Physiological and molecular adaptations to drought in Andean potato genotypes

Vásquez-Robinet, Cecilia; Mane, Shrinivasrao P.; Ulanov, Alexander; Watkinson, Jonathan I.; Stromberg, Verlyn K.; Koeyer, David De; Schafleitner, Roland; Willmot, David B.; Bonierbale, Merideth; Bohnert, Hans J.; Grene, Ruth

doi:10.1093/jxb/ern073

Cited by 188 publications

(146 citation statements)

References 62 publications

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“…The molecular signal transduction that starts in the roots eventually is reflected in physiological responses in the plant as a whole. For example, ABA biosynthesis in roots triggers defense responses in leaves, regulating a series of physiological responses based on altered hormone metabolism and subsequent alteration in expression of transcripts and in activation of proteins (Vasquez-Robinet et al, 2008).…”

Section: Gm18srrna Gmdreb1amentioning

confidence: 99%

Soybean physiology and gene expression during drought

Stolf-Moreira¹,

Medri²,

Neumaier³

et al. 2010

Genet. Mol. Res.

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ABSTRACT. Soybean genotypes MG/BR46 (Conquista) and BR16, drought-tolerant and -sensitive, respectively, were compared in terms of morphophysiological and gene-expression responses to water stress during two stages of development. Gene-expression analysis showed differential responses in Gmdreb1a and Gmpip1b mRNA expression within 30 days of water-deficit initiation in MG/BR46 (Conquista) plants. Within 45 days of initiating stress, Gmp5cs and Gmpip1b had relatively higher expression. Initially, BR16 showed increased expression only for Gmdreb1a, and later (45 days) for Gmp5cs, Gmdefensin and Gmpip1b. Only BR16 presented down-regulated expression of genes, such as Gmp5cs and Gmpip1b, 30 days after the onset of moisture stress, and Gmgols after 45 days of stress. The faster perception of water stress in MG/BR46 (Conquista) and the better maintenance of up-regulated gene expression than in the sensitive BR16 genotype imply mechanisms by which the former is better adapted to tolerate moisture deficiency.

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Section: Gm18srrna Gmdreb1amentioning

confidence: 99%

Soybean physiology and gene expression during drought

Stolf-Moreira¹,

Medri²,

Neumaier³

et al. 2010

Genet. Mol. Res.

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“…Potato is often considered to be a drought sensitive crop through its all developmental stages (Schafleitner et al 2007), and regularly suffers drought stress in most growing regions (Vasquez-Robinet et al 2008). In contrast, potato has been classified as moderately salt-sensitive, and losses in potato production due to salinity in its planting areas are currently expected (Queirós et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Transgenic potato overexpressing Arabidopsis cytosolic AtDHAR1 showed higher tolerance to herbicide, drought and salt stresses

