Physiological and Cellular Stress Responses of Juvenile Rainbow Trout to Vibriosis

Ackerman, Paige A.; Iwama, George K.

doi:10.1577/1548-8667(2001)013<0173:pacsro>2.0.co;2

Cited by 99 publications

(54 citation statements)

References 32 publications

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“…Vibriosis has been associated with anemia; for example, Harbell et al (1979) noted Hct dropped from 37 to 15% as coho salmon Oncorhynchus kisutch became moribund 48 h following Listonella anguillarum exposure. Conversely, and in agreement with our findings, Ackerman & Iwama (2001), who used an injection protocol similar to the one we used, found no change in Hct up to 7 d post-injection. Lct was more variable in saline-injected control fish, with normal Lct in 3 of the 5 (Wedemeyer et al 1983), but a negligible Lct in 2 other fish.…”

supporting

confidence: 92%

“…Five fish were given the low dose (1.67 × 10 5 ± 1.53 × 10 4 CFUs), and 6 fish were given the 100-fold higher dose (2.36 × 10 7 ± 4.00 × 10 6 CFUs). The low dose was chosen because it had proven efficacious at inducing vibriosis in rainbow trout in earlier work (Ackerman et al 1999, Ackerman & Iwama 2001. To control for any effects of the injection or treatment system, 5 additional fish were injected with 0.1 ml sterile saline alone (0.85% NaCl).…”

mentioning

confidence: 99%

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Subclinical Listonella anguillarum infection does not impair recovery of swimming performance in rainbow trout Oncorhynchus mykiss

Tierney

Balfry²,

Farrell³

2005

Dis. Aquat. Org.

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This study examines whether injections of the commonly used bacterial-challenge pathogen Listonella anguillarum (formerly Vibrio anguillarum) negatively impact the ability of rainbow trout Oncorhynchus mykiss Walbaum to perform repeat swimming trials. Fish were given intraperitoneal injections of either a sub-lethal (10 5 colony forming units; CFUs) or a lethal (10 7 CFUs) dose of L. anguillarum, held for 48 h, and then given 2 successive ramp critical swimming speed (U crit ) tests separated by 45 min. Compared with saline-injected control fish, the low-dose injection did not significantly impair swimming performance and recovery. Similarly, U crit and re-performance for fish surviving the high-dose injection were comparable to control (2 of 6 fish died after injection and before testing). In contrast, a positive control test of seawater challenge did impair recovery of swimming performance. In view of these results and common use of L. anguillarum as a challenge pathogen for toxicological studies, it seems unlikely that the consequences of pathogenesis impact the important cardiorespiratory changes associated with exercise.KEY WORDS: Listonella anguillarum · Vibriosis · Seawater · Swimming performance · Recovery · Critical swimming speed · U crit Resale or republication not permitted without written consent of the publisherDis Aquat Org 67: [81][82][83][84][85][86] 2005 been tested. Therefore, the principle aim of this study was to examine the effect of L. anguillarum injections on rainbow trout swimming and recovery ability using repeated ramp-U crit testing (Jain et al. 1997). MATERIALS AND METHODSFish. Rainbow trout (mass 379 ± 12 g, fork length 29.8 ± 0.3 cm) were acquired from Sun Valley Trout Farm (Mission, BC) and maintained in flow-through dechlorinated municipal water (temperature 8.9 ± 0.3°C; dissolved oxygen > 8 mg O 2 l -1 ) at the Department of Fisheries and Oceans/University of British Columbia (DO/UBC) Centre for Aquaculture and Environmental Research (West Vancouver, BC). Fish were fed commercial trout chow ad libitum once daily.Swimming performance protocol. Swimming measurements were performed in a 215 l, mobile Bretttype respirometer described in Farrell et al. (2003). Individual trout were taken to stage 3 anesthesia (Summerfelt & Smith 1990) ), which allowed fish to remain stationary on the bottom of the chamber without swimming. After the recovery period, trout were given a conditioning swim that consisted of an abbreviated step velocity test (described in Jain et al. 1997). For the conditioning swim, water speed was increased in regular increments of 0.15 BL s -1 every 2 min until the fish was unwilling to swim faster and rested on the electric grid at the rear of the swim chamber. The conditioning swim was intended to eliminate the potential training effect of repeat swimming (Farlinger & Beamish 1978, Jain et al. 1997 and also provide an index of individual performance to set the ramp velocities. Following acclimation and beginning at approximately the same time of day (...

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supporting

confidence: 92%

mentioning

confidence: 99%

Subclinical Listonella anguillarum infection does not impair recovery of swimming performance in rainbow trout Oncorhynchus mykiss

Tierney

Balfry²,

Farrell³

2005

Dis. Aquat. Org.

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“…An increased expression of Hsp70 gene in liver and kidney tissues of Coho salmon, Oncorhynchus kisutch during Renibacterium salmoninarum infection up to 63 days post-exposure (Forsyth et al 1997) was the first report to explain the influence of heat shock protein genes in fish in response to pathogens. Hsp70 gene was also over-expressed in rainbow trout after acute Vibrio anguillarum challenge (Ackerman and Iwama 2001). Induced expression of Hsp70 gene was noticed in sea bream liver tissue at 36 h post-infection with Vibrio alginolyticus (Deane et al 2004).…”

Section: Discussionmentioning

confidence: 93%

Expression analysis of heat shock protein genes during Aeromonas hydrophila infection in rohu, Labeo rohita, with special reference to molecular characterization of Grp78

Das

Mohapatra

Sahoo

2015

Cell Stress and Chaperones

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“…In teleost, hsp70 genes have been found to be involved in bacterial kidney disease in coho salmon [28] and vibriosis in rainbow trout [29]. In olive flounder, Hsp40 proteins were found to be upregulated in flounder embryonic cells (FEC) after viral infection and a flounder hsp70 gene was also expressed in heat-shocked and virus treated FEC cells [30], indicating hsp40 and hsp70 functioned as co-chaperone in antiviral immune responses.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification of Hsp70 genes in channel catfish and their regulated expression after bacterial infection

Song

Xie

et al. 2016

Fish & Shellfish Immunology

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Physiological and Cellular Stress Responses of Juvenile Rainbow Trout to Vibriosis

Cited by 99 publications

References 32 publications

Subclinical Listonella anguillarum infection does not impair recovery of swimming performance in rainbow trout Oncorhynchus mykiss

Subclinical Listonella anguillarum infection does not impair recovery of swimming performance in rainbow trout Oncorhynchus mykiss

Expression analysis of heat shock protein genes during Aeromonas hydrophila infection in rohu, Labeo rohita, with special reference to molecular characterization of Grp78

Genome-wide identification of Hsp70 genes in channel catfish and their regulated expression after bacterial infection

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