Soybean is a legume that can be cultivated in various types of agroecosystems such as lowlands, dry land, and swamps with various types of soil. This research aims to analyze the effect of mycorrhizal application on the growth and yield of soybeans planted in various agroecosystems. The research was designed with a two-factor randomized block design. Location of soybean planting as the first factor consists of four soybean development locations in Takalar Regency, South Sulawesi, namely: soybean cultivation in dry land, lowland in Galesong subdistrict, soybean cultivation in dryland, and lowland in South Galesong subdistrict. Application of mycorrhizal is the second factor which consists of two levels: no application, and mycorrhizae application. The results showed that the location of planting had a significant effect on soybean production, where soybean cultivation in lowlands resulted in significantly higher soybean production. The mycorrhizal application treatment obtained significantly higher soybean growth and production. The results of the analysis of soil chemical properties show that the total N content of soil in lowland land is moderate, P-available has low status, and low C-organic status. Galesong dry land showed a high total N content of 0.59%, but available P and organic C are very low.