Physiological characteristics and related gene expression of after‐ripening on seed dormancy release in rice

Du, Wenli; Jiaan, Cheng; Cheng, Yuxiang; Wang, L.; He, Yu; Wang, Z.; Zhang, H.

doi:10.1111/plb.12371

Cited by 27 publications

(14 citation statements)

References 53 publications

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“…The segregation of PHS to normal phenotype in two mutant plants fits the 1 : 3 ratios with probabilities of 0.38 (χ 2 = 0.77 <

χ_{0.05, 1}^{2}

= 3.84) and 0.10 (χ 2 = 2.69 <

χ_{0.05, 1}^{2}

= 3.84) by χ 2 ‐square test, respectively. Dormancy release was ordinarily required for germination of fresh harvest seeds in rice (Du et al ., ; Shu et al ., ). However, seed germination rates (36 h) of fresh harvest from two mutant progeny plants, which did not go through dormancy release, were 23.3% and 26.0%, respectively, and were significant higher than that of the wild‐type (0.7%) (Fig.…”

Section: Resultsmentioning

confidence: 99%

Identification and characterization of the rice pre‐harvest sprouting mutants involved in molybdenum cofactor biosynthesis

Liu

Wang

et al. 2018

New Phytologist

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In cereal crops, ABA deficiency during seed maturation phase causes pre-harvest sprouting (PHS), and molybdenum cofactor (MoCo) is required for ABA biosynthesis.Here, two rice PHS mutants F254 and F5-1 were characterized. In addition to the PHS, these mutants showed pleiotropic phenotypes such as twisting and slender leaves, and then died when the seedling developed to four or five leaves. Map-based cloning showed that OsCNX6 and OsCNX1 encoding homologs of MoaE and MoeA were responsible for F254 and F5-1 mutants, respectively. Genetic complementation indicated that OsCNX6 not only rescued the PHS and seedling lethal phenotype of the cnx6 mutant, but also recovered the MoCo-dependent enzyme activities such as xanthine dehydrogenase (XDH), aldehyde oxidase (AO), nitrate reductase (NR) and sulfite oxidase (SO).Expression pattern showed that OsCNX6 was richly expressed in seed during embryo maturation by quantitative reverse transcriptase PCR and RNA in situ hybridization. Furthermore, the OsCNX6 overexpression plants can significantly enhance the MoCo-dependent enzyme activities, and improved the osmotic and salt stress tolerance without unfavorable phenotypes.Collectively, these data indicated that OsCNX6 participated in MoCo biosynthesis, and is essential for rice development, especially for seed dormancy and germination, and OsCNX6 could be an effective target for improving abiotic stress tolerance in rice.

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“…The segregation of PHS to normal phenotype in two mutant plants fits the 1 : 3 ratios with probabilities of 0.38 (χ 2 = 0.77 <

χ_{0.05, 1}^{2}

= 3.84) and 0.10 (χ 2 = 2.69 <

χ_{0.05, 1}^{2}

Section: Resultsmentioning

confidence: 99%

Identification and characterization of the rice pre‐harvest sprouting mutants involved in molybdenum cofactor biosynthesis

Liu

Wang

et al. 2018

New Phytologist

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“…japonica ) was used from the Taihu Lake valley in Jiangsu Province of China (Du et al, 2015; Cheng et al, 2017). Seeds were provided by the Laboratory of Seed Science and Technology in Nanjing Agricultural University (Nanjing, Jiangsu Province, China).…”

Section: Methodsmentioning

confidence: 99%

“…The weight of imbibed seeds was recorded each 12 h to calculate the moisture content of seeds. Germination ability was observed every day to calculate germination percentage (GP) and seedling percentage (SP) (Du et al, 2015). Seeds were considered as germination when the radicle protruded (2 mm) through seed coat.…”

Section: Methodsmentioning

confidence: 99%

Proteomic Analysis Reveals Proteins Involved in Seed Imbibition under Salt Stress in Rice

Chen

et al. 2017

Front. Plant Sci.

