Physiological, genomic and transcriptomic comparison of two Brassica napus cultivars with contrasting cadmium tolerance

Wang, Shufeng; Sun, Juan; Li, Shengting; Lü, Kun; Meng, Hongjun; Xiao, Zhongchun; Zhang, Zhen; Li, Jiana; Luo, Feng; Li, Nannan

doi:10.1007/s11104-019-04083-0

Cited by 17 publications

(8 citation statements)

References 41 publications

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“…SOD, POD, APX, and CAT are major enzyme scavengers in plants ( Racchi, 2013 ). SOD enzymes are critical enzymes in the antioxidant system of plants, and scavenge superoxide anions, reacting to produce H 2 O 2 and O 2 , and CAT and APX catalyze the subsequently produced H 2 O 2 to produce water and divalent oxygen ( Wang et al, 2019 ). POD catalyzes substrate oxidation using H 2 O 2 as an electron acceptor, and it is considered to be one of the two major indicators of plant stress tolerance along with SOD ( Shu et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Plant growth regulators mitigate oxidative damage to rice seedling roots by NaCl stress

Wang,

Zhao,

Feng

et al. 2024

PeerJ

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The aim of this experiment was to investigate the effects of exogenous sprays of 5-aminolevulinic acid (5-ALA) and 2-Diethylaminoethyl hexanoate (DTA-6) on the growth and salt tolerance of rice (Oryza sativa L.) seedlings. This study was conducted in a solar greenhouse at Guangdong Ocean University, where ‘Huanghuazhan’ was selected as the test material, and 40 mg/L 5-ALA and 30 mg/L DTA-6 were applied as foliar sprays at the three-leaf-one-heart stage of rice, followed by treatment with 0.3% NaCl (W/W) 24 h later. A total of six treatments were set up as follows: (1) CK: control, (2) A: 40 mg⋅ L−1 5-ALA, (3) D: 30 mg⋅ L−1 DTA-6, (4) S: 0.3% NaCl, (5) AS: 40 mg⋅ L−1 5-ALA + 0.3% NaCl, and (6) DS: 30 mg⋅ L−1 DTA-6+0.3% NaCl. Samples were taken at 1, 4, 7, 10, and 13 d after NaCl treatment to determine the morphology and physiological and biochemical indices of rice roots. The results showed that NaCl stress significantly inhibited rice growth; disrupted the antioxidant system; increased the rates of malondialdehyde, hydrogen peroxide, and superoxide anion production; and affected the content of related hormones. Malondialdehyde content, hydrogen peroxide content, and superoxide anion production rate significantly increased from 12.57% to 21.82%, 18.12% to 63.10%, and 7.17% to 56.20%, respectively, in the S treatment group compared to the CK group. Under salt stress, foliar sprays of both 5-ALA and DTA-6 increased antioxidant enzyme activities and osmoregulatory substance content; expanded non-enzymatic antioxidant AsA and GSH content; reduced reactive oxygen species (ROS) accumulation; lowered malondialdehyde content; increased endogenous hormones GA3, JA, IAA, SA, and ZR content; and lowered ABA content in the rice root system. The MDA, H2O2, and O2− contents were reduced from 35.64% to 56.92%, 22.30% to 53.47%, and 7.06% to 20.01%, respectively, in the AS treatment group compared with the S treatment group. In the DS treatment group, the MDA, H2O2, and O2− contents were reduced from 24.60% to 51.09%, 12.14% to 59.05%, and 12.70% to 45.20%. In summary, NaCl stress exerted an inhibitory effect on the rice root system, both foliar sprays of 5-ALA and DTA-6 alleviated damage from NaCl stress on the rice root system, and the effect of 5-ALA was better than that of DTA-6.

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Section: Discussionmentioning

confidence: 99%

Plant growth regulators mitigate oxidative damage to rice seedling roots by NaCl stress

Wang,

Zhao,

Feng

et al. 2024

PeerJ

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“…SOD and POD are two important antioxidant enzymes that remove ROS ( Wang et al, 2019 ). In our study, the activities of SOD and POD in JB231AC were higher than in 62\3, indicating that JB231AC had a pronounced ability to remove ROS-induced toxic substances.…”

