Physiological effects of high-P50 erythrocyte transfusion on piglets

Teisseire, B; Ropars, C.; Vallez, Marie‐Odile; Herigault, R. A.; Nicolau, Claude

doi:10.1152/jappl.1985.58.6.1810

Cited by 49 publications

(40 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This result is in agreement with previous observations (2,3) following the introduction of RBCs with right-shifted Hb-O 2 affinity, where the benefit from enhanced oxygenation due to the facilitated release of O 2 is opposed by vasoconstriction and reduced blood flow. MAP in general was significantly reduced by hemodilution, but to a lesser degree in animals exchange transfused with RBCs of lower Hb-O 2 affinity (Table 1).…”

Section: Systemic Versus Microvascular Responsesupporting

confidence: 93%

“…Few methods have been reported to raise P50 in vivo, where the induced P50 increases were always modest and/ or sustained for only a short time. Teisseire and coworkers reported a method for increasing P50 in anesthetized piglets using inositol hexaphosphate (InsP6) (2,3). InsP6 is an effective Hb allosteric effector (4,5); however, since it cannot diffuse through the erythrocytic membrane, it has to be introduced into erythrocytes using a reverse osmoticlysis process or electroporation (6,7).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Modulation of Perfusion and Oxygenation by Red Blood Cell Oxygen Affinity during Acute Anemia

Cabrales¹,

Tsai²,

Intaglietta³

2008

Am J Respir Cell Mol Biol

View full text Add to dashboard Cite

Responses to exchange transfusion using red blood cells (RBCs) with modified hemoglobin (Hb) oxygen (O 2 ) affinity were studied in the hamster window chamber model during acute anemia to determine its role on microvascular perfusion and tissue oxygenation. Allosteric effectors were introduced in the RBCs by electroporation. Inositol hexaphosphate (IHP) and 5-hydroxymethyl-2-furfural (5HMF) were used to decrease and increase Hb-O 2 affinity. In vitro P50s (partial pressure of O 2 at 50% Hb saturation) were modified to 10, 25, 45, and 50 mm Hg (normal P50 is 32 mm Hg). Allosteric effectors also decreased the Hill coefficient. Anemic condition was induced by isovolemic hemodilution exchanges using 6% dextran 70 kD to 18% hematocrit (Hct). Modified RBCs (at 18% Hct in 5% albumin solution) were infused by exchange transfusion of 35% of blood volume. Systemic parameters, microvascular perfusion, capillary perfusion (functional capillary density, FCD), and microvascular PO 2 levels were measured. RBcs with P50 of 45 mm Hg increased tissue PO 2 and decreased O 2 delivery (DO 2 ) and extraction (VO 2 ) and RBCs with P50 of 60 mmHg reduced FCD, microvascular flow, tissue PO 2 , DO 2 and VO 2 . Erythrocytes with increased Hb-O 2 affinity maintained hemodynamic conditions, DO 2 and decreased tissue PO 2 . This study shows that in an anemic condition, maximal tissue PO 2 does not correspond to maximal DO 2 and VO 2 .

show abstract

Section: Systemic Versus Microvascular Responsesupporting

confidence: 93%

mentioning

confidence: 99%

Modulation of Perfusion and Oxygenation by Red Blood Cell Oxygen Affinity during Acute Anemia

Cabrales¹,

Tsai²,

Intaglietta³

2008

Am J Respir Cell Mol Biol

View full text Add to dashboard Cite

show abstract

“…Except in cases of the most severe cardio-pulmonary insufficiency, arterial P02 will be considerably higher than that; but we may also predict that as the amount of the right shift increases, the critical arterial P02 required to give adequate saturation in the lungs will also increase, conceivably to the point where normal air breathing would be inadequate. This point is illustrated for human blood in Figure 4 (calculated from Teisseire et al, 1985). As P50 rises, so does the P02 required to give adequate saturation in the lungs (in this case plotted arbitrarily for 80, 90 and 95%) so that if we wish to exploit these effects clinically, careful consideration must be given to the arterial P02 of the patient and whether supplemental oxygen might be required to maintain an acceptable level of haemoglobin saturation.…”

Section: Discussionmentioning

confidence: 99%

“…However, the naturally lower P50 in 100mm Hg indicate that the given levels of saturation would not be achieved in the normal lung breathing air. Data calculated from Teisseire et al (1985).…”

