Physiological characteristics of platelet/circulatory serotonin: study on a large human population

Jernej, Branimir; Banović, Miroslav; Čičin‐Šain, Lipa; Hranilović, Dubravka; Balija, Melita; Orešković, Darko; Folnegović‐Šmalc, Vera

doi:10.1016/s0165-1781(00)00129-3

Cited by 40 publications

(26 citation statements)

References 33 publications

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“…It is common to report platelet concentrations of 5-HT because platelets do not synthesize 5-HT, but they do exhibit transportermediated uptake of 5-HT from plasma, and once within the platelet, 5-HT is protected from monoamine oxidase activity. However, whole-blood and platelet concentrations have been shown to correlate well in a large population of humans, and measurement of blood concentrations avoids any errors introduced by alterations in platelet 5-HT transport (13)(14)(15). Calculations and data analysis.…”

Section: Methodsmentioning

confidence: 99%

Portal Serotonin Infusion and Glucose Disposal in Conscious Dogs

Moore

Geho²,

Lautz

et al. 2004

Diabetes

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P oor control of postprandial glycemia in type 2 diabetes is associated with elevated rates of all-cause mortality (1), making postprandial hyperglycemia an attractive pharmaceutical target. Individuals who have type 2 diabetes and receive monoamine oxidase inhibitors demonstrate more frequent episodes of hypoglycemia (2), and those who take selective serotonin reuptake inhibitors have improved glucose tolerance (3) compared with similar individuals who do not take these drugs. Moreover, intraperitoneal administration of serotonin (5-hydroxytryptamine [5-HT]) or its precursor 5-hydroxytryptophan has a hypoglycemic effect in mice (4). 5-HT accumulated in both the liver and the brain of the mice, leaving a question of which tissue was responsible for the hypoglycemia. However, in subsequent studies, the mice were also treated with carbidopa, an inhibitor of peripheral but not central aromatic amino acid decarboxylase. Neither hypoglycemia nor hepatic accumulation of 5-HT was observed in carbidopa-treated mice, although brain levels of 5-HT were even higher than in the absence of carbidopa. Thus, it seemed that hypoglycemia was related to the elevation of hepatic 5-HT (5). We hypothesized that 5-HT can reduce postprandial glycemia by enhancing NHGU and examined this hypothesis in conscious dogs in which the pancreatic hormones and hepatic glucose load (HGL) could be fixed. RESEARCH DESIGN AND METHODSAnimals and surgical procedures. Studies were carried out on 16 conscious 42-h-fasted mongrel dogs of either sex with a mean weight of 24 Ϯ 1 kg. Diet and housing were as previously described (6), and the protocol was approved by the Vanderbilt University Medical Center Animal Care Committee.Approximately 16 days before study, each dog underwent a laparotomy for placement of ultrasonic flow probes around the portal vein and the hepatic artery, as well as for insertion of silicone rubber catheters for sampling in a hepatic vein, the portal vein, and a femoral artery and for infusion into a splenic and a jejunal vein as described in detail elsewhere (6,7). Criteria for study were as previously described (6,7).On the morning of the study, catheters and flow probe leads were exteriorized from their subcutaneous pockets (6,7). The splenic and jejunal catheters were used for intraportal infusion of insulin (Eli Lilly & Co., Indianapolis, IN), glucagon (GlucaGen; Bedford Laboratories, Bedford, OH), and 5-HT (5-HT creatinine sulfate complex; Sigma, St. Louis, MO). Angiocaths (Deseret Medical, Sandy, UT) were inserted into three peripheral veins. Experimental design. Each experiment consisted of a 90-min equilibration period (Ϫ120 to Ϫ30 min), a 30-min basal period (Ϫ30 to 0 min), and a 270-min experimental period (0 to 270 min) divided into four subperiods (P1, 0 -90 min; P2, 90 -150 min; P3, 150 -210 min, and P4, 210 -270 min). At Ϫ120 min, a primed, continuous infusion of [3-3 H]glucose and a continuous infusion of indocyanine green dye (5 g ⅐ kg Ϫ1 ⅐ min Ϫ1 ) were begun in all dogs, with the exception of two that did not re...

