Physiological and Genetic Variability of Commercial Isolates of Culinary-Medicinal Mushroom Agaricus brasiliensis S. Wasser et al. (Agaricomycetideae) Cultivated in Brazil

Anaerobically digested food waste in compost for Agaricus bisporus and Agaricus subrufescens and its effect on mushroom productivity

“…and 7.5 by González Matute et al . According to Neves et al ., who investigated growth of mycelium on sterilised agar media, no strains of A. subrufescens would grow on substrates with a pH above 8. They recommended pH values around 7 since lower values would encourage moulds.…”

“…In experiment 3 the pH values corresponding to higher yield were around 8, while the top range (8.2-8.25) gave lower yields. Values above 8 are high compared to a substrate pH of 5.07 used by de Andrade et al 36 and 7.5 by González Matute et al 35 According to Neves et al, 37 who investigated growth of mycelium on sterilised agar media, no strains of A. subrufescens would grow on substrates with a pH above 8. They recommended pH values around 7 since lower values would encourage moulds.…”

Fungal Cultivation and Production of Polysaccharides

“…One of the main challenges in maintaining cultures of A. subrufescens is its low culture viability and the frequent changes in growth habits, mainly after subcultures (Neves et al 2005). In addition, some techniques may not present desirable results, as is the case for lyophilization that proved to be inefficient for A. subrufescens, even when 10 % trehalose and 10 % skimmed milk are used.…”

“…It is crucial to observe the genetic stability of the preserved mycelium. The polymerase chain reaction (PCR) and other protocols for DNA analysis, such as random amplified polymorphic DNA (RAPD) markers, have become important tools in evaluating the intraspecific variability in microorganisms (Atienzar et al 2002;Neves et al 2005) and in detecting DNA damages and mutations .…”

“…The RAPD technique has been largely used to detect the genetic variability of pathogenic and nonpathogenic microorganisms (Steindel et al 1993;Oborník et al 2000;Neves et al 2005;Gasser 2006). This method is cheap, simple to perform, and suitable to detect DNA mutations, which can be important for evaluating the genetic stability in microorganisms during in vitro storage (Mariano et al 2007).…”