Physiological and DNA methylation analysis provides epigenetic insights into chromium tolerance in kenaf

Tang, Meiqiong; Jiao, Yue; Huang, Zhen; Hu, Yijie; Li, Zengqiang; Luo, Dengjie; Cao, Shan; Zhang, Hui; Pan, Jiao; Wu, Xia; Wu, Qijing; Chen, Peng

doi:10.1016/j.envexpbot.2021.104684

Cited by 24 publications

(16 citation statements)

References 64 publications

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“…In addition, they reported that proteome and the transcripts associated with the guard cells showed a positive correlation between the highest accumulation of proteins and genes with the highest percentages of 5-mC in the vegetative stage and the vegetative and at the reproductive stages. Furthermore, an increase in the DNA methylation associated with phenotypic changes, such as higher antioxidant activity in Hibiscus cannabinus L. seedlings was related to chromium tolerance mechanisms, and these changes then affected the expression of speci c genes involved in the chromium stress response (Tang et al 2021). In this sense, several works reported that DNA methylation modulates the expression of various genes and consequently phenotypic changes in response to stressful environmental conditions (He et ).…”

Section: Discussionmentioning

confidence: 99%

High temperature and water deficit cause epigenetic changes in somatic plants of Pinus radiata D. Don

Nascimento

Montalbán

Miguel

et al. 2022

Plant Cell Tiss Organ Cult

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Current climate changes imply an imminent risk for forest species. In this context, somatic embryogenesis is a valuable tool to study the response of plants to different abiotic stresses. Based on this, we applied a high-temperature regime (50 ºC, 5 min) during the maturation of Pinus radiata D. Don embryogenic masses in order to evaluate the development of an epigenetic memory months later.Therefore, somatic plants (SP) resulting from somatic embryos (ses) maturated at control temperature and cultivated in a greenhouse were submitted to heat stress (40 ºC, 2 h, 10 days; 23 ºC, 10 days) or at a control temperature (23 ºC, 20 days); while another 20 SP resulting from ses maturated in the two temperature regimes and cultivated in the greenhouse were submitted to drought stress or weekly irrigated. All plants were evaluated for relative water content, water potential, electrolyte leakage, stomatal conductance, transpiration, methylation (5-mC) and hydroxymethylation (5-hmC) levels. The results showed that the SP obtained from ses maturated at 50 ºC showed an adaptation to drought stress based on water potential and transpiration. Furthermore, SP kept under heat stress in a greenhouse showed lower 5-hmC levels than SP kept at 23 ºC. Furthermore, the 5-hmC and 5-hmC/5-mC ratio showed a signi cantly negative correlation with changes in water potential; and a signi cantly negative correlation was observed between the levels of stomatal conductance and 5-mC. We conclude that the manipulation of conditions during the maturation process in somatic embryogenesis modulates the physiological characteristics of the SP obtained. Key MessageApplication of high temperatures in the maturation stage of somatic embryos and the resulting Pinus radiata somatic plants provoked a drought adaptation and changes in epigenetic mechanisms.

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Section: Discussionmentioning

confidence: 99%

High temperature and water deficit cause epigenetic changes in somatic plants of Pinus radiata D. Don

Nascimento

Montalbán

Miguel

et al. 2022

Plant Cell Tiss Organ Cult

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“…The total genomic DNA of samples mentioned above were isolated using modified CTAB method ( Doyle and Doyle, 1987 ). The MSAP procedure is performed to investigate global DNA methylation changes according to an established protocol ( Tang et al, 2022 ). Two restriction enzyme combinations, Eco RI/ Hpa II and Eco RI/ Msp I (Thermo Fisher Scientific, United States), were used for digestion.…”

Section: Methodsmentioning

confidence: 99%

“…The MSAP profiles showing reproducible results between replicates, ranging from 100 to 500 bp, were scored and transformed into a 1/0 binary matrices, where 1 indicates the presence and 0 the absence of a given fragment. Four types of MSAP bands were defined as non-methylation, hemi-methylation, and full-methylation ( Table 2 ) according to Tang et al (2022) .…”

Section: Methodsmentioning

confidence: 99%

The Mutational, Epigenetic, and Transcriptional Effects Between Mixed High-Energy Particle Field (CR) and 7Li-Ion Beams (LR) Radiation in Wheat M1 Seedlings

Zhao

Zhang

et al. 2022

Front. Plant Sci.