Section: Discussionmentioning

confidence: 99%

“…With the advent of next-generation sequencing technologies, many transcriptomic studies on heavy metal stress have focused mainly on rice ( Oono et al, 2016 ), wheat ( Zhou et al, 2019 ), rapeseed ( Zhang et al, 2018a ), Sedum alfredii ( Yang et al, 2017 ), and mustard ( Zhang D. et al, 2021 ), which have revealed the molecular mechanism of transport, accumulation, and detoxification of Cd in different plants. Two rapeseed genotypes were chosen to investigate the Cd translocation mechanism by transcriptomic comparison, and the results showed that BnNramp2;1 and BnNramp4;2 were two main Cd transporters ( Wang et al, 2019 ). Hu et al (2019) compared the transcriptomes of different tissues of Sedum alfredii under Cd stress, and the results showed that ATP-binding cassette (ABC) transporters exhibited significant enrichment and accounted for approximately one-third of total selected differentially expressed genes.…”

Section: Introductionmentioning

confidence: 99%

Physiological and Transcriptomic Comparison of Two Sunflower (Helianthus annuus L.) Cultivars With High/Low Cadmium Accumulation

Zhatova

et al. 2022

Front. Plant Sci.

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The toxic heavy metal cadmium (Cd) is easily absorbed and accumulated in crops and affects human health through the food chains. Sunflower (Helianthus annuus L.) is a globally important oil crop. In this study, two sunflower cultivars 62\3 (high Cd) and JB231AC (low Cd), were chosen to compare physiological and transcriptomic responses at different Cd concentrations (0, 25, 50, and 100 μM). The results showed that JB231AC had better Cd tolerance than 62\3. The contents of H2O2 and MDA (malondialdehyde) in 62\3 were lower than that in JB231AC under Cd stress, but the activities of SOD (superoxide dismutase) and POD (peroxidase) in JB231AC were higher than in 62\3, which indicated that JB231AC had a strong ability to remove reactive oxygen species (ROS)-induced toxic substances. Many deferentially expressed ABC (ATP-binding cassette) and ZIP (Zn-regulated transporter, Iron-regulated transporter-like protein) genes indicated that the two gene families might play important roles in different levels of Cd accumulation in the two cultivars. One up-regulated NRAMP (Natural resistance-associated macrophage protein) gene was identified and had a higher expression level in 62\3. These results provide valuable information to further understand the mechanism of Cd accumulation and provide insights into breeding new low Cd sunflower cultivars.

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“…The malondialdehyde (MDA) content was determined using the same procedure as Wang [ 16 ]. A 0.5 g frozen shoot sample was weighed and extracted, then homogenized with 5 mL of 0.1% TCA and centrifuged at 12,000× g for 20 min.…”

Section: Methodsmentioning

confidence: 99%

“…At 390 nm, the absorbance was determined. The concentration of hydrogen peroxide (H 2 O 2 ) was determined using a standard curve [ 16 ].…”

Section: Methodsmentioning

confidence: 99%

Phosphorus Regulates the Level of Signaling Molecules in Rice to Reduce Cadmium Toxicity

Chen¹,

Y²,

Yang³

et al. 2022

CIMB

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Phosphorus treatment can reduce Cd accumulation and Cd toxicity in rice, but alterations in the internal regulatory network of rice during this process have rarely been reported. We have removed the effect of cadmium phosphate precipitation from the hydroponic system, treated a pair of different Cd-response rice varieties with different levels of phosphorus and cadmium and examined the changes in physiological indicators and regulatory networks. The results demonstrated that phosphorus treatment significantly reduced Cd accumulation in both types of rice, although the antioxidant systems within the two types of rice produced opposite responses. Overall, 3 mM phosphorus treatment to Cd-N decreased the expression of OsIAA17 and OsACO1 by 32% and 37%, respectively, while increasing the expression of OsNR2 by 83%; these three genes regulate the synthesis of auxin, ethylene, and nitric oxide in rice. IAA and NO levels in rice shoots increased by 24% and 96%, respectively, and these changes contribute to Cd detoxification. The cadmium transporter genes OsHMA2, OsIRT1, and OsABCC1 were significantly down-regulated in Cd-N roots after triple phosphorus treatment. These data suggest that phosphorus treatment can reduce Cd accumulation and enhance Cd resistance in rice by affecting the expression of signaling molecules.

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Physiological, genomic and transcriptomic comparison of two Brassica napus cultivars with contrasting cadmium tolerance

Cited by 17 publications

References 41 publications

Plant growth regulators mitigate oxidative damage to rice seedling roots by NaCl stress

Plant growth regulators mitigate oxidative damage to rice seedling roots by NaCl stress

Physiological and Transcriptomic Comparison of Two Sunflower (Helianthus annuus L.) Cultivars With High/Low Cadmium Accumulation

Phosphorus Regulates the Level of Signaling Molecules in Rice to Reduce Cadmium Toxicity

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