Physiological and molecular analyses of black and yellow seeded Brassica napus regulated by 5-aminolivulinic acid under chromium stress

Gill, Rafaqat Ali; Ali, Basharat; Islam, Faisal; Farooq, Muhammad Ahsan; Gill, Muhammad Bilal; Mwamba, Theodore M.; Zhou, Weijun

doi:10.1016/j.plaphy.2015.06.001

Cited by 103 publications

(49 citation statements)

References 72 publications

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“…From the literature, it is well documented that HSPs act as metal stress protectants and scavenges the negative effects on plants [51, 52]. Recently, Gill et al [25] confirmed that HSP-90 level was increased under the Cr stress that was in the same line of our RNA sequence analyses results. Previous studies showed that ABA receptors PYRAB-actin resistance1/ PYR-like (PYR1/PYL) draw out the stress respond inside the plant body [53].…”

Section: Discussionsupporting

confidence: 89%

“…Cr contents were estimated according to Gill et al [25] and for GSH contents the methodology of Law et al [27] was followed with some modifications [25]. …”

Section: Methodsmentioning

confidence: 99%

“…In order to verify the Hiseq 2000™ data both RNA and RNA-Seq samples were used, and we followed the previous methodology of Gill et al [25].

Fig.…”

Section: Methodsmentioning

confidence: 99%

“…As for oilseed rape ( Brassica napus L.) plants, recent studies have concentrated its capability to respond the heavy metal stress, uptake and distribution of metal contents, effect on photosynthetic gas exchange capacity, biochemical and ultrastructural changes [7, 24, 25]. According to our knowledge there is no report which reveals the DGE and TPA analyses under the Cr stress mitigated by GSH.…”

Section: Introductionmentioning

confidence: 99%

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Comparative transcriptome profiling of two Brassica napus cultivars under chromium toxicity and its alleviation by reduced glutathione

et al. 2016

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BackgroundChromium (Cr) being multifarious industrial used element, is considered a potential environmental threat. Cr found to be a prospective water and soil pollutant, and thus it is a current area of concern. Oilseed rape (Brassica napus L.) is well known as a major source of edible oil around the globe. Due to its higher growth, larger biomass and capability to uptake toxic materials B. napus is considered a potential candidate plant against unfavorable conditions. To date, no study has been done that described the Cr and GSH mechanism at RNA-Seq level.ResultsBoth digital gene expression (DGE) and transcriptome profile analysis (TPA) approaches had opened new insights to uncover the several number of genes related to Cr stress and GSH alleviating mechanism in two leading cultivars (ZS 758 and Zheda 622) of B. napus plants. Data showed that Cr inhibited KEGG pathways i.e. stilbenoid, diarlyheptanoid and gingerol biosynthesis; limonene and pentose degradation and glutathione metabolism in ZS 758; and ribosome and glucosinolate biosynthesis in Zheda-622. On the other hand, vitamin B6, tryptophan, sulfur, nitrogen and fructose and manose metabolisms were induced in ZS 758, and zeatin biosynthesis, linoleic acid metabolism, arginine and proline metabolism, and alanine, asparate and glutamate metabolism pathways in Zheda 622. Cr increased the TFs that were related to hydralase activity, antioxidant activity, catalytic activity phosphatase and pyrophosphatase activity in ZS 758, and vitamin binding and oxidoreductase activity in Zheda 622. Cr also up-regulated the promising proteins related to intracellular membrane bounded organelles, nitrile hyrdatase activity, cytoskeleton protein binding and stress response. It also uncovered, a novel Cr-responsive protein (CL2535.Contig1_All) that was statistically increased as compared to control and GSH treated plants. Exogenously applied GSH successfully not only recovered the changes in metabolic pathways but also induced cysteine and methionine metabolism in ZS 758 and ubiquinone and other terpenoid-quinone biosynthesis pathways in Zheda 622. Furthermore, GSH increased the level of TFs i.e. the gene expression of antioxidant and catalytic activities, iron ion binding and hydrolase activity as compared with Cr. Moreover, results pointed out a novel GSH responsive protein (CL827.Contig3_All) whose expression was found to be significantly increased when compared than Cr stress. Results further delineated that GSH induced TFs such as glutathione disulphide oxidoreducatse and aminoacyl-tRNA ligase activity, and beta glucosidase activity in ZS 758. Similarly in Zheda 622, GSH induced the TFs for instance DNA binding and protein dimerization activity. GSH also highlighted the proteins that were involved in transportation, photosynthesis process, RNA polymerase activity, and against the metal toxicity. These results indicated that cultivar ZS 758 had better metabolism and showed higher tolerance against Cr toxicity.ConclusionThe responses of ZS 758 and Zheda 622 differed consider...

