Physiological and immunological conditions of wild populations of Farfantepenaeus duorarum from the campeche sound (Crustacea, Penaeidae)

Rosas, Carlos; Cooper, Edwin L.; Pascual, Cristina; Brito, Roberto; Gelabert, R; Moreno, T.; Sánchez, Ariadna

doi:10.1007/s00227-007-0743-8

Cited by 10 publications

(14 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The pH of the reaction system for PO activity assay in shrimp is inconsistent in different studies, including 6 [10], 6.4 [4], 7 [13] and 7.6 [11]. In the present study, no optimal pH can be found between 5.8 and 7.8 since PO activity increases continually and significantly with the increase of pH (P < 0.05) (Fig.…”

Section: Effects Of Reaction Conditionscontrasting

confidence: 69%

“…All these factors, if not in a proper range, can affect the accurate determination of PO activity. Moreover, the inhibition effect of EDTA on PO activity [12] is most probably ignored since EDTA anticoagulant has still been used for separating plasma samples in recent studies [4,5,9,13]. Furthermore, we noticed that the assayed PO activity in the same preserved plasma could obviously change when it was assayed at different times.…”

Section: Introductionmentioning

confidence: 89%

“…Several other studies in which PO activity is detected by artificial induction of plasma have mistakenly treated the proenzyme in plasma as proPO [13,18]. Actually, artificial induction of plasma can result in the activation of all hemocyanin polymers to show PO activity.…”

Section: Origin Of Po Activity In Plasmamentioning

confidence: 96%

“…The final concentration of L-DOPA in reaction system for PO activity assay contains a wide range in previous studies, such as 0.06 [4], 0.75 [19], 1 [13], 1.5 [20], 2.4 [21] and 2.85 [22] mg ml À1 . In this study, however, the assayed PO activity is significantly lower when the final concentration of L-DOPA is 1 mg ml À1 (P < 0.05) (Fig.…”

Section: Effects Of Reaction Conditionsmentioning

confidence: 99%

See 3 more Smart Citations

Reconsideration of phenoloxidase activity determination in white shrimp Litopenaeus vannamei

Huang

Yang

Wang

2010

Fish & Shellfish Immunology

View full text Add to dashboard Cite

Section: Effects Of Reaction Conditionscontrasting

confidence: 69%

Section: Introductionmentioning

confidence: 89%

Section: Origin Of Po Activity In Plasmamentioning

confidence: 96%

Section: Effects Of Reaction Conditionsmentioning

confidence: 99%

See 2 more Smart Citations

Reconsideration of phenoloxidase activity determination in white shrimp Litopenaeus vannamei

Huang

Yang

Wang

2010

Fish & Shellfish Immunology

View full text Add to dashboard Cite

“…Lobsters were held and kept unfed for 2 d prior to analyses. Before extracting hemolymph for laboratory analyses, lobsters were placed in aerated seawater 5°C lower than the temperature of the water of the holding tanks to reduce their metabolic activity and to minimize handling effects on hemolymph components (Rosas et al 2007). …”

Section: Lobster Samplingmentioning

confidence: 99%

Physiological and immunological characterization of Caribbean spiny lobsters Panulirus argus naturally infected with Panulirus argus Virus 1 (PaV1)

Pascual-Jímenez¹,

Huchin-Mian²,

Simões³

et al. 2012

Dis. Aquat. Org.

View full text Add to dashboard Cite

The present study compares 13 physiological and immunological variables between a group of healthy Panulirus argus lobsters and a group of lobsters naturally infected with Panulirus argus Virus 1 (PaV1). Viral infection was determined through histopathology and PCR. Ten of the 13 variables differed significantly between the 2 groups. Using these variables, a principal component analysis yielded 2 separate clusters: one corresponding to the healthy group and the other corresponding to the infected group. In particular, infected lobsters exhibited significantly lower levels of osmotic pressure, total hemocyte counts, plasmatic proteins, and total phenoloxidase (PO) activity in plasma, as well as significantly higher levels of cholesterol and acylglycerides. These features are consistent with metabolic wasting, hyperlipidemia, and presumed immune suppression. Infection with PaV1 appears to increase the susceptibility of lobsters to some other opportunistic pathogens, as 61.1% of infected lobsters presented infestations of ciliate epibionts (Epystilis and Zoothamniun) in the gill chamber compared with 11.5% lobsters in the healthy group. Infected lobsters also showed significantly higher levels of total PO activity in degranulated hemocytes and trypsin inhibitor activity, potentially indicating activation of immune response by the PO system during the systemic infection with PaV1.

show abstract

Reproductive performance, biochemical composition and fatty acid profile of wild-caught and 2nd generation domesticated Farfantepenaeus duorarum (Burkenroad, 1939) broodstock

Emerenciano¹,

Cuzon²,

Mascaró³

et al. 2012

Aquaculture

View full text Add to dashboard Cite

A 30-day trial was performed to evaluate the reproductive performance of wild and 2nd generation domesticated Farfantepenaeus duorarum broodstock. Changes in biochemical composition and fatty acids (FA) profile in the 1st and 4th spawn order females were used as indicators of nutritional condition. Wild population of F. duorarum presented significantly better reproductive outcomes as compared to domesticated ones. Wild spawners achieved significantly higher number of eggs per spawn, number of egg per spawn per g of spawner's body weight and number of nauplii per spawn (P < 0.05). Latency period was significantly shorter (P0.05) for wild than for domesticated females (11 vs 17 days). Wild spawners also presented less mortality (6 vs 12%), higher number of total spawns (51 vs 33), spawns per ablated and per spawning females as well as higher total eggs and nauplii production. On the other hand, domesticated females presented higher number of females that spawned at least once (89 vs 76%). The higher spawning activity was reflected in lower levels of acylglycerides (AG) and cholesterol content in wild hepatopancreas (HP) and ovary (OV) (Pb0.05). FA profile, mainly represented by ARA, EPA and sum of n−3 were higher in hepatopancreas and ovary of wild females, however lower levels were detected in eggs as a result of a high spawning activity. Multivariate analysis performed to detected simultaneous variation in biochemical variables indicated AG as variation pattern's responsive. Highlights ► Original paper comparing the reproductive performance of wild and G2 F. duorarum. ► Interesting findings using biochemical composition/FA profile as a nutritional index. ► Multivariate analysis method contributed to explain the results. ► Wild population performed better reproductive performance.

show abstract

Physiological and immunological conditions of wild populations of Farfantepenaeus duorarum from the campeche sound (Crustacea, Penaeidae)

Cited by 10 publications

References 30 publications

Reconsideration of phenoloxidase activity determination in white shrimp Litopenaeus vannamei

Reconsideration of phenoloxidase activity determination in white shrimp Litopenaeus vannamei

Physiological and immunological characterization of Caribbean spiny lobsters Panulirus argus naturally infected with Panulirus argus Virus 1 (PaV1)

Reproductive performance, biochemical composition and fatty acid profile of wild-caught and 2nd generation domesticated Farfantepenaeus duorarum (Burkenroad, 1939) broodstock

Contact Info

Product

Resources

About