Physiological and transcriptomic analysis revealed the involvement of crucial factors in heat stress response of Rhododendron hainanense

Zhao, Ying; Yu, Wengang; Hu, Xiangyu; Shi, Youhai; Yu, Liu; Zhong, Yunfang; Wang, Peng; Deng, Shuya; Niu, Jun; Yu, Xudong

doi:10.1016/j.gene.2018.03.082

Cited by 29 publications

(21 citation statements)

References 46 publications

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“…Such differences likely contributed to functional divergences. NAC92 and NAC59 are involved in senescence, salt stress responses, and lateral root development ( Balazadeh et al, 2010 ), whereas NAC29 is involved in heat stress responses ( Zhao et al, 2018 ).…”

Section: Resultsmentioning

confidence: 99%

Evolution of Protein Ductility in Duplicated Genes of Plants

et al. 2018

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Previous work has shown that ductile/intrinsically disordered proteins (IDPs) and residues (IDRs) are found in all unicellular and multicellular organisms, wherein they are essential for basic cellular functions and complement the function of rigid proteins. In addition, computational studies of diverse phylogenetic lineages have revealed: (1) that protein ductility increases in concert with organismic complexity, and (2) that distributions of IDPs and IDRs along the chromosomes of plant species are non-random and correlate with variations in the rates of the genetic recombination and chromosomal rearrangement. Here, we show that approximately 50% of aligned residues in paralogs across a spectrum of algae, bryophytes, monocots, and eudicots are IDRs and that a high proportion (ca. 60%) are in disordered segments greater than 30 residues. When three types of IDRs are distinguished (i.e., identical, similar and variable IDRs) we find that species with large numbers of chromosome and endoduplicated genes exhibit paralogous sequences with a higher frequency of identical IDRs, whereas species with small chromosomes numbers exhibit paralogous sequences with a higher frequency of similar and variable IDRs. These results are interpreted to indicate that genome duplication events influence the distribution of IDRs along protein sequences and likely favor the presence of identical IDRs (compared to similar IDRs or variable IDRs). We discuss the evolutionary implications of gene duplication events in the context of ductile/disordered residues and segments, their conservation, and their effects on functionality.

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Section: Resultsmentioning

confidence: 99%

Evolution of Protein Ductility in Duplicated Genes of Plants

et al. 2018

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“…Moreover, in response to heat stress, the proline content was higher for each hybrid as compared with their respective controls (25°C) at all durations. Previous studies also demonstrated that as temperature increases proline accumulation also increased in response to heat shock on R. hainanense (Zhao et al, 2018). Proline accumulation in response to heat stress is controversial among plant scientists.…”

Section: Discussionmentioning

confidence: 98%

Physiological response of three micropropagated plantlets of Rhododendron hybrids for heat stress

Oba¹,

Hu²,

Zhang³

et al. 2021

Europ.J.Hortic.Sci

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“…In the study of rose (Rosa chinensis 'Slater's crimson China') heat resistance, it was found that both NAC27 and NAC72 were up-regulated [31]. NAC27 and NAC72 were also involved in ABA signal transduction under heat stress of Rhododendron hainanensis [32]. Overexpression of NAC72 in Arabidopsis thaliana can increase the drought resistance of plants, and it also proved that it mediates the regulation of ABA-responsive genes [33].…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome Analysis Reveals Molecular Mechanism and Regulatory Network of Rosa Chinensis ‘Old Blush’ Leaves in Response to Drought Stress

Jia¹,

Feng²,

Bu³

et al. 2020

Preprint

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Background: Rose is one of the most important ornamental crops, but drought limits its yield and quality seriously. Although serval abiotic stress related genes have been identified, the molecular mechanism during rose’s responding to drought stress is still limited. In this study, the transcriptomes of leaves of two-year-old cutting seedlings of Rosa chinensis ’Old Blush’ from three continuous droughted stages (30, 60, 90 days after full watering) and rewatering were analyzed using RNA sequencing. Meanwhile, weighted gene co-expression network analysis (WGCNA) was used to construct a co-expression network associated with the physiological traits of drought response to discover the hub transcription factors involved in drought response.Results: More than 45 million high-quality clean reads were generated from samples and used for comparison with the rose reference genome. A total of 46433 differentially expressed genes were identified. Gene ontology (GO) term enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated that drought stress caused significant changes in signal transduction, plant hormones, primary and secondary metabolism, and a certain degree of recovery after rewatering. Gene co-expression analysis identified 18 modules, in which four modules have highly degree of correlation with physiological traits. In addition, twenty-two transcription factors including members of NACs, WRKYs, MYBs, ERFs, ARFs and bHLHs with high connectivity in these four modules were screened. Conclusions: The results of this study provided the first transcriptome sequencing report of the drought stress in R.chinensis, and also provided candidate transcription factors in response to drought stress, providing clues for improving the drought tolerance of the rose through molecular breeding in the future.

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Physiological and transcriptomic analysis revealed the involvement of crucial factors in heat stress response of Rhododendron hainanense

Cited by 29 publications

References 46 publications

Evolution of Protein Ductility in Duplicated Genes of Plants

Evolution of Protein Ductility in Duplicated Genes of Plants

Physiological response of three micropropagated plantlets of Rhododendron hybrids for heat stress

Comparative Transcriptome Analysis Reveals Molecular Mechanism and Regulatory Network of Rosa Chinensis ‘Old Blush’ Leaves in Response to Drought Stress

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