Physiological Effects and Transcriptomic Analysis of sbGnRH on the Liver in Pompano (Trachinotus ovatus)

Abstract

Pompano (Trachinotus ovatus) is one of the important economic marine fishes in the south coast of China. At present, the research on the basic biology of pompano is relatively weak, which has seriously affected the development of this economic important fish. The liver is an important digestive and metabolic organ of fish which plays an important regulatory role in its growth and development. It is necessary to clarify the effects of sea bream gonadotropin releasing hormone (sbGnRH) on liver physiology and metabolic enzyme activity. The effects of sbGnRH peptides (10 ng/gbw) on the physiological and biochemical indices and metabolic enzyme activities of pompano liver were studied. It was found that after injection of 10 ng/gbw sbGnRH peptides, the contents of albumin, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, glucose, creatine kinase, iron, magnesium, aspartate aminotransferase, alanine aminotransferase and creatinine increased, while of cholesterol and calcium contents decreased. The activities of amylase, lipase, pyruvate kinase, acyl CoA oxidase, superoxide dismutase, phospholipid hydroperoxide glutathione peroxidase, catalase, glucose-6-phosphate dehydrogenase, fatty acid synthase and lipoprotein lipase increased, while the activities of malic enzyme, carnitine acyl, carnitine translocation, acetyl CoA carboxylase and malondialdehyde decreased. Three hours after the injection of different concentrations of sbGnRH peptides (0 and 10 ng/gbw), the transcriptome sequences of the two groups of livers were sequenced. After quality control and removal of some low-quality data, clean reads of 21,283,647、19,427,359、21,873,990、21,732,174、23,660,062 and 21,592,338 were obtained respectively. In this study, 99 genes were screened and identified as differentially expressed genes, including 77 up-regulated genes and 22 down-regulated genes. According to the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) pathway analyses, these pathways and the typical genes involved can be divided into cellular processes, environmental information processing, genetic information processing, diseases, metabolism and organismal systems. The results from this study provide a the oretical basis for studying the effects of sbGnRH on the physiology, biochemistry and metabolic enzyme activities of liver in pompano.

Physiological responses of Catfish liver to katuk extract, turmeric powder, and vitamin C supplemented in the diet during ovarium development

“…Physiological parameters and liver histology are indications of permanent destruction in the exposed shes so that it implies the importances of liver health in sh (Topic Popovic et al, 2023). Liver is also digestive and metabolism organs that are important in the growth and development of teleost shes (Ren et al, 2022) and oviparous animals. By using turmeric powder, katuk extract, and vitamin C will improve liver performances to produce vitellogenin, that stimulate the disruption of hepatocytes.…”

Identification of Insulin-like Growth Factor (IGF) Family Genes in the Golden Pompano, Trachinotus ovatus: Molecular Cloning, Characterization and Gene Expression

“…The golden pompano, Trachinotus ovatus , is an economically important saltwater fish in China, belonging to the Carangidae family [ 1 , 2 ]. Due to its fast growth and adaptability for cage culture, it is a suitable fish for aquaculture [ 2 ]. The annual production of golden pompano has reached 240,000 tons [ 2 ].…”

“…Due to its fast growth and adaptability for cage culture, it is a suitable fish for aquaculture [ 2 ]. The annual production of golden pompano has reached 240,000 tons [ 2 ]. T. ovatus is a highly sought-after fish species due to its mild, sweet flavor and firm, white flesh.…”

“…It is also known for its high nutritional value, being an excellent source of omega-3 fatty acids, vitamins, and minerals. It is an excellent source of essential nutrients and can help to improve overall health and reduce the risk of certain diseases [ 2 ]. For these reasons, it is becoming increasingly popular as a dietary supplement and a source of nutrition for those looking to improve their health and well-being [ 2 ].…”

“…It is an excellent source of essential nutrients and can help to improve overall health and reduce the risk of certain diseases [ 2 ]. For these reasons, it is becoming increasingly popular as a dietary supplement and a source of nutrition for those looking to improve their health and well-being [ 2 ]. In recent years, the golden pompano fish has become the subject of considerable research due to its potential health benefits, particularly regarding insulin-like growth factors (IGFs).…”