Physiological and transcriptome profiling revealed defense networks during Cladosporium fulvum and tomato interaction at the early stage

Abstract

Tomato leaf mold caused by Cladosporium fulvum (C. fulvum) is a serious fungal disease which results in huge yield losses in tomato cultivation worldwide. In our study, we discovered that ROS (reactive oxygen species) burst was triggered by C. fulvum treatment in tomato leaves. RNA-sequencing was used to identify differentially expressed genes (DEGs) induced by C. fulvum inoculation at the early stage of invasion in susceptible tomato plants. Gene ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases were used to annotate functions of DEGs in tomato plants. Based on our comparative analysis, DEGs related to plant-pathogen interaction pathway, plant hormone signal transduction pathway and the plant phenylpropanoid pathway were further analyzed. Our results discovered that a number of core defense genes against fungal invasion were induced and plant hormone signal transduction pathways were impacted by C. fulvum inoculation. Further, our results showed that SA (salicylic acid) and ABA (abscisic acid) contents were accumulated while JA (jasmonic acid) content decreased after C. fulvum inoculation in comparison with control, and quantitative real-time PCR to detect the relative expression of genes involved in SA, ABA and JA signaling pathway further confirmed our results. Together, results will contribute to understanding the mechanisms of C. fulvum and tomato interaction in future.

A new versatile peroxidase with extremophilic traits over-produced in MicroTom cell cultures

“…1 A). The Peptide Mass Fingerprint analysis of the tryptic peptides gave a significant match with peroxidase from Solanum lycopersicum and it was precisely recognized as the LePrx21 gene product, a suberization-associated anionic peroxidase 2-like (NCBI Reference Sequence: XP_004234138.1; UniProtKB accession A0A3Q7FFR5), which consisted of 334 amino acids and was implicated in the defence response to environmental stresses such as wounding, Mn excess 10 , pathogen attack or oxidative stress 11 , 12 . The total coverage of the protein sequence was 64% (Fig.…”

Amorphophallus muelleri activates ferulic acid and phenylpropane biosynthesis pathways to defend against Fusarium solani infection

“…With advancements in next-generation sequencing, RNA-Seq has become an important means for researchers to study the mechanism of plant disease resistance. Through physiological index determination and transcriptome sequencing, Peng et al (2022) showed that the expression of multiple core defense genes involved in fungal disease resistance and hormone signaling pathways is induced in tomato (Solanum lycopersicum) upon Cladosporium fulvum infection. Wei et al (2022) sequenced the transcriptome of Amorphophallus spp.…”