Physiological functions at single-cell level of Lactobacillus spp. isolated from traditionally fermented cabbage in response to different pH conditions

Olszewska, Magdalena A.; Kocot, Aleksandra Maria; Łaniewska-Trokenheim, Łucja

doi:10.1016/j.jbiotec.2015.02.031

Cited by 9 publications

(6 citation statements)

References 40 publications

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“…Similar results were found in Lactococcus lactis during pH-controlled fermentation [16] and Lactobacillus spp. when pH changed [17]. At the same time, we detected that the survival rate of the total population (FCM-T) was between the FCM-V and FCM-I subpopulations, which confirmed that the acid resistance of the total population was a comprehensive reflection of different subpopulations.…”

Section: Discussionsupporting

confidence: 61%

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bglG Regulates the Heterogeneity Driven by the Acid Tolerance Response in Lacticaseibacillus paracasei L9

Shen,

Lin,

Zhai

et al. 2023

Foods

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The acid tolerance of lactic acid bacteria is crucial for their fermentation and probiotic functions. Acid adaption significantly enhances the acid tolerance of strains, and the phenotypic heterogeneity driven by the acid tolerance response (ATR) contributes to this process by providing a selective advantage in harsh environments. The mechanism of heterogeneity under the ATR is not yet clear, but individual gene expression differences are recognized as the cause. In this study, we observed four heterogeneous subpopulations (viable, injured, dead, and unstained) of Lacticaseibacillus paracasei L9 (L9) induced by acid adaption (pH 5.0, 40 min) using flow cytometry. The viable subpopulation represented a significantly superior acid tolerance to the injured subpopulation or total population. Different subpopulations were sorted and transcriptomic analysis was performed. Five genes were found to be upregulated in the viable subpopulation and downregulated in the injured subpopulation, and bglG (LPL9_RS14735) was identified as having a key role in this process. Using salicin (glucoside)-inducing gene expression and gene insertion mutagenesis, we verified that bglG regulated the heterogeneity of the acid stress response and that the relevant mechanisms might be related to activating hsp20. This study provides new evidence for the mechanism of the ATR and may contribute to the theoretical basis of improving the acid tolerance of Lacticaseibacillus paracasei L9.

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Section: Discussionsupporting

confidence: 61%

“…The heterogeneity found in Lacticaseibacillus casei [15], Lactococcus lactis [16], and Lactobacillus spp. [17] when exposed to the acid stress response may be explained as follows: (i) The distribution of subpopulations varies with Ph. (ii) There are significant differences in the culturability and acid tolerance between subpopulations.…”

Section: Introductionmentioning

confidence: 99%

bglG Regulates the Heterogeneity Driven by the Acid Tolerance Response in Lacticaseibacillus paracasei L9

Shen,

Lin,

Zhai

et al. 2023

Foods

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“…Due to their small size and their potential mobility during imaging experimentations, bacteria do not fit perfectly with specific organelle monitoring. Furthermore, diacetate derivatives (FDA and CFDA 5) are perfect candidates for monitoring bacterial enzymatic activity, explaining why recent studies are mainly using fluorescein at its purpose [89][90][91][92][93]. However, some work also used these fluorescein derivatives for their pH-sensitivity.…”

Section: Bacterial Growthmentioning

confidence: 99%

Fluorescein Derivatives as Fluorescent Probes for pH Monitoring along Recent Biological Applications

Guern

Mussard

Gaucher

et al. 2020

IJMS

112

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Potential of hydrogen (pH) is one of the most relevant parameters characterizing aqueous solutions. In biology, pH is intrinsically linked to cellular life since all metabolic pathways are implicated into ionic flows. In that way, determination of local pH offers a unique and major opportunity to increase our understanding of biological systems. Whereas the most common technique to obtain these data in analytical chemistry is to directly measure potential between two electrodes, in biological systems, this information has to be recovered in-situ without any physical interaction. Based on their non-invasive optical properties, fluorescent pH-sensitive probe are pertinent tools to develop. One of the most notorious pH-sensitive probes is fluorescein. In addition to excellent photophysical properties, this fluorophore presents a pH-sensitivity around neutral and physiologic domains. This review intends to shed new light on the recent use of fluorescein as pH-sensitive probes for biological applications, including targeted probes for specific imaging, flexible monitoring of bacterial growth, and biomedical applications.

