Physiological characteristics of Corynebacterium glutamicum as a cell factory under anaerobic conditions

Tsuge, Yota; Yamaguchi, A.

doi:10.1007/s00253-021-11474-w

Cited by 6 publications

(1 citation statement)

References 64 publications

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“…Further, we found an elevation of gram-negative and bio lm forming species. Species of genera Staphylococcus and Corynebacterium (gram-positive and facultative anaerobes [40][41][42] ) were shown to cause several bacterial infectious diseases in the human body 41 . Therefore, dysbiosis due to orofacial clefting may predispose for infections and woundhealing problems.…”

Section: Discussionmentioning

confidence: 99%

Orofacial Clefts Alter Early Life Oral Microbiome Maturation Towards Dysbiosis

Seidel

Strobel

Weider

et al. 2022

Preprint

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Orofacial clefts (OFC) present different phenotypes and severities with a postnatal challenge for oral microbiota development. In order to investigate the impact of OFC on oral microbiota, smear samples from 15 neonates with OFC and 17 neonates without OFC were collected from two oral niches (tongue, cheek) at two time points, i.e. at first consultation after birth (T0: Ø3d OFC group; Ø2d control group) and 4–5 weeks later (T1: Ø32d OFC group; Ø31d control group). Subsequently, the samples were processed and analyzed using next-generation sequencing. We detected a significant increase in alpha diversity and distinct phenotypes, e.g., anaerobic and gram-negative species from T0 to T1 in both groups. Further, we found that at T1 OFC neonates presented a significantly lower alpha diversity with lowest values for high cleft severity and significantly higher levels of Enterobacteriaceae (Citrobacter, Enterobacter, Escherichia-Shigella, Klebsiella), Enterococcus, Bifidobacterium, Corynebacterium, Lactocaseibacillus, Staphylococcus, Acinetobacter and Lawsonella compared to controls. Notably, neonates with unilateral and bilateral cleft lip (UCLP/BCLP) and palate presented similarities in beta diversity and a mixture with skin microbiota. However, significant differences were seen in neonates with cleft palate only compared to UCLP/BCLP with higher levels of anaerobic species. Our findings revealed an influence of OFC as well as cleft phenotype and severity on postnatal oral microbiota maturation.

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