Physiological and genomic characterisation of Luteimonas fraxinea sp. nov., a bacterial species associated with trees tolerant to ash dieback

Ulrich, Kristina; Becker, Regina; Behrendt, Undine; Kube, Michael; Schneck, Volker; Ulrich, Andreas

doi:10.1016/j.syapm.2022.126333

Cited by 13 publications

(7 citation statements)

References 105 publications

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“…This phenomenon may be related to the high diversity of rhizosphere microorganisms, which leads to potentially high plant growth‐promoting ability (Ling et al, 2022 ). The foliar spray of PGPB stimulated plants to recruit potential functional taxa similar to those in Bacillus soil inoculation such as Rhizobium, Luteimonas and Sphingopyxis , and these taxonomy have been widely reported to promote plant growth through increasing the acquisition of nitrogen and/or secreting IAA (Dias et al, 2009 ; Ulrich et al, 2022 ; Yang et al, 2022 ). Thus, we speculated that PGPB inoculation, both in the soil and phyllosphere, can directly colonize and indirectly stimulate beneficial microorganisms in the rhizosphere to promote plant growth.…”

Section: Discussionmentioning

confidence: 99%

Bacillus velezensisSQR9 promotes plant growth through colonization and rhizosphere–phyllosphere bacteria interaction

Yu,

Wang,

Zhang

et al. 2024

Environ Microbiol Rep

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The rhizosphere and phyllosphere of plants are home to a diverse range of microorganisms that play pivotal roles in ecosystem services. Consequently, plant growth‐promoting bacteria (PGPB) are extensively utilized as inoculants to enhance plant growth and boost productivity. Despite this, the interactions between the rhizosphere and phyllosphere, which are influenced by PGPB inoculation, have not been thoroughly studied to date. In this study, we inoculated Bacillus velezensis SQR9, a PGPB, into the bulk soil, rhizosphere or phyllosphere, and subsequently examined the bacterial communities in the rhizosphere and phyllosphere using amplicon sequencing. Our results revealed that PGPB inoculation increased its abundance in the corresponding compartment, and all treatments demonstrated plant growth promotion effects. Further analysis of the sequencing data indicated that the presence of PGPB exerted a more significant impact on bacterial communities in both the rhizosphere and phyllosphere than in the inoculation compartment. Notably, the PGPB stimulated similar rhizosphere‐beneficial microbes regardless of the inoculation site. We, therefore, conclude that PGPB can promote plant growth both directly and indirectly through the interaction between the rhizosphere and phyllosphere, leading to the enrichment of beneficial microorganisms.

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Section: Discussionmentioning

confidence: 99%

Bacillus velezensisSQR9 promotes plant growth through colonization and rhizosphere–phyllosphere bacteria interaction

Yu,

Wang,

Zhang

et al. 2024

Environ Microbiol Rep

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“…Under these conditions, inoculation with the bacterial strains maintained their advantage over the controls through stronger growth and healthier leaves until the end of the vegetation period (Figure 4). The effects of inoculation with A4130 and D4P002 were significant across all three years of the plant trial [19]. Inoculation with the strain C2P003 also resulted in a distinct effect on the plant health of the ash seedlings, but at two time points this effect was not significant (Figure 4).…”

Section: Inoculation Of Ash Seedlingsmentioning

confidence: 95%

“…Cells of the A. altamirensis strain C2P003 were cultured in R2 broth for two days at 25 • C and washed two times with 0.3% NaCl. Genomic DNA was extracted by the Genomic-Tip 20 Kit (Qiagen, Hilden, Germany) as described previously [19]. The fragment size, quantity and quality of DNA were assessed on a 1% agarose gel and with a NanoDrop ND-1000 spectrophotometer (Thermo Scientific, Waltham, MA, USA).…”

Section: Genome Sequencingmentioning

confidence: 99%

“…A total of 120 bacterial core marker genes were used to form a concatenated amino acid sequence alignment, which was used to calculate a maximum-likelihood tree (LG substitution model with F+G+I) with MEGA. The calculation of the ANI and dDDH values between C2P003 and the type strain of A. altamirensis DSM 21988 was performed as described previously [19].…”

