Physiological and comparative genomic analysis of new isolated yeasts Spathaspora sp. JA1 and Meyerozyma caribbica JA9 reveal insights into xylitol production

Trichez, Débora; Steindorff, Andrei Stecca; Soares, Carlos E V F; Formighieri, Eduardo Fernandes; Almeida, J. R. M. de

doi:10.1093/femsyr/foz034

Cited by 25 publications

(20 citation statements)

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“…The analyses of xylose reductase activity with NADH and NADPH in the selected C. tropicalis strain JA2 was carried out by Trichez, Steindorff, Soares, Formighieri, and Almeida (), and the results showed that the xylose reductase (XR) activity was strictly NADPH‐dependent. High XR activities improve the rate of xylose consumption and when associated with dependence on the NADPH cofactor, they favor the production of xylitol (Cadete et al, ; Karhumaa, Garcia‐Sanchez, Hahn‐Hägerdal, & Gorwa‐Grauslund, ).…”

Section: Resultsmentioning

confidence: 99%

Xylitol production on sugarcane biomass hydrolysate by newly identified Candida tropicalis JA2 strain

Morais

Pacheco

Trichez

et al. 2019

Yeast

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Xylitol is a building block for a variety of chemical commodities, besides being widely used as a sugar substitute in the food and pharmaceutical industries. The aim of this work was to develop a microbial process for xylitol production using sugarcane bagasse hydrolysate as substrate. In this context, 218 non-Saccharomyces yeast strains were screened by growth on steam-exploded sugarcane bagasse hydrolysate containing a high concentration of acetic acid (8.0 g/L). Seven new Candida tropicalis strains were selected and identified, and their ability to produce xylitol on hydrolysate at low pH (4.6) under aerobic conditions was evaluated.The most efficient strain, designated C. tropicalis JA2, was capable of producing xylitol with a yield of 0.47 g/g of consumed xylose. To improve xylitol production by C. tropicalis JA2, a series of experimental procedures were employed to optimize pH and temperature conditions, as well as nutrient source, and initial xylose and inoculum concentrations. C. tropicalis JA2 was able to produce 109.5 g/L of xylitol with a yield of 0.86 g/g of consumed xylose, and with a productivity of 2.81 g·L·h, on sugarcane bagasse hydrolysate containing 8.0 g/L acetic acid and177 g/L xylose, supplemented with 2.0 g/L yeast nitrogen base and 4.0 g/L urea. Thus, it was possible to identify a new C. tropicalis strain and to optimize the xylitol production process using sugarcane bagasse hydrolysate as a substrate. The xylitol yield on biomass hydrolysate containing a high concentration of acetic acidobtained in here is among the best reported in the literature.

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Section: Resultsmentioning

confidence: 99%

Xylitol production on sugarcane biomass hydrolysate by newly identified Candida tropicalis JA2 strain

Morais

Pacheco

Trichez

et al. 2019

Yeast

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“…Yeasts can produce considerable amounts of xylitol, however each species and even different strains of the same species present peculiarities regarding production efficiencies [7,8]. Candida species have been recognized as the best xylitol producers, reaching yield and productivity of Y P/S = 0.84 g/g and P = 1.01 g/L h − ¹, respectively [1].…”

Section: Introductionmentioning

confidence: 99%

“…However, Candida species are considered opportunistic pathogens, and their utilization in biotechnological processes have been questioned due to the absence of a GRAS status (generally recognized as safe) [1,2,3]. In this context, several yeast species have been screened and identified, including species of Meyerozyma [9], Spathaspora [8], Scheffersomyces [10], Debaromyces [11], and Kluyveromyces [12]. Recently, the Spathaspora sp.…”

Section: Introductionmentioning

confidence: 99%

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Xylitol Production: Identification and Comparison of New Producing Yeasts

