Physiological Characters and Serological Types of Haemolytic Streptococci of Groups B, C and G From Human Sources

Simmons, R. T.; Keogh, E. V.

doi:10.1038/icb.1940.16

Cited by 36 publications

(19 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The strains were uniform in their inability to ferment sorbitol and inulin. Our findings agree with those of Simmons and Keogh (1940) as to the biochemical characters of large-colony strains of human group-G streptococci, but we observed some differences between the biotype profiles of the human and animal strains, particularly in respect of the inability of many animal strains to ferment trehalose. Biberstein et al (1980) in biotyping a collection of group-G streptococci of canine origin reported that 167 of 206 strains failed to produce acid from this sugar.…”

Section: Discussionsupporting

confidence: 81%

See 1 more Smart Citation

Inhibitor production by group-G streptococci of human and of animal origin

Tagg

Wong

1983

Journal of Medical Microbiology

View full text Add to dashboard Cite

SUMMARY. Strains of group-G streptococci were tested by a "fingerprinting" method for the production of (P typing) and sensitivity to (S typing) inhibitory agents, and were biotyped. In the standard P-typing test, 28 of 50 strains of human origin, but none of 30 strains of animal origin, showed inhibitory activity. Of the human strains, 12 formed a bacteriocin that was active on group-A streptococci, including three (strains 12, I5 and 18) of the four streptococci of this group among the indicator strains. Sixteen other human strains inhibited the fourth group-A indicator (strain I7), and to a lesser extent strain 12, by lowering the pH of the typing medium. This acid-mediated inhibition was eliminated by testing on a medium containing calcium carbonate 0.5%; the 16 strains were then completely non-inhibitory, and the bacteriocinforming strains, the typing pattern of which had originally been 12,15, 17, 18, showed only inhibition attributable to the action of the bacteriocin. Nearly all group-A streptococci were sensitive to the group-G bacteriocin. The indicator strain I7 and several other members of M-type 28 were exceptions, but their resistance was not associated with the presence of R-antigen 28. Fifteen inhibitor-sensitivity patterns and 12 biotypes were identified among the strains; some of these tended to be associated with either a human or an animal origin. Neither S type nor biotype appeared to correlate with inhibitor production.

show abstract

Section: Discussionsupporting

confidence: 81%

“…Most clinical isolates are of the large-colony form, of which there appear to be at least three distinct serotypes (Simmons and Keogh, 1940). Several biotypes based on the .fermentation of lactose, trehalose and sorbitol have been described among group-G streptococci of canine origin (Biberstein et al, 1980).…”

Section: Introductionmentioning

confidence: 99%

Inhibitor production by group-G streptococci of human and of animal origin

Tagg

Wong

1983

Journal of Medical Microbiology

View full text Add to dashboard Cite

show abstract

“…of Bliss (1937) these two strains may or may not have been identical types as apparently according to Simmons & Keogh (1940) the 'minute-colony' strains of group G comprise more than one type, our 3 such strains none of which came from animal sources, were type Cuthell.…”

Section: Discussionmentioning

confidence: 99%

“…She proposed the designation type I in both groups for strains containing this type-specific antigen. Simmons & Keogh (1940) undertook the serological typing of group G strains of human origin. Of the 78 strains which they examined 70 were of the 'large-colony' type and 8 of the Types of group G streptococci 75 1 cocci which had been subjected to tryptic digestion in order to ensure stable suspensions.…”

Section: )mentioning

confidence: 99%

Animal Strains of Group G Streptococci and their Serological Typing

Laughton¹,

Davies²

1957

Journal of General Microbiology

View full text Add to dashboard Cite

SUMMARY: A brief review of group G streptococci and their typing is given with particular reference to canine strains. Two of the Australian type strains of Simmons & Keogh (1944)) were found to be in the group phase and useless for comparative purposes. Four types Kennett, Maxie, Airedale and R51/755 were clearly defined in addition to the Australian types Harrison and Cuthell by reciprocal absorption tests using slide agglutination. Fifty-four other strains of which 35 were of animal and 19 of human origin were examined for type. Of the 38 strains from animal sources, 33 belonged to one of the two types Kennett or Maxie, strains of which came exclusively from animal sources. Six of the human strains and one of the animal ones were untyped.Type R51/755 which had been found previously by Maxted (1949) t o possess the M28 antigen owed its type-specificity entirely to this antigen and this was not found to be present in any of the other types.The substances responsible for type-specificity in types Kennett, Maxie, and Airedale differed from those of types Harrison and Cuthell and like that of type R51/755 appeared to be protein in nature. The type-specific substances of types Airedale and Maxie had exactly the same characters as the M28 antigen in being sensitive to peptic but resistant to tryptic digestion. The protein antigen of type Kennett differed from the other three protein type-specific substances in showing only moderate resistance to tryptic digestion.