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Section: Discussionsupporting

confidence: 89%

“…Cr contents were estimated according to Gill et al [25] and for GSH contents the methodology of Law et al [27] was followed with some modifications [25]. …”

Section: Methodsmentioning

confidence: 99%

“…In order to verify the Hiseq 2000™ data both RNA and RNA-Seq samples were used, and we followed the previous methodology of Gill et al [25].

Fig.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative transcriptome profiling of two Brassica napus cultivars under chromium toxicity and its alleviation by reduced glutathione

et al. 2016

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“…Inhibition of chlorophyll contents by Ni toxicity in tomato and cotton has been reported (Khaliq et al, 2016; Mosa et al, 2016). Decrease in the chlorophyll content can be attributed to the inhibition of proto chlorophyllide reductase and ALA dehydratase enzymes following Ni contamination, which play crucial roles in chlorophyll biosynthesis (Noriega et al, 2007; Gill et al, 2015). In our study, P. formosus- inoculated plants exhibited significant increases in chlorophyll content as compared to non-inoculated plants under different concentrations of Ni.…”

Section: Discussionmentioning

confidence: 99%

Endophytic Paecilomyces formosus LHL10 Augments Glycine max L. Adaptation to Ni-Contamination through Affecting Endogenous Phytohormones and Oxidative Stress

et al. 2017

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This study investigated the Ni-removal efficiency of phytohormone-producing endophytic fungi Penicillium janthinellum, Paecilomyces formosus, Exophiala sp., and Preussia sp. Among four different endophytes, P. formosus LHL10 was able to tolerate up to 1 mM Ni in contaminated media as compared to copper and cadmium. P. formosus LHL10 was further assessed for its potential to enhance the phytoremediation of Glycine max (soybean) in response to dose-dependent increases in soil Ni (0.5, 1.0, and 5.0 mM). Inoculation with P. formosus LHL10 significantly increased plant biomass and growth attributes as compared to non-inoculated control plants with or without Ni contamination. LHL10 enhanced the translocation of Ni from the root to the shoot as compared to the control. In addition, P. formosus LHL10 modulated the physio-chemical apparatus of soybean plants during Ni-contamination by reducing lipid peroxidation and the accumulation of linolenic acid, glutathione, peroxidase, polyphenol oxidase, catalase, and superoxide dismutase. Stress-responsive phytohormones such as abscisic acid and jasmonic acid were significantly down-regulated in fungal-inoculated soybean plants under Ni stress. LHL10 Ni-remediation potential can be attributed to its phytohormonal synthesis related genetic makeup. RT-PCR analysis showed the expression of indole-3-acetamide hydrolase, aldehyde dehydrogenase for indole-acetic acid and geranylgeranyl-diphosphate synthase, ent-kaurene oxidase (P450-4), C13-oxidase (P450-3) for gibberellins synthesis. In conclusion, the inoculation of P. formosus can significantly improve plant growth in Ni-polluted soils, and assist in improving the phytoremediation abilities of economically important crops.

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Heavy Metal Toxicity: Physiological Implications of Metal Toxicity in Plants

Małkowski

Sitko

Zieleźnik-Rusinowska

et al. 2019

Plant Metallomics and Functional Omics

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Physiological and molecular analyses of black and yellow seeded Brassica napus regulated by 5-aminolivulinic acid under chromium stress

Cited by 103 publications

References 72 publications

Comparative transcriptome profiling of two Brassica napus cultivars under chromium toxicity and its alleviation by reduced glutathione

Comparative transcriptome profiling of two Brassica napus cultivars under chromium toxicity and its alleviation by reduced glutathione

Endophytic Paecilomyces formosus LHL10 Augments Glycine max L. Adaptation to Ni-Contamination through Affecting Endogenous Phytohormones and Oxidative Stress

Heavy Metal Toxicity: Physiological Implications of Metal Toxicity in Plants

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