Section: Phylogenetic and Genome Analysesmentioning

confidence: 99%

“…With the exception of Aureimonas, all these genera are known for strains with plant-beneficial traits. Thus, a health-stabilizing effect on ash seedlings was recently demonstrated for a Luteimonas fraxinea strain obtained from the leaves of healthy ashes [19]. Other members of Luteimonas also had stimulating effects based on disease suppression and nitrogen acquisition [20][21][22].…”

Section: Introductionmentioning

confidence: 99%

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Genomic Characterization of Aureimonas altamirensis C2P003—A Specific Member of the Microbiome of Fraxinus excelsior Trees Tolerant to Ash Dieback

Becker

Ulrich

Behrendt

et al. 2022

Plants

Self Cite

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Some European ash trees show tolerance towards dieback caused by the invasive pathogen Hymenoscyphus fraxineus. The microbiome of these trees harbours a range of specific bacterial groups. One of these groups belonging to the species Aureimonas altamirensis was studied in detail by genome analysis and a plant inoculation trial. The strain group was shown to be phylogenetically distinct from clinical isolates by 16S rRNA analysis and phylogenomics. Genome analysis of a representative strain C2P003 resulted in a large number of unique gene sequences in comparison to other well-studied strains of the species. A functional analysis of the genome revealed features associated with the synthesis of exopolysaccharides, protein secretion and biofilm production as well as genes for stress adaptation, suggesting the ability of C2P003 to effectively colonize ash leaves. The inoculation of ash seedlings with C2P003 showed a significant positive effect on the plant health of the seedlings that were exposed to H. fraxineus infection. This effect was maintained over a period of three years and was accompanied by a significant shift in the bacterial microbiome composition one year after inoculation. Overall, the results indicate that C2P003 may suppress H. fraxineus in or on ash leaves via colonization resistance or indirectly by affecting the microbiome.

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Inoculation of Fraxinus excelsior seedlings with bacterial strains to enhance the tolerance against ash dieback

Burghard,

Wende,

Schneck

et al. 2024

J Plant Dis Prot

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Since ash dieback has been recognized as a major threat to Fraxinus excelsior, multiple lines of research have focused on potential management to mitigate this disease. One area of focus has been the microbiome of the trees and the application of biocontrol agents (BCAs). In this study, we aimed to find suitable BCAs that can help control Hymenoscyphus fraxineus and the resulting ash dieback. Therefore, we studied the health of ash seedlings under nursery conditions via inoculation with beneficial bacterial isolates and consortia to suppress the pathogen. By using offspring of two tolerant ash trees, we aimed to optimize this via synergistic effects. We found that Schauerella fraxinea B3P038 and Luteimonas fraxinea D4P002 lowered the symptoms of ash dieback. Using strain-specific qPCR assays, only the persistence of Aureimonas altamirensis C2P003 could be proven in the inoculated ash seedlings. Similarly, the fungal pathogen was also unable to infect the plants during the summer months. In addition, we were able to show that the inoculated bacterial strains influenced the microbiome, even without persisting on the plants. These results are encouraging, as positive effects can be taken from the greenhouse to the field and further studies should follow up on the B3P038, C2P003 and D4P002 strains. However, under field conditions, a strong variation in the effects must be considered. This strongly suggests that in addition to plant scoring, monitoring inoculation success as well as pathogen abundance is necessary.

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Physiological and genomic characterisation of Luteimonas fraxinea sp. nov., a bacterial species associated with trees tolerant to ash dieback

Cited by 13 publications

References 105 publications

Bacillus velezensisSQR9 promotes plant growth through colonization and rhizosphere–phyllosphere bacteria interaction

Bacillus velezensisSQR9 promotes plant growth through colonization and rhizosphere–phyllosphere bacteria interaction

Genomic Characterization of Aureimonas altamirensis C2P003—A Specific Member of the Microbiome of Fraxinus excelsior Trees Tolerant to Ash Dieback

Inoculation of Fraxinus excelsior seedlings with bacterial strains to enhance the tolerance against ash dieback

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