Physiological and transcription analyses reveal the regulatory mechanism of melatonin in inducing drought resistance in loquat (Eriobotrya japonica Lindl.) seedlings

Wang, Dan; Chen, Qiyang; Chen, Weiwei; Guo, Qigao; Xia, Yan; Wang, Shuming; Jing, Danlong; Liang, Guolu

doi:10.1016/j.envexpbot.2020.104291

Cited by 62 publications

(40 citation statements)

References 75 publications

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“…In addition, the application of melatonin increased the root sugar content of sugar beets exposed to salt stress ( Figure 1 F). A potential reason for this positive effect may be that the application of melatonin is beneficial for the Calvin cycle and the activities of enzymes involved in sucrose synthesis [ 47 ], thus increasing the sugar content in the cytoplasm of sugar beets under salt stress conditions.…”

Section: Discussionmentioning

confidence: 99%

Beneficial Effects of Exogenous Melatonin on Overcoming Salt Stress in Sugar Beets (Beta vulgaris L.)

Zhang

Liu

Wang

et al. 2021

Plants

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Melatonin has been regarded as a promising substance that enhances the abiotic stress tolerance of plants. However, few studies have devoted attention to the role of melatonin in improving salt tolerance in sugar beets. Here, the effects of different application methods (foliar application (100 μM), root application (100 μM), and combined foliar and root application) of melatonin on the morphological and physiological traits of sugar beets exposed to salt stress were investigated. The results showed that melatonin improved the growth of sugar beet seedlings, root yield and sugar content, synthesis of chlorophyll, photosystem II (PS II) activity, and gas exchange parameters under salt stress conditions. Moreover, melatonin enhanced the capacity of osmotic adjustment by increasing the accumulation of osmolytes (betaine, proline, and soluble sugar). At the same time, melatonin increased the H+-pump activities in the roots, thus promoting Na+ efflux and K+ influx, which maintained K+/Na+ homeostasis and mitigated Na+ toxicity. In addition, melatonin strengthened the antioxidant defense system by enhancing the activities of antioxidant enzymes, modulating the ASA-GSH cycle, and mediating the phenylalanine pathway, which removed superoxide anions (O2•−) and hydrogen peroxide (H2O2) and maintained cell membrane integrity. These positive effects were more pronounced when melatonin was applied by combined foliar and root application. To summarize, this study clarifies the potential roles of melatonin in mitigating salt stress in sugar beets by improving photosynthesis, water status, ion homeostasis, and the antioxidant defense system.

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Section: Discussionmentioning

confidence: 99%

Beneficial Effects of Exogenous Melatonin on Overcoming Salt Stress in Sugar Beets (Beta vulgaris L.)

Zhang

Liu

Wang

et al. 2021

Plants

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“…Drought directly affects the growth and productivity of plants. Under drought stress, the total chlorophyll content and photosynthetic rates of loquat leaves were decreased, resulting in a decline in starch content [ 27 ]. Severe drought reduced all growth parameters and particularly leaf growth [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

“…The excessive ROS production triggered by drought caused lipid peroxidation, protein oxidation, nucleic acid damage, and even cell death in plants [ 36 ]. When loquat seedlings were subjected to drought stress, the MDA content of loquat leaves exhibited an increase followed by a gradual drop [ 27 ]. The MDA concentration is a parameter of membrane lipid peroxidation, which reflects the extent of stress [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

“…For instance, WRKY18 and WRKY60 conferred hypersensitivity to ABA stress in inhibiting seed germination and root growth, while WRKY40 antagonized WRKY18 and WRKY60 in response to sensitivity to ABA and the abiotic stress [ 39 ]. The ABA content of loquat leaves gradually accumulated and ABA biosynthetic genes were significantly regulated under drought stress [ 27 ]. Moreover, ABA-mediated stomatal closure provided a vital strategy in plant stress responses to prevent water loss [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

“…However, the growth, development, and yield of loquat are often affected by multiple abiotic stresses, especially drought and cold stresses [ 26 ]. Our previous research showed that the WRKYs family was involved in the drought stress in loquat seedlings [ 27 ]. However, the function and molecular mechanism of WRKY TF family members in loquat remains elusive.…”

Section: Introductionmentioning

confidence: 99%

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A WRKY Transcription Factor, EjWRKY17, from Eriobotrya japonica Enhances Drought Tolerance in Transgenic Arabidopsis

Wang

Chen

et al. 2021

IJMS

Self Cite

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The WRKY gene family, which is one of the largest transcription factor (TF) families, plays an important role in numerous aspects of plant growth and development, especially in various stress responses. However, the functional roles of the WRKY gene family in loquat are relatively unknown. In this study, a novel WRKY gene, EjWRKY17, was characterized from Eriobotrya japonica, which was significantly upregulated in leaves by melatonin treatment during drought stress. The EjWRKY17 protein, belonging to group II of the WRKY family, was localized in the nucleus. The results indicated that overexpression of EjWRKY17 increased cotyledon greening and root elongation in transgenic Arabidopsis lines under abscisic acid (ABA) treatment. Meanwhile, overexpression of EjWRKY17 led to enhanced drought tolerance in transgenic lines, which was supported by the lower water loss, limited electrolyte leakage, and lower levels of reactive oxygen species (ROS) and malondialdehyde (MDA). Further investigations showed that overexpression of EjWRKY17 promoted ABA-mediated stomatal closure and remarkably up-regulated ABA biosynthesis and stress-related gene expression in transgenic lines under drought stress. Overall, our findings reveal that EjWRKY17 possibly acts as a positive regulator in ABA-regulated drought tolerance.

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Molecular Physiology of Melatonin Induced Temperature Stress Tolerance in Plants

Sharma,

Pandey

2023

Plant in Challenging Environments

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Physiological and transcription analyses reveal the regulatory mechanism of melatonin in inducing drought resistance in loquat (Eriobotrya japonica Lindl.) seedlings

Cited by 62 publications

References 75 publications

Beneficial Effects of Exogenous Melatonin on Overcoming Salt Stress in Sugar Beets (Beta vulgaris L.)

Beneficial Effects of Exogenous Melatonin on Overcoming Salt Stress in Sugar Beets (Beta vulgaris L.)

A WRKY Transcription Factor, EjWRKY17, from Eriobotrya japonica Enhances Drought Tolerance in Transgenic Arabidopsis

Molecular Physiology of Melatonin Induced Temperature Stress Tolerance in Plants

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