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Section: Methodsmentioning

confidence: 99%

Portal Serotonin Infusion and Glucose Disposal in Conscious Dogs

Moore

Geho²,

Lautz

et al. 2004

Diabetes

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“…Evidence that serotonin concentrations vary by season is contradictory. Whereas one study [37] found low serotonin values in healthy volunteers during the summer as compared to the rest of the year, a large study also of healthy volunteers [38] (N = 500 approximately) found no substantial seasonal variations in WB serotonin. The SD in WB serotonin observed in the current study concur with those reported in other studies of healthy individuals [38].…”

Section: Discussionmentioning

confidence: 99%

Obesity, Whole Blood Serotonin and Sex Differences in Healthy Volunteers

Hodge¹,

Bunting²,

Carr³

et al. 2012

Obes Facts

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Background: Obesity is a growing problem throughout Europe, where the rate has more than doubled over the past 20 years. Reduced circulating serotonin may contribute to the development of obesity. This study aimed to explore associations between whole blood (WB) serotonin concentrations and anthropometric measures. Methods: Healthy adult volunteers (N = 68) gave whole blood samples for measurement of WB serotonin, and underwent BMI waist circumference (WC) and waist-to-hip ratio (WHR) assessment as well as DEXA (dual energy X-ray absorptiometry) scans for anthropometric parameters. Student’s t-tests determined differences in WB serotonin and anthropometric measures between sexes. Partial Pearson’s correlations were carried out on anthropometric measures and WB serotonin. Results: For the whole sample, WB serotonin was significantly negatively correlated with BMI, WC, WHR as well as android, gynoid and total % body fat. Analysis by sex showed significant negative correlations between WB serotonin and android, gynoid as well as total fat in males, but not in females. Conclusion: This dichotomy between the sexes implies that there may be sex differences in the way that serotonin interplays with the development of obesity and body fat distribution.

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“…Assays in platelet-poor plasma revealed that more than 99% of whole blood serotonin is contained in the platelet, 26 and whole blood serotonin correlates highly with measures of serotonin per unit number of platelets and serotonin per unit mass of platelet protein. 27 Whole-blood 5-HT was analyzed by high-pressure liquid chromatography (HPLC) with fluorometric detection. 28 Intra-assay and inter-assay coefficients of variance were 0.8 and 3.6%, respectively.…”

Section: Measurement Of Whole Blood Serotoninmentioning

confidence: 99%

Genome-wide association study identifies ITGB3 as a QTL for whole blood serotonin

et al. 2004

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Serotonin has been implicated in common disorders involving the central nervous, gastrointestinal, cardiovascular, and pulmonary systems. We describe the first genome-wide screen to identify quantitative trait loci (QTLs) influencing whole blood serotonin in 567 members of a single large pedigree, using a novel association-based mapping approach. We identified an association between the b3 integrin (ITGB3) Leu33Pro polymorphism on 17q21 and whole blood serotonin levels (P-value ¼ 9.8 Â 10 À5). This variant explained the evidence for linkage in this region when included as a covariate in the linkage analysis (change in LOD from 1.87 to 0.16), indicating that ITGB3 may be an important serotonin QTL.

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Physiological characteristics of platelet/circulatory serotonin: study on a large human population

Cited by 40 publications

References 33 publications

Portal Serotonin Infusion and Glucose Disposal in Conscious Dogs

Portal Serotonin Infusion and Glucose Disposal in Conscious Dogs

Obesity, Whole Blood Serotonin and Sex Differences in Healthy Volunteers

Genome-wide association study identifies ITGB3 as a QTL for whole blood serotonin

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