1997
DOI: 10.1111/j.1525-1594.1997.tb00733.x
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Physiological Effect of Polyethylene Glycol Conjugation on Stroma‐Free Bovine Hemoglobin in the Conscious Dog After Partial Exchange Transfusion

Abstract: This study was designed to determine the effect of polyethylene glycol (PEG) conjugation on stroma-free bovine hemoglobin. This was accomplished by examining the effects of unmodified stroma-free bovine hemoglobin (bovine Hb), PEG modified bovine hemoglobin (PEGHb) and dextran 70 on heart rate (HR), mean arterial pressure (MAP), gross renal morphology, blood chemistry, and hemoglobinuria development in conscious beagle dogs following a 30% exchange transfusion. Dogs were implanted with telemetric blood pressur… Show more

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Cited by 27 publications
(12 citation statements)
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“…CO has antiinflammatory (Ozaki et al, 2012) and vasodilator effects (Leffler et al, 2011). Conjugation with PEG increases the molecular radius to minimize extravasation, increases the circulating half-life of the product (Conover et al, 1997;Conover et al, 1996;Tsai et al, 2006), and increases plasma viscosity (Wettstein et al, 2003). Plasma viscosity may be important for maintaining shear stress-induced NO production by the endothelium and thus may be another mechanism, in addition to the CO, to promote vasodilation (Tsai et al, 2005).…”
Section: Microvascular Researchmentioning
confidence: 95%
“…CO has antiinflammatory (Ozaki et al, 2012) and vasodilator effects (Leffler et al, 2011). Conjugation with PEG increases the molecular radius to minimize extravasation, increases the circulating half-life of the product (Conover et al, 1997;Conover et al, 1996;Tsai et al, 2006), and increases plasma viscosity (Wettstein et al, 2003). Plasma viscosity may be important for maintaining shear stress-induced NO production by the endothelium and thus may be another mechanism, in addition to the CO, to promote vasodilation (Tsai et al, 2005).…”
Section: Microvascular Researchmentioning
confidence: 95%
“…Changes in the plasma concentration of PEG-Hb™ after a top-load infusion (25 mL/kg) was examined in rats and rabbits, and the findings showed that the mean half-life was approximately 17 h and 43 h in rats and rabbits, respectively [61,63]. In addition, PEG-Hb™ was shown to have a half-life of 15 h and 58 h in rat and dog models of 30% blood volume exchange transfusion [62,64]. Furthermore, PEG-Hb™ showed a longer half-life than native Hb in a canine hemorrhagic shock model [65] These long half-lives, as summarized in Table 3, indicate that PEG modification can prevent excretion via glomerular filtration due to the increase in overall molecular weight.…”
Section: Acellular Type Hbocsmentioning
confidence: 99%
“…Hypervolemic exchange transfusion of larger polymers of Hb that extravasate much less and do not impair endothelium-dependent dilation or cause hypertension (Matheson et al 2002) has also been found to reduce infarct volume from experimental stroke (Bobofchak et al 2003, Nemoto et al 2006, Mito et al 2009). Analogous to polymeric Hb, polyethylene glycol-conjugated Hb (PEG-Hb) has an increased molecular radius and does not readily extravasate or cause hypertension (Conover et al 1996, Conover et al 1997, Tsai et al 2006, Young et al 2007). An additional benefi t is that conjugation of the Hb molecule surface with PEG reduces immunogenicity, increases oncotic pressure, and increases plasma viscosity (Nucci et al 1996, Intaglietta 1999, Wettstein et al 2003.…”
Section: Introductionmentioning
confidence: 99%