2019

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Xylitol is a sugar alcohol with five carbons that can be used in the pharmaceutical and food industries. It is industrially produced by chemical route; however, a more economical and environmentally friendly production process is of interest. In this context, this study aimed to select wild yeasts able to produce xylitol and compare their performance in sugarcane bagasse hydrolysate. For this, 960 yeast strains, isolated from soil, wood, and insects have been prospected and selected for the ability to grow on defined medium containing xylose as the sole carbon source. A total of 42 yeasts was selected and their profile of sugar consumption and metabolite production were analyzed in microscale fermentation. The six best xylose-consuming strains were molecularly identified as Meyerozyma spp. The fermentative kinetics comparisons on defined medium and on sugarcane bagasse hydrolysate showed physiological differences among these strains. Production yields vary from YP/S = 0.25 g/g to YP/S = 0.34 g/g in defined medium and from YP/S = 0.41 g/g to YP/S = 0.60 g/g in the hydrolysate. Then, the xylitol production performance of the best xylose-consuming strain obtained in the screening, which was named M. guilliermondii B12, was compared with the previously reported xylitol producing yeasts M. guilliermondii A3, Spathaspora sp. JA1, and Wickerhamomyces anomalus 740 in sugarcane bagasse hydrolysate under oxygen-limited conditions. All the yeasts were able to metabolize xylose, but W. anomalus 740 showed the highest xylitol production yield, reaching a maximum of 0.83 g xylitol/g of xylose in hydrolysate. The screening strategy allowed identification of a new M. guilliermondii strain that efficiently grows in xylose even in hydrolysate with a high content of acetic acid (~6 g/L). In addition, this study reports, for the first time, a high-efficient xylitol producing strain of W. anomalus, which achieved, to the best of our knowledge, one of the highest xylitol production yields in hydrolysate reported in the literature.

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“…Candida intermedia, Candida tropicalis and Pichia kudriavzevii have been reported to produce ethanol and single-cell protein [52][53][54][55] whereas Wickerhamomyces anomalus has been found to have the ability to produce ethanol and xylitol [54,62]. Meyerozyma caribbica has exhibited potential for xylitol production [64]. Various species in the Basidiomycota found in this study including P. laurentii, R. paludigena, Rhodotorula toruloides, Rhodosporidiobolus fluvialis, Rhodosporidiobolus ruineniae and Sporobolomyces carnicolor have been reported for their potential in microbial lipid production [56][57][58].…”

Section: Discussionmentioning

confidence: 99%

Yeast Diversity Associated with the Phylloplane of Corn Plants Cultivated in Thailand

et al. 2020

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The ecology and diversity of phylloplane yeasts is less well understood in tropical regions than in temperate ones. Therefore, we investigated the yeast diversity associated with the phylloplane of corn, an economically important crop in Thailand, by a culture-dependent method. Thirty-six leaf samples were collected and 217 yeast strains were isolated by plating leaf-washings. The strains were grouped by PCR-fingerprinting and representative strains were identified by analysis of the D1/D2 domain of the large subunit rRNA gene. In total, 212 strains were identified within 10 species in the Ascomycota and 32 species in the Basidiomycota. Five strains represented potential new species in the Basidiomycota, one strain was recently described as Papiliotrema plantarum, and four strains belonged to the genera Vishniacozyma and Rhodotorula. A higher number of strains in the Basidiomycota (81.6%) was obtained. Hannaella sinensis was the species with the highest occurrence. Principal coordinates analysis ordinations of yeast communities revealed that there were no differences in the similarity of the sampling sites. The estimation of the expected species richness showed that the observed species richness was lower than expected. This work indicated that a majority of yeast associated with the phylloplane of corn plant belongs to the phylum Basidiomycota.

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Physiological and comparative genomic analysis of new isolated yeasts Spathaspora sp. JA1 and Meyerozyma caribbica JA9 reveal insights into xylitol production

Cited by 25 publications

References 76 publications

Xylitol production on sugarcane biomass hydrolysate by newly identified Candida tropicalis JA2 strain

Xylitol production on sugarcane biomass hydrolysate by newly identified Candida tropicalis JA2 strain

Xylitol Production: Identification and Comparison of New Producing Yeasts

Yeast Diversity Associated with the Phylloplane of Corn Plants Cultivated in Thailand

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