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Ionizing radiation (IR) is an effective approach for mutation breeding. Understanding the mutagenesis and transcriptional profiles induced by different mutagens is of great significance for improving mutation breeding efficiency. Here, using RNA sequencing and methylation-sensitive amplification polymorphism (MSAP) approaches, we compared the genetic variations, epigenetics, and transcriptional responses induced by the mixed high-energy particle field (CR) and 7Li-ion beam (LR) radiation in M1 seedlings of two wheat genotypes (Yangmai 18 and Yangmai 20). The results showed that, in both wheat genotypes, CR displayed significantly a higher mutation efficiency (1.79 × 10–6/bp) than that by LR (1.56 × 10–6/bp). The induced mutations were not evenly distributed across chromosomes and varied across wheat genotypes. In Y18 M1, the highest number of mutations were detected on Chr. 6B and Chr. 6D, whilst in Y20 M1, Chr. 7A and Chr. 3A had the highest mutations. The transcript results showed that total of 4,755 CR-regulated and 1,054 LR-regulated differentially expressed genes (DEGs) were identified in the both genotypes. Gene function enrichment analysis of DEGs showed that these DEGs overlapped or diverged in the cascades of molecular networks involved in “phenylpropanoid biosynthesis” and “starch and sucrose metabolism” pathways. Moreover, IR type specific responses were observed between CR an LR irradiation, including specific TFs and response pathways. MSAP analysis showed that DNA methylation level increased in LR treatment, while decreased at CR. The proportion of hypermethylation was higher than that of hypomethylation at LR, whereas a reverse pattern was observed at CR, indicating that DNA methylation plays critical roles in response to IR irradiation. All these results support that the response to different IRs in wheat includes both common and unique pathways, which can be served as a useful resource to better understand the mechanisms of responses to different IRs in other plants.

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“…analysis was performed as previously described (Tang et al, 2022;Xu et al, 2012) Only epiloci consistent among the three biological replicates were used for further analysis.…”

Section: Methylation-sensitive Amplified Polymorphism Analysismentioning

confidence: 99%

“…to indicate the presence or absence of bands, respectively. Four types of MSAP bands were defined as nonmethylation, hemi-methylation and fullmethylation (Table 1) according to Tang et al (2022).…”

Section: Methylation-sensitive Amplified Polymorphism Analysismentioning

confidence: 99%

Comparative transcriptomic and epigenetic analyses reveal conserved and divergent regulatory pathways in barley response to temperature stresses

Yang

et al. 2022

Physiologia Plantarum

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DNA methylation and histone modification enable plants to rapidly adapt to adverse temperature stresses, including low temperature (LT) and high temperature (HT) stress. In this study, we conducted physiological, epigenetic, and transcriptomic analyses of barley seedlings grown under control (22°C), mild low temperature (MLT, 14°C) and HT (38°C) conditions to elucidate the underlying molecular mechanisms. Compared to MLT, HT implies greater deleterious effects on barley seedlings' growth. The methylation‐sensitive amplification polymorphism analysis showed that MLT induced more DNA methylation and HT more DNA demethylation compared to control. Besides, the higher levels of H3K9ac and H3K4me3 under HT compared to MLT stresses might lead to the loosening of chromatin and, subsequently, the activation of gene expression. Consistently, the transcriptome analysis revealed that there were more differentially expressed genes (DEGs) in plants subjected to HT stress than MLT stress compared to control. The common and unique pathways of these DEGs between MLT and HT were also analyzed. Transcription factors, such as ERF, bHLH, NAC, HSF, and MYB, were most involved in MLT and HT stress. The underlying gene regulation networks of epigenetic modulation‐related genes were further explored by weight gene co‐expression network analysis. Our study provides new insights into the understanding of epigenetic regulation responses to temperature stress in barley, which will lead to improved strategies for the development of cold‐ and heat‐tolerant barley varieties for sustainable barley production in a climate‐changing world.

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Physiological and DNA methylation analysis provides epigenetic insights into chromium tolerance in kenaf

Cited by 24 publications

References 64 publications

High temperature and water deficit cause epigenetic changes in somatic plants of Pinus radiata D. Don

High temperature and water deficit cause epigenetic changes in somatic plants of Pinus radiata D. Don

The Mutational, Epigenetic, and Transcriptional Effects Between Mixed High-Energy Particle Field (CR) and 7Li-Ion Beams (LR) Radiation in Wheat M1 Seedlings

Comparative transcriptomic and epigenetic analyses reveal conserved and divergent regulatory pathways in barley response to temperature stresses

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