Physiological and molecular biological characteristics of heterotrophic ammonia oxidation by Bacillus sp. LY

Lin, Yan; Kong, Hainan; Wu, Deyi; Li, Chunjie; Wang, Rongying; Tanaka, Shinpei

doi:10.1007/s11274-010-0336-4

Cited by 27 publications

(5 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most investigations on nitrogen removal by heterotrophic nitrifying-denitrifying bacteria were conducted at C/N ratio of 10 [ 12 , 42 ]. It was suggested that insufficient carbon supply impairs both microbial growth and electron donors for denitrification [ 57 , 58 ]. Hence, fewer requirements for C/N ratio in the reservoir source water experiment would be favorable for the nitrogen removal of oligotrophic source water.…”

Section: Discussionmentioning

confidence: 99%

Nitrogen Removal Characteristics of a Newly Isolated Indigenous Aerobic Denitrifier from Oligotrophic Drinking Water Reservoir, Zoogloea sp. N299

Huang

Zhou

Zhang

et al. 2015

IJMS

View full text Add to dashboard Cite

Nitrogen is considered to be one of the most widespread pollutants leading to eutrophication of freshwater ecosystems, especially in drinking water reservoirs. In this study, an oligotrophic aerobic denitrifier was isolated from drinking water reservoir sediment. Nitrogen removal performance was explored. The strain was identified by 16S rRNA gene sequence analysis as Zoogloea sp. N299. This species exhibits a periplasmic nitrate reductase gene (napA). Its specific growth rate was 0.22 h−1. Obvious denitrification and perfect nitrogen removal performances occurred when cultured in nitrate and nitrite mediums, at rates of 75.53% ± 1.69% and 58.65% ± 0.61%, respectively. The ammonia removal rate reached 44.12% ± 1.61% in ammonia medium. Zoogloea sp. N299 was inoculated into sterilized and unsterilized reservoir source waters with a dissolved oxygen level of 5–9 mg/L, pH 8–9, and C/N 1.14:1. The total nitrogen removal rate reached 46.41% ± 3.17% (sterilized) and 44.88% ± 4.31% (unsterilized). The cell optical density suggested the strain could survive in oligotrophic drinking water reservoir water conditions and perform nitrogen removal. Sodium acetate was the most favorable carbon source for nitrogen removal by strain N299 (p < 0.05). High C/N was beneficial for nitrate reduction (p < 0.05). The nitrate removal efficiencies showed no significant differences among the tested inoculums dosage (p > 0.05). Furthermore, strain N299 could efficiently remove nitrate at neutral and slightly alkaline and low temperature conditions. These results, therefore, demonstrate that Zoogloea sp. N299 has high removal characteristics, and can be used as a nitrogen removal microbial inoculum with simultaneous aerobic nitrification and denitrification in a micro-polluted reservoir water ecosystem.

show abstract

Section: Discussionmentioning

confidence: 99%

Nitrogen Removal Characteristics of a Newly Isolated Indigenous Aerobic Denitrifier from Oligotrophic Drinking Water Reservoir, Zoogloea sp. N299

Huang

Zhou

Zhang

et al. 2015

IJMS

View full text Add to dashboard Cite

show abstract

“…is a heterotrophic nitrification-aerobic denitrifying bacteria isolated from MBR system. It can not only oxidize ammonia nitrogen to nitrite, but also reduce nitrite to nitrogen [25]. Acinetobacter calcoaceticus can convert ammonia to nitrate, but could not reduce nitrate to nitrite or nitrogen gas under aerobic conditions [26].…”

Section: Resultsmentioning

confidence: 99%

Effects of Temperature on the Characteristics of Nitrogen Removal and Microbial Community in Post Solid-Phase Denitrification Biofilter Process

