetivo do presente estudo foi avaliar as características morfológicas, os níveis globais de metilação do DNA e as poliaminas livres durante a indução de estruturas semelhantes a protocormos (ESPs). Paralelamente, um protocolo eficiente para a propagação in vitro desta espécie é apresentado. As primeiras evidências de indução de ESPs em C. tigrina foram observadas aos sete dias de cultivo, a partir da porção basal da superfície abaxial da folha. Uma hipometilação foi observada concomitante ao início da diferenciação celular, e um aumento da metilação global do DNA foi encontrada aos 35 dias de cultivo, coincidindo com uma sutil mudança no desenvolvimento morfogenético das estruturas. Durante a indução de ESPs, a putrescina exibiu níveis aumentados em comparação a espermidina e espermina e, aparentemente, apresenta um papel importante durante a indução dessas estruturas em C. tigrina. Devido à aparente formação secundária de ESPs, este protocolo pode representar um método altamente eficiente para a propagação in vitro desta espécie.Palavras-chave: orquideas, micropropagação, cultivo in vitro, epigenética, ESPs.

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Physiological and biochemical features of embryogenic and non-embryogenic peach palm (Bactris gasipaes Kunth) cultures

Cited by 9 publications

References 61 publications

Somatic embryogenesis and plant regeneration in Piper aduncum L

Somatic embryogenesis and plant regeneration in Piper aduncum L

Morphological and Physiological Indicators for Screening Cell Lines with High Potential for Somatic Embryo Maturation at an Early Stage of Somatic Embryogenesis in Pinus Koraiensis

<b>Dynamics in global DNA methylation and endogenous polyamine levels during protocorm-like bodies induction of <i>Cattleya tigrina</i> A. Richard

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