Physiological and transcriptional analysis of Chinese soft-shelled turtle (Pelodiscus sinensis) in response to acute nitrite stress

Feng, Rui; Zhang, Zhao; Guan, Yueqiang

doi:10.1016/j.aquatox.2021.105899

Cited by 15 publications

(10 citation statements)

References 38 publications

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“…Alligator snapping turtle North America Aquatic [61] List of Testudines' immune response reviewed, regarding species name, habitat, and ecosystem. * Sea turtles lack significant studies on the immune response.…”

Section: Chelydra Serpentinamentioning

confidence: 99%

“…The complement cascade involves a series of proteins in plasma that, when activated, can lead to the formation of the membrane attack complex (MAC) that causes lysis when inserted into microbial cell walls. In addition, complement activation initiates the recruitment of leukocytes to the site of infection, phagocytosis, and expression of pro-inflammatory cytokines (Figure 3) [61,152].…”

Section: Innate Immune Effectorsmentioning

confidence: 99%

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Immunity in Sea Turtles: Review of a Host-Pathogen Arms Race Millions of Years in the Running

Nash

Ryan

2023

Animals

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The immune system of sea turtles is not completely understood. Sea turtles (as reptiles) bridge a unique evolutionary gap, being ectothermic vertebrates like fish and amphibians and amniotes like birds and mammals. Turtles are ectotherms; thus, their immune system is influenced by environmental conditions like temperature and season. We aim to review the turtle immune system and note what studies have investigated sea turtles and the effect of the environment on the immune response. Turtles rely heavily on the nonspecific innate response rather than the specific adaptive response. Turtles’ innate immune effectors include antimicrobial peptides, complement, and nonspecific leukocytes. The antiviral defense is understudied in terms of the diversity of pathogen receptors and interferon function. Turtles also mount adaptive responses to pathogens. Lymphoid structures responsible for lymphocyte activation and maturation are either missing in reptiles or function is affected by season. Turtles are a marker of health for their marine environment, and their immune system is commonly dysregulated because of disease or contaminants. Fibropapillomatosis (FP) is a tumorous disease that afflicts sea turtles and is thought to be caused by a virus and an environmental factor. We aim, by exploring the current understanding of the immune system in turtles, to aid the investigation of environmental factors that contribute to the pathogenesis of this disease and provide options for immunotherapy.

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Section: Chelydra Serpentinamentioning

confidence: 99%

Section: Innate Immune Effectorsmentioning

confidence: 99%

Immunity in Sea Turtles: Review of a Host-Pathogen Arms Race Millions of Years in the Running

Nash

Ryan

2023

Animals

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“…The Chinese soft-shelled turtle ( Pelodiscus sinensis ) belongs to the genus Pelodiscus of the family Trionychidae, and it is recognized as an important economic species with nutritional value and medicinal value [ 1 , 2 , 3 ]. P. sinensis is widely distributed in East Asia, including China, Korea, Japan, and Vietnam [ 4 , 5 ]. According to the geographical distribution, P. sinensis can be divided into different strains, including the Japanese strain, Yellow River strain, Huaihe River strain, Poyang Lake strain, Taihu Lake strain, Southwest strain, Taiwan strain, etc.…”

Section: Introductionmentioning

confidence: 99%

Complete Mitochondrial Genomes of Four Pelodiscus sinensis Strains and Comparison with Other Trionychidae Species

Chen

Jiao

Yuan

et al. 2023

Biology

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The Chinese soft-shelled turtle (Pelodiscus sinensis) is an important aquaculture reptile with rich nutritional and medicinal values. In recent decades, the wild resources of P. sinensis have been depleting due to natural and artificial factors. Herein, we report the complete mitochondrial genome of four P. sinensis strains, including the Japanese (RB) strain, Qingxi Huabie (HB) strain, Jiangxi (JB) strain, and Qingxi Wubie (WB) strain. The nucleotide composition within the complete mitogenomes was biased towards A + T with a variable frequency ranging from 59.28% (cox3) to 70.31% (atp8). The mitogenomes of all four strains contained 13 protein-coding genes (PCGs), 22 tRNAs, 2 rRNAs, 1 control region, and a replication origin region of the L-strand replication (OL), which was consistent with most vertebrates. Additionally, the atp8, nad4l, nad6, and nad3 genes possessed high genetic variation and can be used as potential markers for the identification of these P. sinensis strains. Additionally, all PCGs genes were evolving primarily under purifying selection. Through comparative analysis, it was revealed that most of the tRNAs were structurally different in the TψC stem, DHU stem, and acceptor stem. The length of the tandem repeats in the control region was variable in the four P. sinensis strains, ranging from 2 bp to 50 bp. Phylogenetic analysis indicated that all P. sinensis strains clustered into one branch and were closely related to other Trionychinae species. Overall, this study provides mitochondrial genome information for different P. sinensis strains to support further species identification and germplasm resource conservation.

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“…Several factors have been reported to affect the output of P. sinensis, including stocking density, management factors, and water quality (Rangel-Mendoza et al 2014). Environmental factors, especially those of aquaculture water, have an in uence on the health of aquatic animals (Feng et al 2021). Nitrite accumulation causes serious problems in the growth, survival, and reproduction of aquatic animals (Kocour Kroupová et al 2018;Feng et al 2021).…”

Section: Introductionmentioning

confidence: 99%

“…Environmental factors, especially those of aquaculture water, have an in uence on the health of aquatic animals (Feng et al 2021). Nitrite accumulation causes serious problems in the growth, survival, and reproduction of aquatic animals (Kocour Kroupová et al 2018;Feng et al 2021). In addition, pollutants such as dissolved organic matter are produced by excess food and feces of turtles in greenhouse tanks (Wu et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of bacterial diversity of pond water with different output of Chinese soft-shelled turtle Pelodiscus sinensis in dark greenhouse mode

Wang,

Chang,

Yan

et al. 2023

Preprint

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Dark greenhouse culture is one of the main culture methods of the Chinese soft-shelled turtle, Pelodiscus sinensis. The present study found that the outputs of P. sinensis differed among five ponds with the same initial stocking density and management measures, with the output of pond B with maximum yield being 1.69 times higher than that of pond A with minimum yield. Therefore, the physicochemical factors and bacterial communities of the two ponds were monitored, analyzed and compared. The contents of total nitrogen, nitrite nitrogen, and dissolved oxygen in pond B were extremely significantly higher than those in pond A at the late stage of cultivation. Correspondingly, the microbial diversity and richness were also higher in pond B. Additionally, the higher relative abundances of Gemmatimonadetes, Firmicutes, Nitrosomonas, Saccharimonadales andAzospira in pond B conferred it higher nitrification and denitrification ability than pond A in the middle and late stage of cultivation. Pearson’s correlation coefficient showed that the Saccharimonadales andAzospira were correlated with total nitrogen, ammonia nitrogen, nitrite nitrogen, and nitrate nitrogen. Our results provide a reference for the modulation of the water environment in the dark greenhouse mode that used for turtle cultivation.

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Physiological and transcriptional analysis of Chinese soft-shelled turtle (Pelodiscus sinensis) in response to acute nitrite stress

Cited by 15 publications

References 38 publications

Immunity in Sea Turtles: Review of a Host-Pathogen Arms Race Millions of Years in the Running

Immunity in Sea Turtles: Review of a Host-Pathogen Arms Race Millions of Years in the Running

Complete Mitochondrial Genomes of Four Pelodiscus sinensis Strains and Comparison with Other Trionychidae Species

Comparative analysis of bacterial diversity of pond water with different output of Chinese soft-shelled turtle Pelodiscus sinensis in dark greenhouse mode

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