Hemp sesbania (Sesbania exaltata) plants (>30 cm tall) sprayed with hot water (45˚C-95˚C), followed by spray applications of fungal spores of Colletotrichum truncatum (CT) at 1.0 × 10 7 spores/ml −1 and 22˚C-25˚C, suspended either in: 0.2% Silwet L-77 surfactant (SW); unrefined corn oil (CO)/distilled water (1:1, v:v); or 0.2% SW in CO were controlled by 80%-95%, 12 days after treatment (DAT) under greenhouse conditions. These treatments also reduced dry weight accumulation of this weed. Plants treated with hot water without CT were also injured at temperatures ≥35˚C (5% mortality), and 60% mortality at 95˚C. Artificial dew treatments (25˚C, 12 h), imposed on plants after the treatment protocols above, had little or no effect on weed mortality or dry weight reduction compared to treated plants without dew. Under field conditions, 85% control of hemp sesbania was achieved 12-15 DAT when a pre-treatment with hot water (65˚C) was followed immediately with a CT application at the spore concentration as described above. Plants in field tests treated with CT without a hot water treatment were visually unaffected, with no mortality or plant biomass reductions recorded 15 DAT. These results suggest that use of hot water may be an important tool for improving the infectivity and bioherbicidal potential of some plant pathogens.