Physiological and Proteomic Responses of Cassava to Short-Term Extreme Cool and Hot Temperature

Abstract

Temperature is one of the most critical factors affecting cassava metabolism and growth. This research was conducted to investigate the effects of short-term exposure to extreme cool (15 °C) and hot (45 °C) temperature on photosynthesis, biochemical and proteomics changes in potted plants of two cassava cultivars, namely Rayong 9 and Kasetsart 50. One-month-old plants were exposed to 15, 30, and 45 °C for 60 min in a temperature chamber under light intensity of 700 μmol m−2 s−1. Compared to the optimum temperature (30 °C), exposure to 15 °C resulted in 28% reduction in stomatal conductance (gs) and 62% reduction in net photosynthesis rate (Pn). In contrast, gs under 45 °C increased 2.61 folds, while Pn was reduced by 50%. The lower Pn but higher electron transport rate (ETR) of the cold-stressed plants indicated that a greater proportion of electrons was transported via alternative pathways to protect chloroplast from being damaged by reactive oxygen species (ROS). Moreover, malondialdehyde (MDA) contents, a marker related to the amount of ROS, were significantly higher at low temperature. Proteomics analysis revealed some interesting differentially expressed proteins (DEPs) including annexin, a multi-functional protein functioning in early events of heat stress signaling. In response to low-temperature stress, AP2/ERF domain-containing protein (a cold-related transcription factor) and glutaredoxin domain-containing protein (a component of redox signaling network under cold stress) were detected. Taken together, both cultivars were more sensitive to low than high temperature. Moreover, Rayong 9 displayed higher Pn under both temperature stresses, and was more efficient in controlling ROS under cold stress than Kasetsart 50.

Variations in Cold Resistance and Contents of Bioactive Compounds among Dendrobium officinale Kimura et Migo Strains

“…MDA is a lipid peroxidation product, and its content is used to assess the degree of lipid peroxidation. A low content indicates lower oxidative damage to the plant cell and organelle membranes and enhanced tolerance to low temperature [39]. The MDA content was diminished in all four strains, with Fujian showing the maximum decrease, followed by Xianju, indicating a certain degree of cold resistance (Figure 3B).…”

Estimasi Jejak Karbon pada Budidaya Ubi kayu dari Berbagai Dosis Pupuk NPK dan Karbon Organik Tanah

“…Pemanasan global secara umum memicu perubahan iklim yang berkontribusi negatif pada produksi tanaman (Dulbari et al, 2021;Purwono et al, 2021). Pada tanaman ubi kayu, peningkatan suhu berdampak negatif pada pertumbuhan dan hasil umbi (Santanoo et al, 2022).…”

A data‐driven genome annotation approach for cassava

“…Cassava cultivation is limited by susceptibility to cold temperatures, likely restricting cultivation in distribution the Northern Hemisphere (An et al, 2012;Santanoo et al, 2022 pathways for cold resilience in many plant species begin with the transcriptional induction of homologs of Arabidopsis C-REPEAT BINDING FACTORS (CBF1-3) genes (Barrero-Gil & Salinas, 2018;Thomashow, 2003). In cassava, homology searches support the existence of at least some aspects of this cold-resilience pathway (Fan et al, 2016).…”

“…Cassava cultivation is limited by susceptibility to cold temperatures, likely restricting cultivation in distribution the Northern Hemisphere (An et al., 2012; Santanoo et al., 2022). In the context of rising global temperatures, cultivation of cold‐resilient cassava varieties may become an attractive option for future farming in the global north.…”