Physiological and Metabolic Responses of Leymus chinensis Seedlings to Alkali Stress

Yan, Ge; Shi, Yujie; Chen, Fangfang; Mu, Changkao; Wang, Junfeng

doi:10.3390/plants11111494

Cited by 7 publications

(3 citation statements)

References 26 publications

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“…Its wide adaptability and economic value make it an ideal species for the restoration of alkaline lands in Northeast China. Although several studies have focused on physiological characteristics, metabolism and transcriptional changes of L. chinensis under alkali stress ( Jin et al., 2008 ; Yan et al., 2022 , 2023 ), most of them are descriptive. There is a lack of research on the metabolic regulation of different organs and the root secretions of L. chinensis grown under alkali-stress conditions.…”

Section: Introductionmentioning

confidence: 99%

Carboxylic acid accumulation and secretion contribute to the alkali-stress tolerance of halophyte Leymus chinensis

Wang,

Zhao,

Sun

et al. 2024

Front. Plant Sci.

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Leymus chinensis is a dominant halophytic grass in alkalized grasslands of Northeast China. To explore the alkali-tolerance mechanism of L. chinensis, we applied a widely targeted metabolomic approach to analyze metabolic responses of its root exudates, root tissues and leaves under alkali-stress conditions. L. chinensis extensively secreted organic acids, phenolic acids, free fatty acids and other substances having -COOH or phosphate groups when grown under alkali-stress conditions. The buffering capacity of these secreted substances promoted pH regulation in the rhizosphere during responses to alkali stress. L. chinensis leaves exhibited enhanced accumulations of free fatty acids, lipids, amino acids, organic acids, phenolic acids and alkaloids, which play important roles in maintaining cell membrane stability, regulating osmotic pressure and providing substrates for the alkali-stress responses of roots. The accumulations of numerous flavonoids, saccharides and alcohols were extensively enhanced in the roots of L. chinensis, but rarely enhanced in the leaves, under alkali-stress conditions. Enhanced accumulations of flavonoids, saccharides and alcohols increased the removal of reactive oxygen species and alleviated oxygen damage caused by alkali stress. In this study, we revealed the metabolic response mechanisms of L. chinensis under alkali-stress conditions, emphasizing important roles for the accumulation and secretion of organic acids, amino acids, fatty acids and other substances in alkali tolerance.

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Section: Introductionmentioning

confidence: 99%

Carboxylic acid accumulation and secretion contribute to the alkali-stress tolerance of halophyte Leymus chinensis

Wang,

Zhao,

Sun

et al. 2024

Front. Plant Sci.

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“…According to Zhao et al 10 , plants can adapt to alkaline environments by thickening their epidermis and flesh, shrinking their leaves, and altering their tissue structure. The high pH of alkaline stress disrupts plant ion homeostasis, hinders photosynthesis and photosynthetic pigment production, damages biomolecules such as DNA, lipids and proteins, and causes slow growth and even death of plants 11 . Currently, studies on the physiological aspects of stress tolerance mainly focus on the physiological characteristics of pests and diseases, heavy metals, salinity, and water 12 .…”

Section: Introductionmentioning

confidence: 99%

Responses of growth and photosynthesis to alkaline stress in three willow species

