Physiological evaluation of the responses of Larix olgensis families to drought stress and proteomic analysis of the superior family

Abstract

ABSTRACT. The conifer Larix olgensis has been analyzed to delineate physiological and proteomic changes that occur under drought stress. Studies of the deleterious effects of drought in the larch families have mainly focused on photosynthesis. In the present study, when the intensity of drought was increased, plant height was inhibited as both POD and MDA levels increased, which indicates oxidative stress. Two-dimensional gel electrophoresis analysis detected 23 significantly differentially expressed proteins, of which 18 were analyzed by peptide mass fingerprinting by using MALDI-TOF/TOF. Eight spots were found to be up-regulated, while the other 10 spots were down-regulated during drought stress. The proteins that were induced by drought treatment have been implicated in the physiological changes that occurred. These results could provide additional information that could lead to a better understanding of the molecular basis of drought-sensitivity in larch plants.

Proteomics, Holm Oak (Quercus ilex L.) and Other Recalcitrant and Orphan Forest Tree Species: How do They See Each Other?

“…Responses to biotic and abiotic stresses are considered as the most covered topic in plant research, in general, and forest tree research, in particular. For instance, nutritional deficiency studies have been approached using proteomics in Fagus sylvatica and P. massoniana [72,73], oxidative stress in Populus simonii x P. nigra [74], salt in Robinia pseudoacacia and Paulownia fortune [75,76], drought in Platycladus orientalis [77], P. halepensis and Larix olgensis [78,79], UV light in P. cathayana , and P. radiata [80,81,82], heavy metals in P. yunnanensis [83], and pathogens in P. tomentosa [84]. Quercus ilex responses to abiotic (drought) and biotic ( P. cinnamomi) stresses and the variability in such response among populations are a key objective of our research, ultimately aimed at characterizing and selecting elite genotypes with high levels of tolerance and resistance to both stresses, conferring fitness advantages in a climate change scenario.…”

Glutathione, carbohydrate and other metabolites of Larix olgensis A. Henry reponse to polyethylene glycol-simulated drought stress

“…Molecular responses to drought stress have been extensively studied in broad-leaved species, but studies on coniferous species are limited [ 2 ]. Larix olgensis A. Henry is a more drought-tolerant conifer [ 3 ], which is mainly distributed in the Changbai Mountain and Laoyeling Mountain area of Northeast China. The lack of research on basic drought stress mechanisms in L .…”

Metabolic Response of Larix Olgensis A. Henry to Polyethylene Glycol-simulated Drought Stress

“…However, in plants, sucrose also acts as a signal substance, regulates transporters, and induces the expression of resistance genes. In addition, sucrose functions as an antioxidant [3]. Sucrose synthase is an extremely important soluble enzyme in sucrose metabolism, which is found in the cytoplasm.…”

“…Molecular responses to drought stress have been extensively studied in broad-leaved species, but studies on coniferous species are limited [2]. Larix olgensis A. Henry is a more drought-tolerant conifer [3], which is mainly distributed in the Changbai Mountain and Laoyeling Mountain area of Northeast China. The lack of research on basic drought stress mechanisms in L. olgensis has hindered the improvement of larch yield and wood quality in arid environments to some extent.…”