Physiological and biochemical responses of the freshwater green algae Closterium ehrenbergii to the common disinfectant chlorine

Sathasivam, Ramaraj; Ebenezer, Vinitha; Guo, Ruoyu; Ki, Jang‐Seu

doi:10.1016/j.ecoenv.2016.08.004

Cited by 38 publications

(18 citation statements)

References 47 publications

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“…In addition, it has been used in toxicity evaluations and bioassays for detecting the deleterious effects of hazardous substances on aquatic systems, due to its sensitivity to a wide range of surfactants . More recently, we found a considerable increase of antioxidant enzyme activities in C. ehrenbergii exposed to environmental stressors . These findings have supported that it might be used as an ideal organism for eco‐toxicological assessment, in terms of physiological and biochemical perspective.…”

Section: Introductionsupporting

confidence: 72%

Small heat shock protein genes of the green algae Closterium ehrenbergii: Cloning and differential expression under heat and heavy metal stresses

Abassi

Wang

Ponmani

et al. 2019

Environmental Toxicology

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The freshwater green algae Closterium ehrenbergii has been considered as a model for eco‐toxicological assessment in aquatic systems. Heat shock proteins (HSPs) are a class of highly conserved proteins produced in all living organisms, which participate in environmental stress responses. In the present study, we determined the cDNA sequences of small heat shock protein 10 (sHSP10) and sHSP17.1 from C. ehrenbergii, and examined the physiological changes and transcriptional responses of the genes after exposure to thermal shock and toxicants treatments. The open reading frame (ORF) of CeHSP10 was 300 bp long, encoding 99 amino acid (aa) residues (10.53 kDa) with a GroES chaperonin conserved site of 22 aa. The CeHSP17.1 had a 468 bp ORF, encoding 155 aa with a conserved C‐terminal α‐crystallin domain. For heat stress, cells presented pigment loss and possible chloroplast damage, with an up‐regulation in the expression of both sHSP10 and sHSP17.1 genes. As for the heavy metal stressors, an increase in the production of reactive oxygen species was registered in a dose dependent manner, with a significant up‐regulation of both sHSP10 and sHSP17.1 genes. These results suggest that sHSP genes in C. ehrenbergii may play a role in responses to stress environments, and they could be used as an early detection parameter as biomarker genes in molecular toxicity assessments.

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Section: Introductionsupporting

confidence: 72%

Small heat shock protein genes of the green algae Closterium ehrenbergii: Cloning and differential expression under heat and heavy metal stresses

Abassi

Wang

Ponmani

et al. 2019

Environmental Toxicology

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show abstract

“…The growth inhibition increased with the increasing of exposure time. This result was consistent with other previous reports, in which the toxicity of lead [16], cadmium [17] and chlorine [18] to algae was related to the incubation time. After 96 h exposure, the inhibition rates relative to the control group of 7.2, 10.8, and 14.4 μM Th were 22.5%, 55.1%, and 67.8%, respectively.…”

Section: Resultssupporting

confidence: 94%

Influence of Speciation of Thorium on Toxic Effects to Green Algae Chlorella pyrenoidosa

Peng

Ding

et al. 2017

IJMS

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Thorium (Th) is a natural radioactive element present in the environment and has the potential to be used as a nuclear fuel. Relatively little is known about the influence and toxicity of Th in the environment. In the present study, the toxicity of Th to the green algae Chlorella pyrenoidosa (C. pyrenoidosa) was evaluated by algal growth inhibition, biochemical assays and morphologic observations. In the cultural medium (OECD TG 201), Th(NO3)4 was transformed to amorphous precipitation of Th(OH)4 due to hydrolysis. Th was toxic to C. pyrenoidosa, with a 96 h half maximum effective concentration (EC50) of 10.4 μM. Scanning electron microscopy shows that Th-containing aggregates were attached onto the surface of the algal cells, and transmission electron microscopy indicates the internalization of nano-sized Th precipitates and ultrastructural alterations of the algal cells. The heteroagglomeration between Th(OH)4 precipitation and alga cells and enhanced oxidative stress might play important roles in the toxicity of Th. To our knowledge, this is the first report of the toxicity of Th to algae with its chemical species in the exposure medium. This finding provides useful information on understanding the fate and toxicity of Th in the aquatic environment.

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“…The growth inhibition increased with the increasing of exposure time. This result was consistent with other previous reports, in which the toxicity of lead [21], cadmium [22] and chlorine [23] to algae was related to the incubation time. After 96 h exposure, the inhibition rates relative to the control group of 7.2, 10.8, and 14.4 μM Th were 22.5, 55.1, and 67.8%, respectively.…”

Section: Chemical Species Of Th In the Mediasupporting

confidence: 83%

Influence of Speciation of Thorium on Toxic Effects to Green Algae <em>Chlorella pyrenoidosa</em>

Peng

Ding

et al. 2017

Preprint

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Thorium (Th) is a natural radioactive element present in the environment and has a potential to be used as a nuclear fuel. Relatively little is known about the influence and toxicity of Th in the environment. In the present study, the toxicity of Th to the green algae Chlorella pyrenoidosa (C. Pyrenoidosa) was evaluated by algal growth inhibition, biochemical assays and morphologic observations. In the cultural medium (OECD TG 201), Th(NO3)4 was transformed to amorphous precipitation of Th(OH)4 due to hydrolysis. Th was toxic to C. Pyrenoidosa, with a 96 h half maximum effective concentration (EC50) of 10.4 μM. Scanning electron microscopy shows that Th-containing aggregates were attached onto the surface of the algal cells, and transmission electron microscopy indicates the internalization of nano-sized Th precipitates and ultrastructural alterations of the algal cells. The heteroagglomeration between Th(OH)4 precipitation and alga cells and enhanced oxidative stress might play important roles in the toxicity of Th. To our knowledge, this is the first report of the toxicity of Th to algae with its chemical species in the exposure medium. This finding provides useful information on understanding the fate and toxicity of Th in the aquatic environment.

show abstract

Physiological and biochemical responses of the freshwater green algae Closterium ehrenbergii to the common disinfectant chlorine

Cited by 38 publications

References 47 publications

Small heat shock protein genes of the green algae Closterium ehrenbergii: Cloning and differential expression under heat and heavy metal stresses

Small heat shock protein genes of the green algae Closterium ehrenbergii: Cloning and differential expression under heat and heavy metal stresses

Influence of Speciation of Thorium on Toxic Effects to Green Algae Chlorella pyrenoidosa

Influence of Speciation of Thorium on Toxic Effects to Green Algae <em>Chlorella pyrenoidosa</em>

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