Physiological features of rapeseed plants expressing the gene for an antimicrobial peptide cecropin P1

Захарченко, Н. С.; Buryanov, Yа. I.; Лебедева, А. А.; Pigoleva, S. V.; Ветошкина, Д. В.; Loktyushov, E. V.; Chepurnova, M. A.; Kreslavski, Vladimir D.; Kosobryukhov, A. A.

doi:10.1134/s1021443713030163

Cited by 15 publications

(8 citation statements)

References 23 publications

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“…Also, transgenic potato with the AMP gene msrA3 showed increased resistance to pathogens Fusarium solani and abiotic stress-induced by darkness, wounding, and high temperature (Goyal et al 2013). It was shown previously that expression of AMP cecropin P1 increased the resistance of transgenic rape plant to pathogenic E. carotovora and F. sporotrichioides though contributed to some decrease in the rate of photosynthesis under infection (Zakharchenko et al 2013).…”

Section: Discussionmentioning

confidence: 89%

“…Previously, we obtained transgenic oilseed rape plants with the gene of the antimicrobial peptide cecropin P1 and carried out molecular genetic analysis of the plants received (Zakharchenko et al 2013). In this paper, we conducted a physiological and biochemical analysis of transgenic plants and studied their safety for beneficial associative bacteria.…”

Section: Resultsmentioning

confidence: 99%

“…Earlier, we reported the production of transgenic plants expressing antimicrobial peptide cecropin P1 gene that resulted in increased resistance against certain pathogens and stress factors (Zakharchenko et al 2013). Also, we successfully demonstrated diverse positive effects of tomato, sugar beet, white cabbage plants colonization with different symbiotic bacteria (Pigoleva et al 2009(Pigoleva et al , 2020.…”

Section: Introductionmentioning

confidence: 85%

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The Brassica napus L. plants expressing antimicrobial peptide cecropin P1 are safe for colonization by beneficial associative microorganisms

Захарченко

Furs

Рукавцова

et al. 2021

Journal of Plant Interactions

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This work was aimed to study the opportunity of transgenic oilseed rape (Brassica napus L.), expressing the gene of antimicrobial peptide cecropin P1 (cecP1) to be inhabited with associative microorganisms Methylobacterium mesophilicum and Pseudomonas aureofaciens. Previously, resistance was demonstrated to the microbial pathogens:Erwinia carotovora B15, Pseudomonas syringae, Fusarium oxysporum, and Botrytis cinerea. Analysis of the fatty acid composition of transgenic plant seeds showed an increase in the proportion of unsaturated fatty acids. Plants studied showed increased photosynthetic activity in oxidative stress induced by paraquat instead of in oxidative stress and UV radiation compared to control ones. A study of the interaction of transgenic plants with the associative microorganisms showed that bacteria were located in all initially colonized plants, as well as in plants obtained after several passages of microproliferation. Accordingly, transgenic plants, containing the cecP1 gene were actively colonized by associative bacteria, indicating their safety for associative bacteria, indicating their safety for beneficial microorganisms.

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Section: Discussionmentioning

confidence: 89%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 85%

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The Brassica napus L. plants expressing antimicrobial peptide cecropin P1 are safe for colonization by beneficial associative microorganisms

Захарченко

Furs

Рукавцова

et al. 2021

Journal of Plant Interactions

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“…6d). The enhanced antioxidant defense inherent in the CP1 plants was observed not only in the experiments with the plants infected with phytopathogenes, but also in the case of the nonin fected CP1 plants exposed the impact of both herbi cide paraquat and UV light [31]. It is likely that enhanced expression of transgene CP1 in plants can significantly enhance both their antimicrobial and antioxidant defense.…”

Section: Discussionmentioning

confidence: 95%

Novel expression system for enhanced synthesis of antimicrobial peptide cecropin P1 in plants

