Physiological control of pheromone production inChoristoneura fumiferana andC. Rosaceana

Abstract

The diel periodicity of calling behavior and pheromone production are synchronous in virgin females of both Choristoneura fumiferana and C. rosaceana (Lepidoptera: Tortricidae). Newly emerged females decapitated prior to scotophase produced no or very little pheromone 24 h later. However, injection of PBAN or Br‐SEG homogenates, obtained from donors of the same or the other species, stimulated pheromone production to normal levels. Transection of the ventral nerve cord (VNC) or extirpation of the terminal abdominal ganglion (TAG) did not affect pheromone production in control females. Similarly, injections of PBAN or Br‐SEG homogenates into decapitated females reactivated pheromone production to normal levels, whether or not the VNC was intact or the TAG present. Furthermore, octopamine was not effective in stimulating pheromone production in decapitated females. Taken together, these results indicate that the regulation of pheromone production is not neurally mediated in either Choristoneura species. However, there was no evidence that hemolymph collected from pheromone‐producing females contained pheromonotropic activity. Similarly, isolated glands incubated with PBAN did not produce pheromone. The presence of the bursa copulatrix was required to produce pheromone in both tortricids as production was not restored in decapitated bursa‐less females injected with PBAN or a Br‐SEG homogenate. However, an extract of the bursa copulatrix did not elicit pheromonotropic activity in decapitated females or incubated glands of either species. The bursa copulatrix is only involved in pheromone production of some species of tortricids but our results do not support the current explanation for such interspecific differences. We postulate that the relative importance of a bursa factor may be related to the evolution of different desaturation systems used for pheromone biosynthesis in the Tortricidae. Arch. Insect Biochem. Physiol. 42:253–265, 1999. © 1999 Wiley‐Liss, Inc.

The sublethal effects of tebufenozide on the precopulatory and copulatory activities of Choristoneura fumiferana and C. rosaceana

“…29). Observations of the calling behavior in SBW began 2 h (from 07.00 to 17.00 hours) before the onset of the scotophase, as reported by Delisle et al. (1999), whereas observations of OBL began at lights off (09.00 to 17.00 hours) (Delisle, 1992).…”

Identification of a new member of PBAN family and immunoreactivity in the central nervous system from Adoxophyes sp. (Lepidoptera: Tortricidae)

“…Pheromone production of tortricid moths was stimulated by PBAN as other moths (Jurenka et al, 1991). In addition to PBAN, an effect from the bursa copulatrix is involved in pheromone biosynthesis in some species of tortricidae, Argyrotaenia velutinana, Choristoneura fumiferana, and Choristoneura rosaceana (Jurenka et al, 1991;Delisle et al, 1999).…”

Mate‐finding allee effect in spruce budworm population dynamics

“…The extracts were subsequently analyzed using gas chromatography (GC) (Delisle & Royer, 1994). The two pheromone gland components analyzed were (E)-11tetradecenyl aldehyde (E11-14:Ald) (the major component emitted by spruce budworm females) and (E)-11-tetradecenyl acetate (E11-14:Ac) (precursor to the aldehyde component) (Silk et al, 1980;Delisle et al, 1999). A total of 71 individual glands were analyzed from laboratory-reared females and 79 from wild females.…”