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Enhancement of salinity tolerance during seed germination is very important for direct seeding in rice. In this study, the salt-tolerant japonica landrace Jiucaiqing was used to determine the regulators that are involved in seed imbibition under salt stress. Briefly, the comparative proteomic analysis was conducted between dry (0 h) and imbibed (24 h) seeds with 150 mM NaCl. Under salt stress, the uptake of water increased rapidly before 24 h imbibition (Phase I), followed by a plateau of seed imbibition from 24 to 96 h imbibition (Phase II). We identified 14 proteins involved in seed imbibition, in which the majority of these proteins were involved in energy supply and storage protein. The early imbibition process was mediated by protein catabolism; the most of proteins were down-regulated after 24 h imbibition. Eleven genes in salt stress treated seeds were expressed early during the seed imbibition in comparison to control seeds. By comparison, 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (BPM), glutelin (GLU2.2 and GLU2.3), glucose-1-phosphate adenylyltransferase large subunit (GAS8), and cupin domain containing protein (CDP3.1 and CDP3.2) were near the regions of quantitative trait loci (QTLs) for seed dormancy, seed reserve utilization, and seed germination in Jiucaiqing. In particular, CDP3.1 was co-located in the region of qIR-3 for imbibition rate, and qGP-3 for germination percentage. The role of CDP3.1 was verified in enhancing seed germination under salt stress using T-DNA mutant. The identified proteins might be applicable for the improvement of seed germination under salt stress in rice.

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“…Consistently, RNAi-mediated knock-down of HvABA8 ′ OH1 leads to enhanced seed ABA level and dormancy ( Gubler et al, 2008 ). Studies in rice also indicated that the reduction of ABA level during imbibition of non-dormant seeds of rice is related mainly with increased expression of OsABA8ox (rice homologs of CYP707A ) genes ( Zhu et al, 2009 ; Du et al, 2015 ). These results suggest the significance of ABA catabolism in the regulation of ABA level and seed dormancy maintenance and release in the seeds of cereals such as rice and barley.…”

Section: Aba Regulates the Induction And Maintenance Of Seed Dormancymentioning

confidence: 99%

“…Consistently, comparative genomic analysis among barley, wheat, and rice identified HvGA20ox as a candidate gene regulating a seed dormancy QTL in barley ( Li et al, 2004 ). Likewise, the expression of the GA biosynthetic genes ( GA20ox and GA3ox2 ) is induced while that of GA inactivating genes ( GA2ox ) is repressed during imbibition of non-dormant rice and sorghum seeds, and this has been shown to be associated with increased level of bioactive GA ( Kaneko et al, 2002 ; Rodríguez et al, 2012 ; Du et al, 2015 ). Recent genetic studies in rice have identified OsGA20ox2 and OsGA2ox3 as candidate genes for controlling seed germination ( Ye et al, 2015 ; Magwa et al, 2016 ), and loss of function mutation in OsGA20ox2 leads to reduced seed GA level and enhanced dormancy ( Ye et al, 2015 ).…”

Section: Gibberellin Regulates Seed Dormancy and Germination In Cereamentioning

confidence: 99%

Molecular Mechanisms Underlying Abscisic Acid/Gibberellin Balance in the Control of Seed Dormancy and Germination in Cereals

et al. 2018

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Seed dormancy is an adaptive trait that does not allow the germination of an intact viable seed under favorable environmental conditions. Non-dormant seeds or seeds with low level of dormancy can germinate readily under optimal environmental conditions, and such a trait leads to preharvest sprouting, germination of seeds on the mother plant prior to harvest, which significantly reduces the yield and quality of cereal crops. High level of dormancy, on the other hand, may lead to non-uniform germination and seedling establishment. Therefore, intermediate dormancy is considered to be a desirable trait as it prevents the problems of sprouting and allows uniformity of postharvest germination of seeds. Induction, maintenance, and release of seed dormancy are complex physiological processes that are influenced by a wide range of endogenous and environmental factors. Plant hormones, mainly abscisic acid (ABA) and gibberellin (GA), are the major endogenous factors that act antagonistically in the control of seed dormancy and germination; ABA positively regulates the induction and maintenance of dormancy, while GA enhances germination. Significant progress has been made in recent years in the elucidation of molecular mechanisms regulating ABA/GA balance and thereby dormancy and germination in cereal seeds, and this review summarizes the current state of knowledge on the topic.

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Physiological characteristics and related gene expression of after‐ripening on seed dormancy release in rice

Cited by 27 publications

References 53 publications

Identification and characterization of the rice pre‐harvest sprouting mutants involved in molybdenum cofactor biosynthesis

Identification and characterization of the rice pre‐harvest sprouting mutants involved in molybdenum cofactor biosynthesis

Proteomic Analysis Reveals Proteins Involved in Seed Imbibition under Salt Stress in Rice

Molecular Mechanisms Underlying Abscisic Acid/Gibberellin Balance in the Control of Seed Dormancy and Germination in Cereals

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