Physiological, genomic and transcriptional diversity in responses to boron deficiency in rapeseed genotypes

Hua, Yingpeng; Zhou, Ting; Ding, Guangda; Yang, Qingyong; Shi, Lei; Xu, Fangsen

doi:10.1093/jxb/erw342

Cited by 40 publications

(35 citation statements)

References 61 publications

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“…The development of molecular markers associated with the QTLs should help breeders to identify efficient genotypes. However, substantial research remains to be done to transfer the knowledge on the physiology and genetics of boron utilization efficiency into boron-efficient plant varieties with good growth, quality, and yield [201,[203][204][205][206].…”

Section: Boron Deficiencymentioning

confidence: 99%

Boron Toxicity and Deficiency in Agricultural Plants

Brdar-Jokanović

2020

IJMS

281

119

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Boron is an essential plant micronutrient taken up via the roots mostly in the form of boric acid. Its important role in plant metabolism involves the stabilization of molecules with cis-diol groups. The element is involved in the cell wall and membrane structure and functioning; therefore, it participates in numerous ion, metabolite, and hormone transport reactions. Boron has an extremely narrow range between deficiency and toxicity, and inadequate boron supply exhibits a detrimental effect on the yield of agricultural plants. The deficiency problem can be solved by fertilization, whereas soil boron toxicity can be ameliorated using various procedures; however, these approaches are costly and time-consuming, and they often show temporary effects. Plant species, as well as the genotypes within the species, dramatically differ in terms of boron requirements; thus, the available soil boron which is deficient for one crop may exhibit toxic effects on another. The widely documented intraspecies genetic variability regarding boron utilization efficiency and toxicity tolerance, together with the knowledge of the physiology and genetics of boron, should result in the development of efficient and tolerant varieties that may represent a long-term sustainable solution for the problem of inadequate or excess boron supply.

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Section: Boron Deficiencymentioning

confidence: 99%

Boron Toxicity and Deficiency in Agricultural Plants

Brdar-Jokanović

2020

IJMS

281

119

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“…Based on sequence and expression analyses, BnaA03.NIP5;1b was subsequently suggested to be the responsible gene in the B efficiency loci of cv. Q10 (Xu et al ., , ; Zhao et al ., , ; Zhang et al ., ,b; Hua et al ., ,b). However, neither BnaA03.NIP5;1b nor BnaC02.NIP5;1a have been tested for their B permeability, although it is known that even highly homologous NIPs can possess different substrate selectivities (Zhao et al ., ; Mitani‐Ueno et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Boron demanding tissues of Brassica napus express specific sets of functional Nodulin26‐like Intrinsic Proteins and BOR1 transporters

et al. 2019

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SummaryThe sophisticated uptake and translocation regulation of the essential element boron (B) in plants is ensured by two transmembrane transporter families: the Nodulin26‐like Intrinsic Protein (NIP) and BOR transporter family. Though the agriculturally important crop Brassica napus is highly sensitive to B deficiency, and NIPs and BORs have been suggested to be responsible for B efficiency in this species, functional information of these transporter subfamilies is extremely rare. Here, we molecularly characterized the NIP and BOR1 transporter family in the European winter‐type cv. Darmor‐PBY018. Our transport assays in the heterologous oocyte and yeast expression systems as well as in growth complementation assays in planta demonstrated B transport activity of NIP5, NIP6, NIP7 and BOR1 isoforms. Moreover, we provided functional and quantitative evidence that also members of the NIP2, NIP3 and NIP4 groups facilitate the transport of B. A detailed B‐ and tissue‐dependent B‐transporter expression map was generated by quantitative polymerase chain reaction. We showed that NIP5 isoforms are highly upregulated under B‐deficient conditions in roots, but also in shoot tissues. Moreover, we detected transcripts of several B‐permeable NIPs from various groups in floral tissues that contribute to the B distribution within the highly B deficiency‐sensitive flowers.

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“…B. napus (A n A n C n C n , 2n = 4x = 38) is an important oil crop species in the world, which most likely originated in domestication from the natural spontaneous hybridization between the diploid ancestors B. oleracea (C o C o , 2n = 2x = 18) and B. rapa (A r A r , 2n = 2x = 20) ~7,500 years ago, resulting in chromosome doubling (Chalhoub et al, 2014 ). In B. napus , we have previously identified six orthologous genes of NIP5;1 , with BnaA03.NIP5;1b , and BnaC02.NIP5;1a proposed as candidate genes underlying the major-effect B efficiency QTLs qBEC-A3a and qBEC-C2a , respectively (Hua et al, 2016a , b ). AtNIP6;1 is essential for preferential distribution of B into growing tissues under low B (Tanaka et al, 2008 ).…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Characterization of the Aquaporin Gene Family and Transcriptional Responses to Boron Deficiency in Brassica napus

Yuan

Hua

et al. 2017

Front. Plant Sci.

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Aquaporins (AQPs) are an abundant protein family and play important roles to facilitate small neutral molecule transport across membranes. Oilseed rape (Brassica napus L.) is an important oil crop in China and elsewhere in the world, and is very sensitive to low boron (B) stress. Several AQP family genes have been reported to be involved in B transport across plasma membranes in plants. In this study, a total of 121 full-length AQPs were identified and characterized in B. napus (AC genome), and could be classified into four sub-families, including 43 PIPs (plasma membrane intrinsic proteins), 35 TIPs (tonoplast intrinsic proteins), 32 NIPs (NOD26-like intrinsic proteins), and 11 SIPs (small basic intrinsic proteins). The gene characteristics of BnaAQPs were similar to those of BraAQPs (A genome) and BolAQPs (C genome) including the composition of each sub-family, gene structure, and substrate selectivity filters. The BnaNIP was the most complex AQP sub-family, reflecting the composition of substrate selectivity filter structures which affect the permeation of solution molecules. In this study, the seedlings of both B-efficient (QY10) and B-inefficient (W10) cultivars were treated with two boron (B) levels: deficient (0.25 μM B) and sufficient (25 μM B). The transcription of AQP genes in root (R), juvenile leaf (JL), and old leaf (OL) tissues of both cultivars was investigated under B deficient and sufficient conditions. Transcription of most BnaPIPs and BnaTIPs was significantly increased compared with other BnaAQPs in all the three tissues, especially in the roots, of both B-efficient and B-inefficient cultivars under both B conditions. With B deprivation, the expression of the majority of the BnaPIPs and BnaTIPs was down-regulated in the roots. However, the BnaNIPs were up-regulated. In addition, the BnaCnn_random.PIP1;4b, BnaPIP2;4s, BnaC04.TIP4;1a, BnaAnn_random.TIP1;1b, and BnaNIP5;1s (except for BnaA07.NIP5;1c and BnaC06.NIP5;1c) exhibited obvious differences at low B between B-efficient and B-inefficient cultivars. These results will help us to understand boron homeostasis in B. napus.

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Physiological, genomic and transcriptional diversity in responses to boron deficiency in rapeseed genotypes

Cited by 40 publications

References 61 publications

Boron Toxicity and Deficiency in Agricultural Plants

Boron Toxicity and Deficiency in Agricultural Plants

Boron demanding tissues of Brassica napus express specific sets of functional Nodulin26‐like Intrinsic Proteins and BOR1 transporters

Genome-Wide Identification and Characterization of the Aquaporin Gene Family and Transcriptional Responses to Boron Deficiency in Brassica napus

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