Qiao,

Ma,

et al. 2024

Sci Rep

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Investigating differences in resistance to alkaline stress among three willow species can provide a theoretical basis for planting willow in saline soils. Therefore we tested three willow species (Salix matsudana, Salix gordejevii and Salix linearistipularis), already known for their high stress tolerance, to alkaline stress environment at different pH values under hydroponics. Root and leaf dry weight, root water content, leaf water content, chlorophyll content, photosynthesis and chlorophyll fluorescence of three willow cuttings were monitored six times over 15 days under alkaline stress. With the increase in alkaline stress, the water retention capacity of leaves of the three species of willow cuttings was as follows: S. matsudana > S. gordejevii > S. linearistipularis and the water retention capacity of the root system was as follows: S. gordejevii > S. linearistipularis > S. matsudana. The chlorophyll content was significantly reduced, damage symptoms were apparent. The net photosynthetic rate (Pn), rate of transpiration (E), and stomatal conductance (Gs) of the leaves showed a general trend of decreasing, and the intercellular CO2 concentration (Ci) of S. matsudana and S. gordejevii first declined and then tended to level off, while the intercellular CO2 concentration of S. linearistipularis first declined and then increased. The quantum yield and energy allocation ratio of the leaf photosystem II (PSII) reaction centre changed significantly (φPo, Ψo and φEo were obviously suppressed and φDo was promoted). The photosystem II (PSII) reaction centre quantum performance index and driving force showed a clear downwards trend. Based on the results it can be concluded that alkaline stress tolerance of three willow was as follows: S. matsudana > S. gordejevii > S. linearistipularis. However, since the experiment was done on young seedlings, further study at saplings stage is required to revalidate the results.

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“…Similarly, it increases the the content of proline, soluble sugar, and polyol (sorbitol) in wheat (Guo et al 2015). Moreover, the salt-tolerant perennial rhizome plant Leymus chinensis exhibit a higher concentration of nucleotides, amino acids, and organic acids, and a lower concentration of soluble sugars against alkali stress (Yan et al 2022). The changes in the types and contents of these metabolites are generally considered necessary for plants to cope with alkali stress.…”

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confidence: 99%

Transcriptome and Metabolome Integrated Analysis Reveals the Mechanism of Cinnamomum bodinieri Root Response to Alkali Stress

Han¹,

Zhang²,

Li³

et al. 2023

Plant Mol Biol Rep

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Cinnamomun bodinieri's normal growth and development are hampered by alkali stress, impeding its production and application of Cinnamomum bodinieri. The root organs being in direct contact with the cultivation environment, are sensitive to environmental stress. The present study revealed the differentially expressed genes and differentially metabolized products of Cinnamomum bodinieri root under alkali stress employing transcriptome and metabonomic analysis. The ndings revealed that 690 differentially expressed genes and 269 metabolites were signi cantly different among HT6 and HCK6. Similarly, 1000 differentially expressed genes and 360 metabolites with signi cant differences were identi ed in HT48.vs.HCK48 combination. The combined analysis of transcriptome and metabolome identi ed 9 metabolic pathways at 6h and 48h after alkali treatment, including the biosynthesis pathway of tropane, piperidine and pyridine alkaloids, pyrimidine metabolic pathway, phenylalanine metabolic pathway, isoquinoline alkaloid biosynthesis pathway, glycolysis/gluconeogenesis pathway, avonoid biosynthesis pathway, fatty acid biosynthesis pathway, carbon xation pathway in photosynthetic organisms, the metabolic pathway of amino sugar and nucleotide sugar. Therefore, the strategy of Cinnamomum bodinieri to cope with alkali stress may be to increase osmotic regulation and antioxidant activity by accumulating alkaloids, avonoids secondary metabolites, and N-acetyl-L-phenylalanine, ensure the stability of cell structure and function through the accumulation of lauric acid and palmitic acid, provide energy for plants to withstand alkali stress by accelerating the glycolysis process, and improve plants' resistance to biological and abiotic stress by inducing the activity of chitinase, The accumulation of oxaloacetic acid and other organic acids alleviates alkali stress environment. This study provides support for the analysis of the pathways and regulatory networks of Cinnamomum bodinieri in response to alkali stress.

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Physiological and Metabolic Responses of Leymus chinensis Seedlings to Alkali Stress

Cited by 7 publications

References 26 publications

Carboxylic acid accumulation and secretion contribute to the alkali-stress tolerance of halophyte Leymus chinensis

Carboxylic acid accumulation and secretion contribute to the alkali-stress tolerance of halophyte Leymus chinensis

Responses of growth and photosynthesis to alkaline stress in three willow species

Transcriptome and Metabolome Integrated Analysis Reveals the Mechanism of Cinnamomum bodinieri Root Response to Alkali Stress

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