Physiological and growth changes in micropropagated Citrus macrophylla explants due to salinity

Pérez‐Tornero, O.; Tallón, Carlos I.; Porras, I.; Navarro, Josefa M.

doi:10.1016/j.jplph.2009.06.009

Cited by 42 publications

(29 citation statements)

References 38 publications

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“…In the treatments above 150 mM NaCl (continuous exposure) and those above 50 mM NaCl (gradual exposure), Na concentration reached excessive or toxic levels (> 0.25%) for citrus leaves. This pattern, the result of increasing NaCl in the medium, is a common and expected response and has been reported for many citrus species (DIONISIO and ANTONII 1997, SHIYAB et al 2003, PEREZ-TORNERO et al 2009, GHALEB et al 2010. Moreover, it is well documented that tissue concentrations of Cl in Citrus increase in response to NaCl treatments (DIONISIO and ANTONII 1997, PEREZ-TORNERO et al 2009, GHA-LEB et al 2010.…”

Section: Resultssupporting

confidence: 58%

“…This pattern, the result of increasing NaCl in the medium, is a common and expected response and has been reported for many citrus species (DIONISIO and ANTONII 1997, SHIYAB et al 2003, PEREZ-TORNERO et al 2009, GHALEB et al 2010. Moreover, it is well documented that tissue concentrations of Cl in Citrus increase in response to NaCl treatments (DIONISIO and ANTONII 1997, PEREZ-TORNERO et al 2009, GHA-LEB et al 2010. The concentration of chloride in explants was several times higher than that of Na, and a similar response was also observed for jojoba (Simmondsia chinensis (Link) Schneid) explants under high salinity conditions (ROUSSOS et al 2007).…”

Section: Resultssupporting

confidence: 58%

“…1). Similarly, in explants of Citrus macrophylla a significant decrease in the shoot number was observed at 30 mM NaCl (PEREZ-TORNERO et al 2009). Also, it was reported that continuous or gradual exposure to high NaCl in nutrient medium led to a reduced number of shoots in sour orange (SHIYAB et al 2003).…”

Section: Resultsmentioning

confidence: 90%

“…It is well established that salinity stress is followed by chlorophyll degradation that leads to a chlorotic appearance in explants. High concentrations of NaCl applied under in vitro conditions reduced the chlorophyll content in Citrus (PEREZ-TORNERO et al 2009) and other woody species (CHATZISSAVVIDIS et al 2008). Similarly, GHALEB et al (2010) experimenting with sour orange cultivated under in vitro conditions recorded complete leaf damage at 300 mM NaCl.…”

Section: Resultsmentioning

confidence: 94%

“…Field screening in saline sites to select salt tolerant genotypes of citrus rootstocks is complicated because of differential ontogenic reactions of the plant to salinity and a large genotype in combination with environment interaction. In vitro tissue culture is a simple technique that offers a suitable alternative for the study of physiological mechanisms of tolerance to salinity, since it provides relatively fast responses, needs a short testing time and a controlled environment, especially in tree species that have long reproductive cycles (PEREZ-TORNERO et al 2009). …”

Section: Introductionmentioning

confidence: 99%

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Responses of trifoliate orange (poncirus trifoliata (l.) raf.) to continuously and gradually increasing nacl concentration

Chatzissavvidis¹,

Antonopoulou²,

Therios³

et al. 2014

Acta Botanica Croatica

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-The effect of continuous or gradual stress due to NaCl on in vitro growth, proline and sugar accumulation and nutrient acquisition of trifoliate orange (Poncirus trifoliata (L.) Raf.) explants was studied. Apical shoot tips obtained from previous subculture were transferred to a Murashige and Skoog nutrient medium for proliferation and were exposed to continuous or gradual salinity stress for 42 days. The salt used to induce salinity was NaCl added in six concentrations: 0, 50, 100, 150, 200 and 300 mM. Gradual salinization was achieved by transferring the explants sequentially every week to the above mentioned NaCl concentrations. Most salt treatments had a negative effect on the growth parameters of explants. Sodium concentration of explants increased in all NaCl treatments compared to control and it was higher in the treatments with gradual exposure to salinity. Potassium concentration was reduced, mostly in the treatments with continuous exposure. Calcium and Mg concentrations increased in all saline treatments. In general, the high salinity level in the substrate enhanced the proline and sugar concentrations of the studied explants. In conclusion, salinity had significant impacts on the growth and chemical status of P. trifoliata.

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