Physiological concentration of amino acids regulates insulin-like-growth-factor-binding protein 1 expression

Jousse, Céline; Bruhat, Alain; Ferrara, Marc; Fafournoux, Pierre

doi:10.1042/bj3340147

Cited by 86 publications

(50 citation statements)

References 45 publications

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“…Direct, because EAAs stimulate the protein synthesis [31] and inhibit proteolysis at the same time [32]. Indirect, because EAAs boost protein formation by inducing protein production, and also increase the biological activity of insulin-like growth factor (IGF-I) [33]. The documented evidence that a three-month supplementation of 7.5 g/day of EAAs in older women increases base IGF-I expression [34] supports this hypothesis.…”

Section: A) Eaas and Physical Activitysupporting

confidence: 61%

Essential amino acid supplementation in patients with severe COPD: a step towards home rehabilitation

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Testa

Aquilani

et al. 2015

Monaldi Arch Chest Dis

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Background. Pulmonary Rehabilitation (“Rehabilitation”) can improve both lung function and quality of life in patients suffering from chronic obstructive pulmonary disease (COPD) even if only a very small proportion of patients have access to Rehabilitation. Supplementation of Essential Amino Acids (EAAs) might allow COPD patients to achieve some typical Rehabilitation outcomes such as a better physical performance and an improved health status. Methods. 88 COPD out-patients (GOLD class 3-4) with a body mass index (BMI) <23 Kg/m2 were randomised to receive EAAs (n=44) or placebo (n=44) for twelve weeks. Primary outcome measures were changes in both physical activities in daily life (measured by Sense Wear Armband in terms of mean steps walked in one week) and in quality of life (measured by the St George’s Respiratory Questionnaire, SGRQ). Results. After 12 weeks, the physical performance was significantly increased vs baseline only in patients who received EAAs (1140.33 ± 524.69 and 638.68 ± 662.1 steps/day, respectively; p=0.02), being also the comparison vs the placebo group highly significant (p=0.003). Similarly, the SGRQ score improved significantly only in EAA patients (69.35 ± 9.51 vs baseline 72.04 ± 8.62; p<0.01), and changes were significantly different from those measured in the placebo group (p<0.001). Furthermore, when compared to those who received placebo, EAAs patients significantly increased their fat-free mass (p=0.04), muscle strength (p<0.01), saturation of oxygen (p=0.05), serum albumin (p<0.001), and also ameliorated their original cognitive dysfunction (p=0.02). Conclusions. Oral supplementation with EAAs contribute to improve the daily-life performance in domiciliary severe COPD patients who can not enter any Rehabilitation programme, together with their quality of life; nutritional and cognitive status, and muscle strength.

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Section: A) Eaas and Physical Activitysupporting

confidence: 61%

Essential amino acid supplementation in patients with severe COPD: a step towards home rehabilitation

Negro

Testa

Aquilani

et al. 2015

Monaldi Arch Chest Dis

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“…In recent years, evidence has accumulated that amino acids play an important role in controlling gene expression (30,32). Nevertheless, the molecular mechanisms involved in the amino acid regulation of mammalian gene expression have not been elucidated to date.…”

Section: Discussionmentioning

confidence: 99%

Amino Acids Control Mammalian Gene Transcription: Activating Transcription Factor 2 Is Essential for the Amino Acid Responsiveness of the CHOP Promoter

