Physiological Effects of GLT1 Modulation in Saccharomyces cerevisiae Strains Growing on Different Nitrogen Sources

Brambilla, Marco; Adamo, Giusy Manuela; Frascotti, Gianni; Porro, Danilo; Branduardi, Paola

doi:10.4014/jmb.1508.08002

Cited by 2 publications

(2 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It seems that GOGAT constitute an ancillary pathway, supplying low but continuous glutamate production, even in the presence of other glutamate pathways, suggesting that a high intracellular glutamate concentration may be needed for optimal growth, mainly under conditions in which carbon and nitrogen are limiting ( Valenzuela et al, 1998 ). GLT1 knockout evaluation showed that GOGAT does not significantly influence cellular physiology, corroborating its auxiliary characteristic in glutamate synthesis ( Brambilla et al, 2016 ), though GOGAT non-expression yeasts accumulated more ROS, when treated with hydrogen peroxide.…”

Section: Inteins In Candida Speciesmentioning

confidence: 87%

Evolution and Application of Inteins in Candida species: A Review

et al. 2016

View full text Add to dashboard Cite

Inteins are invasive intervening sequences that perform an autocatalytic splicing from their host proteins. Among eukaryotes, these elements are present in many fungal species, including those considered opportunistic or primary pathogens, such as Candida spp. Here we reviewed and updated the list of Candida species containing inteins in the genes VMA, THRRS and GLT1 and pointed out the importance of these elements as molecular markers for molecular epidemiological researches and species-specific diagnosis, since the presence, as well as the size of these inteins, is polymorphic among the different species. Although absent in Candida albicans, these elements are present in different sizes, in some environmental Candida spp. and also in most of the non-albicans Candida spp. considered emergent opportunistic pathogens. Besides, the possible role of these inteins in yeast physiology was also discussed in the light of the recent findings on the importance of these elements as post-translational modulators of gene expression, reinforcing their relevance as alternative therapeutic targets for the treatment of non-albicans Candida infections, because, once the splicing of an intein is inhibited, its host protein, which is usually a housekeeping protein, becomes non-functional.

show abstract

Section: Inteins In Candida Speciesmentioning

confidence: 87%

Evolution and Application of Inteins in Candida species: A Review

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Nitrogen source is the major material used to maintain cell growth and participate in the synthesis of nitrogen-containing compounds (Parente et al 2018 ). In microbial manufacturing, microorganisms can utilize different kinds of nitrogen sources, such as amino acids (Brambilla et al 2016 ), ammonium ions (Mendes-Ferreira et al 2004 ), peptides (Kevvai et al 2016 ), urea (Yang et al 2021 ) and so on. Considering the cost and utilization, nitrogen sources commonly used in microbial fermentation mainly include (NH 4 ) 2 SO 4 (Mohanrasu et al 2020 ), (NH 4 ) 2 HPO 4 (Sun et al 2016 ), NH 4 Cl (Ryu et al 2020 ), yeast extract (Goksungur and Guvenc 1997 ), peptone (Sun et al 2021 ), corn steep liquor (Shen et al 2016 ) and so on.…”

Section: Application Of Traditional Methods In the Process Optimizati...mentioning

confidence: 99%

Current advances for omics-guided process optimization of microbial manufacturing

Wan

Liu

Sun

et al. 2023

Bioresour. Bioprocess.

View full text Add to dashboard Cite

Currently, microbial manufacturing is widely used in various fields, such as food, medicine and energy, for its advantages of greenness and sustainable development. Process optimization is the committed step enabling the commercialization of microbial manufacturing products. However, the present optimization processes mainly rely on experience or trial-and-error method ignoring the intrinsic connection between cellular physiological requirement and production performance, so in many cases the productivity of microbial manufacturing could not been fully exploited at economically feasible cost. Recently, the rapid development of omics technologies facilitates the comprehensive analysis of microbial metabolism and fermentation performance from multi-levels of molecules, cells and microenvironment. The use of omics technologies makes the process optimization more explicit, boosting microbial manufacturing performance and bringing significant economic benefits and social value. In this paper, the traditional and omics technologies-guided process optimization of microbial manufacturing are systematically reviewed, and the future trend of process optimization is prospected.

show abstract

Physiological Effects of GLT1 Modulation in Saccharomyces cerevisiae Strains Growing on Different Nitrogen Sources

Cited by 2 publications

References 34 publications

Evolution and Application of Inteins in Candida species: A Review

Evolution and Application of Inteins in Candida species: A Review

Current advances for omics-guided process optimization of microbial manufacturing

Contact Info

Product

Resources

About