Physiological Disposition of Heparin.

Eiber, Harold B.; Danishefsky, I.; Borrelli, F

doi:10.3181/00379727-98-24147

Cited by 9 publications

(2 citation statements)

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“…Loomis (17) employing 3sS-heparin in rats showed that the coagulation time decreased receive heparin but to which 3H-heparin was added in vitro; conditions were similar to those stated in 3 a. to normal when 20Vo of the radioactivity had emerged in the urine of intact animals. Eiber et al (8) using dogs observed that 70Vo of. the 3sS-heparin dose was excreted in 48 hrs; after 24 hrs 28Vo of.…”

Section: Discussionmentioning

confidence: 98%

Heparin Excretion in Intact and Hepatectomized Rats

1981

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SummaryMetabolism and kinetics of 3H-heparin were compared in intact and hepatectomized rats. Rats were divided into three groups: 1) intact rats with biliary fistulas and cystostomies 2) intact rats with only cystostomies and 3) hepatectomized rats with cystostomies. Radioactivity in blood, bile and urine besides anticoagulant activity in blood and urine were examined. In addition, column chromatography of urine was used to isolate possible metabolites. Seventy percent and 80% of the radioactive dose was found in the urine of intact rats at 24 hr and 48 hr. Close to 5% of the radioactivity was found in bile or rats with a biliary fistula after 48 hr. The APTT declined to near normal values at 1 hr whether rats had a biliary fistula or not. In contrast, only 25 % of the radioactivity could be excreted into the urine of hepatectomized rats in 24 hr; the APTT did not decline as fast and at 5 hr, it was still 100 seconds. Only one radioactive component could be isolated on chromatography from all urines of these animals and appears to be similar to the original heparin. Thus, the liver has an important role to play in regulating the anticoagulant effects and excretion of heparin.

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Section: Discussionmentioning

confidence: 98%

Heparin Excretion in Intact and Hepatectomized Rats

1981

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“…Loomis, 1959Loomis, , 1961, the sequence of events involved in the disposal of the injected polymer is still not fully understood. Up to the present, studies on the metabolic fate of heparin have been based on observations made after the injection of unlabelled biosynthetic preparations of the polymer (Wilander & Holmgren, 1938;Wilander, 1939;Jacques, 1939;Reinert & Winterstein, 1939;Copley & Schendorf, 1941;Piper, 1947; Jaques, Napke & Levy, 1953), of preparations labelled biosynthetically with radioactive sulphate (Eiber, Danishefsky & Borelli, 1958;Danishefsky & Eiber, 1959;Loomis, 1961;Day, Green & Robinson, 1962) and of semi-chemically synthesized material prepared by the selective N-resulphation with trimethylamine-[35S]sulphur trioxide of N-desulphated commercial heparin (Levy & Petracek, 1962). The earlier reports dealt mainly with the urinary excretion of the apparently unchanged polymer, but in the later work attention has been given to the tissue distribution of radioactivity and to the appearance of inorganic [35S]sulphate, detectable in the tissues or in the urine, after the injection of the 35S-labelled materials.…”

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Preparation of potassium 2-deoxy-2-[35S]sulphoamino-d-glucose

Lloyd¹,

Wusteman²,

Tudball³

et al. 1964

Biochemical Journal

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2. The isomers in the major dienoic acid fraction had c8-c8 non-conjugated configurations, and the double bonds were largely at C-11 or C-12 and C-15 or C-16. 3. The monoenoic acids formed were largely trans, with the double bond predominantly at C-13 or C-14. 4. Rapid hydrogenation of [1-14C]linoleic acid and [1-14C]oleic acid occurred in the artificial rumen. The former gave a trans monoenoic acid as an intermediary. 5. The tran8 C18 monoenoic acids passing from the rumen were almost quantitatively absorbed in the ileum. 6. The unsaturated C18 acids present in the ileum digesta were hydrogenated in the caecum and colon, so that nearly all the acids in the excreta were saturated. 7. The hydrogenation of [1-'4C]linoleic acid by caecal and colon contents resulted again in the formation of substantial amounts of trans C18 monoenoic acids. T. W. S. was in receipt of a Fellowship from the International Atomic Energy Agency during the course of this work. The Agency is also thanked for a grant towards the purchase of radioactive substrates and measuring equipment. Mr N. Huskisson and Mr N. Clarke are thanked for their valuable assistance.

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