Physiological and metabolic traits of Taxol biosynthesis of endophytic fungi inhabiting plants: Plant-microbial crosstalk, and epigenetic regulators

Mohamed, Nabil Z.; Shaban, Lamis; Safan, Samia; Elsayed, Abdelrahman H.

doi:10.1016/j.micres.2023.127385

Cited by 5 publications

(3 citation statements)

References 332 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These proteins may be involved in chromatin remodeling and the regulation of gene expression, including those related to fungal Taxol biosynthesis (El-Sayed et al 2021 ). The increased expression of specific proteins suggests that the chromatin structure in fungus is being altered, which can lead to changes in the accessibility of gene clusters and the activation of silent Taxol biosynthetic pathways (Mohamed et al 2023 ). Moreover, as a result of various elicitations and stress factors, fungal Taxol production was significantly increased at least in some fungi (Soliman et al 2013 ).…”

Section: Induction Of Elicitorsmentioning

confidence: 99%

“…The main issue limiting Taxol biosynthesis is long storage and frequent subculturing of fungi. In this context of restoring and enhancing the Taxol biosynthetic potency by epigenetic regulating mechanisms such as epigenetic regulators, transcriptional factors, metabolic manipulators, microbial communications, and microbial cross-talking in endophytic fungi is of great relevance (Mohamed et al 2023 ). Using reverse transcription-quantitative polymerase chain reaction (RT-qPCR), the expression of Taxol biosynthesis rate-limiting genes, such as ts , dbat , and bapt , was confirmed in an Aspergillus isolate with influx of acetyl-CoA (El-Sayed et al 2019b ).…”

Section: Precursor Feedingmentioning

confidence: 99%

“…However, the successful transfer of the entire taxane biosynthetic pathway to these organisms has not been achieved yet. According to Nazhand et al ( 2020 ) and Mohamed et al ( 2023 ) Taxol and its derivatives are worth over $1 billion per year.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Insights into Taxol® biosynthesis by endophytic fungi

Subban,

Kempken

2023

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

There have been two hundred reports that endophytic fungi produce Taxol®, but its production yield is often rather low. Although considerable efforts have been made to increase Taxol/taxanes production in fungi by manipulating cocultures, mutagenesis, genome shuffles, and gene overexpression, little is known about the molecular signatures of Taxol biosynthesis and its regulation. It is known that some fungi have orthologs of the Taxol biosynthetic pathway, but the overall architecture of this pathway is unknown. A biosynthetic putative gene homology approach, combined with genomics and transcriptomics analysis, revealed that a few genes for metabolite residues may be located on dispensable chromosomes. This review explores a number of crucial topics (i) finding biosynthetic pathway genes using precursors, elicitors, and inhibitors; (ii) orthologs of the Taxol biosynthetic pathway for rate-limiting genes/enzymes; and (iii) genomics and transcriptomics can be used to accurately predict biosynthetic putative genes and regulators. This provides promising targets for future genetic engineering approaches to produce fungal Taxol and precursors. Key points • A recent trend in predicting Taxol biosynthetic pathway from endophytic fungi. • Understanding the Taxol biosynthetic pathway and related enzymes in fungi. • The genetic evidence and formation of taxane from endophytic fungi.

show abstract

Section: Induction Of Elicitorsmentioning

confidence: 99%

Section: Precursor Feedingmentioning

confidence: 99%