Захарченко

Strizhov

Shkolnaya

et al. 2015

Russ J Plant Physiol

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A gene of antimicrobial peptide cecropin P1 (CP1) was inserted into the vector plasmid pPCV91 under the control of promoter 35S RNA of cauliflower mosaic virus (CaMV 35S) containing four enhancer sequences CaMV 35S and nontranslated leader sequence Ω RNA of tobacco mosaic virus. The recombinant vector obtained was used for agrobacterial transformation of tobacco plants (Nicotiana tabacum L., variety Sam sun) with the polymerase chain reaction (PCR) based method. The presence of gene CP1 in the genome of plants was proven by western blot analysis and testing the antibiotic activity of plant extracts. In different plant lines, the level of cecropin P1 synthesis amounted 0.02-0.2% of total soluble plant leaf protein. The transgenic plants, unlike the control ones, displayed enhanced tolerance to phytopathogenic microorganisms and oxidative stress. It was established that the ability of the transgenic plants to express cecropin P1 is transmitted to progeny.Abbreviations: SOD-superoxide dismutase; Cf-cefatoxime; CaMV 35S-promoter 35S RNA cauliflower mosaic virus (CaMV); CP1-cecropin P1; Hm-hygromycin; hpt-hygro mycin phosphotransferase gene; pnos-promoter of agrobacterial nopalin synthase.

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“…In order to achieve a better characterization of the interesting peptide (e.g., to determine action mechanisms, structure and function, or its potential use as a medicine) it is necessary to obtain a reasonable amount of this peptide; however, there are some issues that prevent it from being obtained in a massive way, such as the low yield of the interest peptide when it is purified directly from the natural host, the production of potential virulence factors of some bacterial producers and the high production costs by chemical synthesis (Parachin et al, 2012;Arbulu et al, 2015). Due to the aforementioned, the production of antimicrobial peptides by recombinant pathways using heterologous expression systems such as bacteria (Mesa-Pereira et al, 2017), yeast (Arbulu et al, 2015), microalgae (Mu et al, 2012) and plants (Zakharchenko et al, 2013) has become a rapidly expanding research area.…”

Section: Discussionmentioning

confidence: 99%

Heterologous expression of bacteriocin E-760 in Chlamydomonas reinhardtii and functional analysis

Quezada-Rivera

Soria-Guerra

Pérez-Juárez

et al. 2019

Phyton

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The use of antimicrobial peptides (AMPs) synthesized by bacteria (bacteriocins) is an alternative for combating multidrug resistant bacterial strains and their production by recombinant route is a viable option for their mass production. The bacteriocin E-760 isolated from the genus Enterococcus sp. has been shown to possess inhibitory activity against Gram-negative and Gram-positive bacteria. In this study, the expression of a chimeric protein coding for E-760 in the nucleus of C. reinhardtii was evaluated, as well as, its antibacterial activity. The synthetic gene E-760S was inserted into the genome of C. reinhardtii using Agrobacterium tumefaciens. A transgenic line was identified in TAP medium with hygromycin and also by PCR. The increment in the culture medium temperature of the transgenic strain at 35 °C for 10 minutes, increased the production level of the recombinant protein from 0.14 (Noninduced culture, NIC) to 0.36% (Induced culture, IC) of total soluble proteins (TSP); this was quantified by an ELISA assay. Recombinant E-760 possesses activity against Staphylococcus aureus in 0.34 U log, Streptococcus agalactiae in 0.48 U log, Enterococcus faecium in 0.36 U log, Pseudomonas aeruginosa in 2 U log and for Klebsiella pneumoniae, the activity was 0.07 U log. These results demonstrate that the nucleus transformation of C. reinhardtii can function as a stable expression platform for the production of the synthetic gene E-760 and it can potentially be used as an antibacterial agent.

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Physiological features of rapeseed plants expressing the gene for an antimicrobial peptide cecropin P1

Cited by 15 publications

References 23 publications

The Brassica napus L. plants expressing antimicrobial peptide cecropin P1 are safe for colonization by beneficial associative microorganisms

The Brassica napus L. plants expressing antimicrobial peptide cecropin P1 are safe for colonization by beneficial associative microorganisms

Novel expression system for enhanced synthesis of antimicrobial peptide cecropin P1 in plants

Heterologous expression of bacteriocin E-760 in Chlamydomonas reinhardtii and functional analysis

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