Section: Discussionmentioning

confidence: 99%

The influence of haemoglobin affinity for oxygen on tumour radiosensitivity

Hirst¹,

Wood²

1987

Br J Cancer

View full text Add to dashboard Cite

Summary Appropriate control of the affinity of haemoglobin for oxygen is fundamental to the efficient oxygenation of our tissues. Important modifiers of this relationship are pH, CO2 concentration and the intraerythrocytic level of 2, 3-diphosphoglycerate (2,3-DPG). We have studied the influence of haemoglobin affinity on the radiosensitivity of the RIF-1 sarcoma in the mouse. Changes in haemoglobin affinity were induced by exposing donor mice to either 10% oxygen, normal air, or 100% oxygen for 48 h. Blood was drawn from these animals and exchanged transfused into tumour-bearing mice immediately before irradiation. Transfusion of blood from mice breathing 10% oxygen carried a lowered haemoglobin affinity and produced marked radiosensitization of the tumours in the recipients; transfusion with normal blood had no significant effect and transfusions from mice breathing 100% oxygen caused a small increase in radioresistance. Measurements of the level of 2,3-DPG in the blood of these groups showed higher concentrations in the oxygen-deprived animals than in controls but no significant change in animals exposed to 100% oxygen. These results demonstrate that alterations in haemoglobin affinity, probably resulting from changes in 2,3-DPG levels, can have a powerful influence on tumour radiosensitivity. We feel that this mechanism could have considerable clinical importance.The control of oxygen transport by haemoglobin is mediated through allosteric modification of its molecular structure. The best known process involves CO2 which, in binding to haemoglobin to form carbaminohaemoglobin, reduces haemoglobin affinity for oxygen. In addition, CO2 in solution forms bicarbonate which lowers pH and also reduces affinity. These processes are commonly known as the Bohr effect (Bohr et al., 1904) and lead to the advantageous situation in which with every pass through the tissues 02 can be released preferentially whenever the red blood cell encounters regions where CO2 has accumulated and metabolism is rapid. Conversely in the lungs, where CO2 is washed out, haemoglobin affinity is increased facilitating oxygen uptake.More recently the existence of other control systems has been demonstrated (Benesch & Benesch, 1967;Chanutin & Curnish, 1967). The most important of these allosteric modifiers is 2,3-diphosphoglycerate (2,3-DPG) which binds to haemoglobin in a manner which reduces its oxygen affinity. Changes in erythrocyte 2,3-DPG concentration are of fundamental importance in the physiological adaptation to conditions of reduced oxygen availability, such as are encountered at high altitudes or in anaemia (see Thomas et al., 1974, for review (Honig, 1981).The potential importance of this system for the delivery of oxygen to tumours was first recognized by Siemann et al. (1979). In their experiments tumour-bearing mice were exposed to a reduced oxygen atmosphere (12%) for up to 48h before irradiation of the tumours at normal or higher than normal oxygen tensions; this procedure gave tumour sensitization consistent with an approx...

show abstract

“…While theoretical considerations predict the beneficial effect of lowered oxygen affinity (TuREcK et al, 1973;WILLFORD et al, 1982), it has not been unequivocally proved by experimental and clinical studies THOMSON et al, 1974;TEISSEIRE et al, 1979;VEICSTEINAS et al, 1984). A difficulty encountered in experimental approach to this problem is that in vitro or in vivo production of blood with low oxygen affinity is sometimes not so easy (TEISSEIRE et al, 1985). Incubation of red cells with inosine-pyruvate-inorganic phosphate (IPP) mixture (DUHM, 1971; has been most frequently used to stimulate the synthesis of red cell 2,3-diphosphoglycerate (DPG) which, in turn, exerts a potent in situ lowering effect upon the oxygen affinity (BENESCH and BENESCH, 1967;CHANUTIN and CURNISH, 1967).…”

mentioning

confidence: 99%

Incubation procedure effective for decreasing oxygen affinity of red cells in the dog.

et al. 1986

View full text Add to dashboard Cite

Because oxygen affinity of canine red cells had been hardly reduced by the conventional procedure of incubation with inosinepyruvate-phosphate or dihydroxyacetone-pyruvate-phosphate, incubation with acidified phosphoenolpyruvate (PEP) medium was tested for its effect on oxygen affinity. As this procedure had been shown to induce a significant increase of 2,3-diphosphoglycerate (DPG) in human red cells, it was also proved to be effective for lowering oxygen affinity of canine red cells. Incubation with 15 mM PEP in ACD medium (pH 6.0) at 37°C for 30 min was the standard procedure established after critical scrutiny. The incubated blood exhibited an average increase in half-saturation pressure (P50) amounting to 7 Torr (pH 7.40, P~02 40 Torr, 37°C), with no appreciable hemolysis and little change in mean corpuscular hemoglobin concentration. Results with Dowex 1-X8 anion exchange chromatography of acid soluble phosphates from the incubated red cells suggested that PEP penetrated rapidly through, the canine red cell membrane and was metabolized to DPG via the retrograde process of the Embden-Meyerhof glycolytic pathway.

show abstract

Physiological effects of high-P50 erythrocyte transfusion on piglets

Cited by 49 publications

References 0 publications

Modulation of Perfusion and Oxygenation by Red Blood Cell Oxygen Affinity during Acute Anemia

Modulation of Perfusion and Oxygenation by Red Blood Cell Oxygen Affinity during Acute Anemia

The influence of haemoglobin affinity for oxygen on tumour radiosensitivity

Incubation procedure effective for decreasing oxygen affinity of red cells in the dog.

Contact Info

Product

Resources

About