Zhang

Chen

Luo

et al. 2019

IJERPH

View full text Add to dashboard Cite

In order to solve the problems of high energy consumption, complex process and low nitrogen removal efficiency in the currently available low carbon source wastewater treatment processes, a novel coagulation sedimentation/post-solid-phase denitrification biofilter process (CS-BAF-SPDB) was proposed. The effect of temperature on the nitrogen removal performance of BAF-SPDB was intensively studied, and the mechanism of the effect of temperature on nitrogen removal performance was analyzed from the perspective of microbial community structure by using the polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE). The results showed that, to realize favorable nitrifying and denitrifying performance simultaneously in the BAF-SPDB unit, the operation temperature should be set above 18 °C. In addition, the influence of the macro operation parameters on the performance of the BAF and SPDB has a direct relationship with the dynamic changes of the micro microbial community. The influence of temperature on nitrification performance in BAF was mainly embodied in the change of composition, amount and activity of ammonia oxidizing bacteria Candidatus Nitrospira defluvii and nitrite oxidizing bacteria Nitrosomonas sp. Nm47, while that on denitrification performance in SPDB is mainly embodied in the change of composition and amount of solid carbon substrate degrading denitrifying bacteria Pseudomonas sp., Myxobacterium AT3-03 and heterotrophic denitrifying bacteria Dechloromonas agitate, Thauera aminoaromatica, Comamonas granuli and Rubrivivax gelatinosus.

show abstract

“…The amoA gene encodes the subunit A of the enzyme ammonia monoxygenase (catalyzes the oxidation of ammonia in hydroxylamine) that together with amoC and amoB are part of the AMO operon present in all the chemilitotrophic AOBs studied (Nitrosomonas europaea, Nitrosaspira multiformis, Nitrosococcus oceanus and Nitosospira sp). In the genus Bacillus, the amoA gene has been poorly studied, finding only a report of the presence of the amoA gene in the strain Bacillus sp LY where they also determined that the amoB gene was absent unlike what was found in AOB where the amoA and amoB gene are co-transcribed which suggests that amoB would not be part of the same transcriptional unit and, therefore, is not a member of the master operon in Bacillus sp LY 17 . In the present study, the amoA gene was absent in the four strains that have the ability to remove ammonia, which raises doubts, since it could be due to the primers used in both studies, which differ in the sequences.…”

Section: Identification Of Genes Involved In Nitrification and Denitrmentioning

confidence: 99%

Assessment of Heterotrophic Nitrification Capacity in Bacillus spp. and its Potential Application in the Removal of Nitrogen from Aquaculture Water

Mendoza¹,

Mujica²,

Cunayque³

et al. 2019

J Pure Appl Microbiol

View full text Add to dashboard Cite

The accumulation of nitrogen (-N) is a serious problem in aquaculture as it could lead to mass mortality events of the cultivated species. Chemilitotrophic nitrification is the most recognized in nitrogen removal underestimating the role of heterotrophic nitrifiers. In the present study, the heterotrophic nitrification capacity of 8 bacterial strains isolated from mangrove soil, periphyton and biofilters was evaluated. The strains were grown in heterotrophic nitrification base medium (HNM medium) with three different nitrogen sources, ammonium, nitrite or nitrate at a final concentration of 8 mg L -1 , 5 mg L -1 and 80 mg L -1 respectively. The concentration of nitrogen (-N) and OD (600 nm) were determined periodically. Only in 4 strains belonging to the Bacillus genus was the capacity for heterotrophic nitrification and aerobic denitrification observed. Among these strains, SM4 strain presented a good removal profile of ammonium, nitrite and nitrate, achieving an average nitrification efficiency of 98. 33 ± 2.89%, 83.67 ± 7.51% and 98.00 ± 0.01% respectively, and a nitrification rate (mg L -1 h -1 ) of 1.71 ± 0.70, 0.13 ± 0.07 and 0.21 ± 0.06 respectively. The nxrB, nirS, nirK genes in the selected strains were identified by PCR. Additionally, several proteins (enzymes) involved in the nitrogen cycle were identified by proteomic analysis, reporting for the first time the presence of the enzymes ammonia monoxygenase (AMO) and nitrite oxide reductase (NXR) in the genus Bacillus. These findings suggest that the strains studied would have a potential use in the biological removal of nitrogen in aquaculture systems.

show abstract

Physiological and molecular biological characteristics of heterotrophic ammonia oxidation by Bacillus sp. LY

Cited by 27 publications

References 33 publications

Nitrogen Removal Characteristics of a Newly Isolated Indigenous Aerobic Denitrifier from Oligotrophic Drinking Water Reservoir, Zoogloea sp. N299

Nitrogen Removal Characteristics of a Newly Isolated Indigenous Aerobic Denitrifier from Oligotrophic Drinking Water Reservoir, Zoogloea sp. N299

Effects of Temperature on the Characteristics of Nitrogen Removal and Microbial Community in Post Solid-Phase Denitrification Biofilter Process

Assessment of Heterotrophic Nitrification Capacity in Bacillus spp. and its Potential Application in the Removal of Nitrogen from Aquaculture Water

Contact Info

Product

Resources

About