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“…1 i 2). Przydatność metody FISH do identyfikacji bakterii fermentacji mlekowej z fermentowanych surowców pochodzenia roślinnego opisano we wcześniejszych badaniach [19]. Po zastosowaniu tych samych sond również zidentyfikowano dwa typowe dla surowców roślinnych gatunki, L. plantarum i L. brevis.…”

Section: Wyniki I Dyskusjaunclassified

Fluorescencyjna hybrydyzacja in situ w identyfikacji i ocenie aktywności fizjologicznej Lactobacillus spp. w soku z jagód goji

Kocot¹,

Mruk²,

Olszewska³

2017

zntj

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W związku z popularyzacją trendu „Super Food” konsumenci oczekują obecności na rynku produktów o szerokim spektrum oddziaływania na organizm człowieka. Sok z jagód goji charakteryzuje się wieloma dobroczynnymi właściwościami, a wzbogacenie go w potencjalnie probiotyczne kultury Lactobacillus spp. może dodatkowo zwiększyć jego wartość biologiczną pod warunkiem zachowania przez szczepy żywotności. Celem pracy była identyfikacja i ocena aktywności fizjologicznej komórek Lactobacillus spp. w soku z jagód goji z zastosowaniem metody fluorescencyjnej hybrydyzacji in situ (FISH). W celu identyfikacji Lactobacillus spp. przeprowadzono hybrydyzację z sondami oligonukleotydowymi rRNA, w tym z sondą dla domeny Eubacteria (Eub338), nonsensowną (Non338), a także dla grupy bakterii fermentacji mlekowej (Lab158) i 11 sond specyficznych dla różnych gatunków z rodzaju Lactobacillus. Analizę aktywności fizjologicznej komórek Lactobacillus spp. w soku z jagód goji przeprowadzono sondą Eub338 bezpośrednio po zaszczepieniu oraz 3., 8., 15., 22. i 29. dnia przechowywania w temp. 20 ºC (± 2 ºC) oraz 6 ºC i porównano z wynikami metody płytkowej. Metoda FISH pozwoliła na określenie przynależności gatunkowej 8 z 9 badanych szczepów, które zaklasyfikowano do L. plantarum oraz L. brevis. Wykazano ponadto, że w wyniku przechowywania soku z jagód goji w temp. 20 ºC (± 2 ºC) aktywność fizjologiczna komórek podlegała znacznym zmianom, czyli zmniejszyła się w ostatnim dniu przechowywania i po porównaniu z wynikami metody płytkowej można wskazać na stan niehodowalności komórek. Natomiast po przechowywaniu w temp. 6 ºC aktywność fizjologiczna była stabilna, gdyż liczba komórek utrzymywała się na wysokim poziomie do ostatniego dnia przechowywania. Badania przeprowadzone w tym zakresie wskazują na przydatność metody FISH w detekcji i poznawaniu fizjologii pałeczek Lactobacillus spp.

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Physiological functions at single-cell level of Lactobacillus spp. isolated from traditionally fermented cabbage in response to different pH conditions

Cited by 9 publications

References 40 publications

bglG Regulates the Heterogeneity Driven by the Acid Tolerance Response in Lacticaseibacillus paracasei L9

bglG Regulates the Heterogeneity Driven by the Acid Tolerance Response in Lacticaseibacillus paracasei L9

Fluorescein Derivatives as Fluorescent Probes for pH Monitoring along Recent Biological Applications

Fluorescencyjna hybrydyzacja in situ w identyfikacji i ocenie aktywności fizjologicznej Lactobacillus spp. w soku z jagód goji

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