show abstract

“…The opacity appeared to be due to corn starch. Streptococcus agalactiae is described in the literature as negative in starch hydrolysis tests (Buchanan & Gibbons, 1974) and acid production from starch tests (Simmons & Keogh, 1940). This paper describes comparative results for S. agalactiae and other bacteria in simple tests for the degradation of polysaccharides.…”

mentioning

confidence: 99%

Polysaccharase Activity in Streptococcus agalactiae (Group B Streptococci)

Davis¹,

Pham²,

Triscott³

1982

Microbiology

View full text Add to dashboard Cite

1381 ~~ ~~Of 300 recently isolated strains of Streptococcus agalactiae from human sources, 97 % degraded starch. Representative strains also degraded glycogen, pullulan, amylopectin and amylose. The polysaccharase activity is easily detected by clearing around growth on Columbia agar base medium. The activity is weaker than that of some S. pyogenes strains, and it does not appear to produce fermentable products but is inhibited by the presence of easily used sugars. I N T R O D U C T I O NWhile working with Streptococcus agalactiae strains we noticed that growth on Columbia agar base, without blood, resulted in clearing of the slightly opaque medium. The opacity appeared to be due to corn starch. Streptococcus agalactiae is described in the literature as negative in starch hydrolysis tests (Buchanan & Gibbons, 1974) and acid production from starch tests (Simmons & Keogh, 1940). This paper describes comparative results for S. agalactiae and other bacteria in simple tests for the degradation of polysaccharides. M E T H O D SBacteria. Three hundred strains of S. agalactiae recently isolated from human sources were maintained by freeze-drying. Five serotype strains of S. agalactiae were taken from lyophilized stock in the University of Queensland Microbiology Department Culture Collection (UQM 1737, 1738, 1739, 1740 and 1744 representing serotypes Ia, Ib, Ic, I1 and 111, respectively). The identity of all S. agalactiae strains was confirmed serologically and by CAMP and hippurase reactions. Ten strains of Group D streptococci freshly isolated from human faeces and sewage on bile-aesculin agar were identified serologically. Ten strains of viridans streptococci were freshly isolated from human saliva on mitis-salivarius agar by selecting colonies of the Streptococcus sanguis and S . salivarius type. Escherichia coli (UQM 70), Enterobacter aerogenes (UQM 427), Streptococcus pyogenes (UQM 1897), Corynebacterium diphtheriae var. gravis (UQM 890), Corynebacterium diphtheriae var. intermedius (UQM 896) and Listeria monocytogenes (UQM 3691) were obtained from the University of Queensland Microbiology Department Culture Collection. All the bacteria were maintained by subculture on 5 % horse blood agar and in cooked-meat medium (Oxoid CM439). Substrate degradation tests(a) Agar plate method. Maize starch, potato starch, soluble starch, oyster glycogen, dextrin (BDH), amylose, amylopectin and pullulan (Sigma) were added to 100 ml lots of blood agar base no. 2 (Oxoid CM271) to give 0 -2 % (w/v) final concentration, sterilized at 12 1 *C for 15 min, mixed well and poured as top layers on prepoured layers of 0.9% (w/v) saline agar in 9 cm Petri dishes; 100 ml of substrate medium gave 12 plates. Controls without substrate and double-layer plates of Columbia agar base (CAB, Gibco) were also prepared. The plates were spot inoculated (four strains per plate) with about 30 pl of 24 h cultures in Todd-Hewitt broth (THB, Gibco), delivered with Pasteur pipettes. All of the 33 1 strains were tested on CAB. Nine strains of S. agalactiae (i...

show abstract

Physiological Characters and Serological Types of Haemolytic Streptococci of Groups B, C and G From Human Sources

Cited by 36 publications

References 1 publication

Inhibitor production by group-G streptococci of human and of animal origin

Inhibitor production by group-G streptococci of human and of animal origin

Animal Strains of Group G Streptococci and their Serological Typing

Polysaccharase Activity in Streptococcus agalactiae (Group B Streptococci)

Contact Info

Product

Resources

About