Bruhat¹,

Jousse²,

Carraro³

et al. 2000

Molecular and Cellular Biology

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174

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In mammals, plasma concentration of amino acids is affected by nutritional or pathological conditions. It has been well established that nutrients, and particularly amino acids, are involved in the control of gene expression. Here we examined the molecular mechanisms involved in the regulation of CHOP (a CCAAT/enhancerbinding protein [C/EBP]-related gene) expression upon amino acid limitation. We have previously shown that regulation of CHOP mRNA expression by amino acid concentration has both transcriptional and posttranscriptional components. We report the analysis of cis-and trans-acting elements involved in the transcriptional activation of the human CHOP gene by leucine starvation. Using a transient expression assay, we show that a cis-positive element is essential for amino acid regulation of the CHOP promoter. This sequence is the first described that can regulate a basal promoter in response to starvation for several individual amino acids and therefore can be called an amino acid response element (AARE). In addition, we show that the CHOP AARE is related to C/EBP and ATF/CRE binding sites and binds in vitro the activating transcription factor 2 (ATF-2) in starved and unstarved conditions. Using ATF-2-deficient mouse embryonic fibroblasts and an ATF-2-dominant negative mutant, we demonstrate that expression of this transcription factor is essential for the transcriptional activation of CHOP by leucine starvation. Altogether, these results suggest that ATF-2 may be a member of a cascade of molecular events by which the cellular concentration of amino acids can regulate mammalian gene expression.

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“…Straus (112) showed that single amino acid limitation of hepatoma cells in culture also resulted in an increase in IGFBP-1 mRNA. Jousse et al (57) investigated the expression of IGF-I, IGF-II, and IGFBP-1 in response to amino acid limitation in cultured cells and showed that IGFBP-1 mRNA and protein content was increased significantly, whereas the expression of the IGF-1 and IGF-II were either unaffected or slightly reduced. These observations appear to conflict with the observations of Entingh et al (26), who reported that the activation of the CHOP gene by amino acid deprivation of fibroblasts requires the presence of IGF-1.…”

Section: Insulin-like Growth Factor Binding Protein-1-mentioning

confidence: 99%

NUTRITIONAL CONTROL OF GENE EXPRESSION: How Mammalian Cells Respond to Amino Acid Limitation

et al. 2005

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The amino acid response (AAR) pathway in mammalian cells is designed to detect and respond to amino acid deficiency. Limiting any essential amino acid initiates this signaling cascade, which leads to increased translation of a "master regulator," activating transcription factor (ATF) 4, and ultimately, to regulation of many steps along the pathway of DNA to RNA to protein. These regulated events include chromatin remodeling, RNA splicing, nuclear RNA export, mRNA stabilization, and translational control. Proteins that are increased in their expression as targets of the AAR pathway include membrane transporters, transcription factors from the basic region/ leucine zipper (bZIP) superfamily, growth factors, and metabolic enzymes. Significant progress has been achieved in understanding the molecular mechanisms by which amino acids control the synthesis and turnover of mRNA and protein. Beyond gaining additional knowledge of these important regulatory pathways, further characterization of how these processes contribute to the pathology of various disease states represents an interesting aspect of future research in molecular nutrition.Keywords nutrient starvation; metabolite control; protein metabolism; transcription; mRNA stability * ABBREVIATIONS: AAR, amino acid response (pathway); AARE, amino acid response element; ARE, AU-rich elements; ASNS, asparagine synthetase; Cdk, cyclin-dependent kinase; CdkI, cyclin-dependent kinase inhibitor; C/EBP, CCAAT-enhancer binding protein; ChIP, chromatin immunoprecipitation; CHOP, C/EBP homology protein/growth arrest and DNA damage 153; EMSA, electrophoresis mobility shift analysis; mTOR, mammalian target of rapamycin; NSRE, nutrient-sensing response element; UPR, unfolded protein response.

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Physiological concentration of amino acids regulates insulin-like-growth-factor-binding protein 1 expression

Cited by 86 publications

References 45 publications

Essential amino acid supplementation in patients with severe COPD: a step towards home rehabilitation

Essential amino acid supplementation in patients with severe COPD: a step towards home rehabilitation

Amino Acids Control Mammalian Gene Transcription: Activating Transcription Factor 2 Is Essential for the Amino Acid Responsiveness of the CHOP Promoter

NUTRITIONAL CONTROL OF GENE EXPRESSION: How Mammalian Cells Respond to Amino Acid Limitation

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