Physiological Effects of Salmon Milt Nucleoprotein on Movement, Stress Tolerance and Lifespan of <i>C. elegans</i>

Abstract

In recent years, various physiological functions of salmon milt extract, which consists of nucleic acid and nucleoprotein, have been reported. The objective of this study is to analyze the physiological function and its mechanism of salmon milt extract (NG) on nematodes (C. elegans). The wild type nematode N2 strain was bred on the plate containing of NG for four days, and its body length increased depending on NG concentration. When nematodes were bred with NG for a longer period, average lifespan was increased, and survival rate was increased by up to 20%. Generally, the movement of nematodes decreases with longer breeding period (i.e. aging). Analysis of movement (both gross thrashing movement and local pumping movement) showed that NG suppressed this decrease f movement with aging. Furthermore, the deease of survival rate by heat stress and oxidative stress was suppressed by NG administration. Nile Red staining analysis showed that fat accumulation varied depending on the concentration of NG. RT-PCR analysis revealed that the mRNA expression levels of the stress resistance genes sod-3 and sod-4 were increased. These results indicated that NG administration increased the expression of stress-tolerance-related genes, promoted stress tolerance, increased movement and prolonged lifespan in nematode

Killed Bifidobacterium longum enhanced stress tolerance and prolonged life span of Caenorhabditis elegans via DAF-16

“…Furthermore, its genome and cell lineages are well described. Lately, it has been used to evaluate the effect of certain food and their related functional components, including the anti-ageing effect of nucleoprotein extracted from salmon milt ( 2 ) , longevity and stress tolerance effects of catalpol extracted from rehmannia roots ( 3 ) , longevity effect of oreanolic acid ( 4 ) and others by feeding them along with E. coli to C. elegans .…”

“…Longevity genes such as SIR2 and TOR1 of budding yeast ( 5 , 6 ) ; sir-2.1, daf-16 and age-1 of C. elegans ( 7 , 8 ) ; and Sir2 and dFOXO of Drosophila ( 9 ) are well described. These genes mediate longevity ( 10 – 12 ) , lower the reactive oxygen species (ROS) level ( 3 , 13 ) , suppress ageing ( 2 ) and increase stress tolerance ( 3 , 4 ) . For example, activated DAF-16 translocates into the nucleus and acts as a transcription factor ( 14 ) for genes related to stress tolerance, such as sod-3 ( 15 , 16 ) and hsp-12.6 ( 17 ) .…”