et al. 2011

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Ascorbate (vitamin C) is a powerful antioxidant and scavenger of free radicals that protects plants against oxidative damage caused by adverse environmental conditions such as drought, salt stress, and herbicide use. Dehydroascorbate reductase (DHAR; EC 1.8.5.1) and monodehydroascorbate reductase (MDAR; EC 1.6.5.4) are crucial for ascorbate regeneration and the maintenance of a pool of the reduced form. In this study, we report the development of transgenic potato (Solanum tuberosum L.) that overexpresses the Arabidopsis thaliana DHAR gene (AtDHAR1) in the cytosol. The transgenic plants exhibited up to 4.5 times the DHAR activity and up to 2.8 times the level of reduced ascorbate found in the wild-type plants. When subjected to methylviologen treatment, the transgenic plants exhibited enhanced tolerance in terms of less ion leakage, greater chlorophyll contents, less accumulation of hydrogen peroxide, and less severe visual injury symptoms. Moreover, the transgenic plants exhibited faster growth under drought and salt stress. Our results demonstrate that elevating ascorbate contents by overproducing DHAR represents a viable approach for the development of herbicide-tolerant potato.Key Words: ascorbate, potato, vitamin C, dehydroascorbate, methylviologen. IntroductionAdverse environmental conditions such as drought, salt stress, and use of herbicide promote the overproduction of reactive oxygen species (ROS) in plant cells. ROS such as singlet oxygen (O 2 1 ), superoxide radicals (O 2 ⋅), hydrogen peroxide (H 2 O 2 ), and hydroxyl radicals (OH⋅) are believed to be the major factor responsible for rapid cellular damage due to their high reactivity with membrane lipids, proteins, and DNA (Asada and Takahashi 1987, Hung et al. 2005, Mittler 2002). Scavenging and reducing the deleterious effects of ROS is performed by several enzymatic and nonenzymatic systems in plant cells. A network of low-molecularweight antioxidants, such as ascorbate (AsA), glutathione (GSH), and α-tocopherol, and antioxidative enzymes such as superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT) are crucial for preventing and controlling the dangerous affects of ROS (Noctor and Foyer 1998). Specifically, enzymes involved in catalyzing redox reactions and in maintaining pools of reduced AsA and GSH in the AsA-GSH pathway, mainly monodehydroascorbate reductase (MDAR; EC 1.6.5.4), dehydroascorbate reductase (DHAR; EC 1.8.5.1), and glutathione reductase, are considered of paramount importance in plant antioxidant defense mechanisms.The antioxidant AsA can directly scavenge free radicals (Halliwell and Gutteridge 2000), and is particularly important as an electron donor for detoxification of H 2 O 2 via ascorbate peroxidase in plant cells (Noctor and Foyer 1998). Moreover, AsA is a major redox buffer in plants , a cofactor of many enzymes (Smirnoff and Wheeler 2000), a regulator of cell division and growth (Kerk and Feldman 1995), and a molecule used in signal transduction in plants (Noctor et al. 2000). AsA is synthesized in t...

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“…Potato is relatively vulnerable to abiotic stresses such as drought and high salinity and is classified as an environmentally sensitive crop along with paddy rice and sugarcane (Saccharum officinarum; Levy, 1985;Bray et al, 2000;Vasquez-Robinet et al, 2008). Potato tuber initiation, bulking, and tuber growth stage especially are sensitive to drought stress (Evers et al, 2010).…”

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confidence: 99%

Expression of StMYB1R-1, a Novel Potato Single MYB-Like Domain Transcription Factor, Increases Drought Tolerance

et al. 2010

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Potato (Solanum tuberosum) is relatively vulnerable to abiotic stress conditions such as drought, but the tolerance mechanisms for such stresses in potato are largely unknown. To identify stress-related factors in potato, we previously carried out a genetic screen of potato plants exposed to abiotic environmental stress conditions using reverse northern-blot analysis. A cDNA encoding a putative R1-type MYB-like transcription factor (StMYB1R-1) was identified as a putative stress-response gene. Here, the transcript levels of StMYB1R-1 were enhanced in response to several environmental stresses in addition to drought but were unaffected by biotic stresses. The results of intracellular targeting and quadruple 9-mer protein-binding microarray analysis indicated that StMYB1R-1 localizes to the nucleus and binds to the DNA sequence G / A GATAA. Overexpression of a StMYB1R-1 transgene in potato plants improved plant tolerance to drought stress while having no significant effects on other agricultural traits. Transgenic plants exhibited reduced rates of water loss and more rapid stomatal closing than wild-type plants under drought stress conditions. In addition, overexpression of StMYB1R-1 enhanced the expression of droughtregulated genes such as AtHB-7, RD28, ALDH22a1, and ERD1-like. Thus, the expression of StMYB1R-1 in potato enhanced drought tolerance via regulation of water loss. These results indicated that StMYB1R-1 functions as a transcription factor involved in the activation of drought-related genes.

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Physiological and molecular adaptations to drought in Andean potato genotypes

Cited by 188 publications

References 62 publications

Soybean physiology and gene expression during drought

Soybean physiology and gene expression during drought

Transgenic potato overexpressing Arabidopsis cytosolic AtDHAR1 showed higher tolerance to herbicide, drought and salt stresses

Expression of StMYB1R-1, a Novel Potato Single MYB-Like Domain Transcription Factor, Increases Drought Tolerance

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