Physiological investigations on the effect of olive and rosemary leaves extracts in male rats exposed to thioacetamide

Abstract

Physiologically, it is known that thioacetamide (TAA) toxicity is generally associated with hepatic fibrosis induction, complicated metabolic disorders and health problems. The capability of extracts of olive and rosemary leaves to attenuate the severe physiological disturbances induced by thioacetamide (TAA) intoxication in male rats has been evaluated. Healthy male Wistar rats were used in the present study and were divided randomly into eight groups. Rats of the first group were served as normal control. Rats of the second group were administrated with TAA. Rats of the third, fourth and fifth groups were exposed to TAA plus olive leaves extract, TAA plus rosemary leaves extract and TAA plus olive and rosemary leaves extracts respectively. The sixth, seventh and eighth groups were supplemented with olive leaves extract, rosemary leaves extract, and olive and rosemary leaves extracts respectively. After 12 weeks of experimental treatments, the levels of serum glucose, total protein, albumin and high density lipoprotein cholesterol were significantly decreased, while the levels of triglycerides, cholesterol, low density lipoprotein cholesterol, very low density lipoprotein cholesterol, creatine kinase and lactate dehydrogenase were statistically increased in rats exposed to TAA. Administration of the studied extracts inhibited the hematobiochemical parameters and improved the physiological disturbances induced by TAA intoxication. Additionally, most improvements were noted in rats administrated with rosemary leaves extract followed by olive and rosemary leaves extracts and olive leaves extract. These results suggested that the effect of these extracts might be due to their antioxidant activities against TAA toxicity.

Designing monographs for Rosmarinus officinalis L. and Lavandula angustifolia L.: Two Lebanese species with significant medicinal potentials

“…32 Rosemary's reported pharmacological actions have been linked to its major chemical constituents including rosmarinic acid. 33 In rosemary leaf, total hydroxycinnamic acid derivatives must not be less than (3 g%) expressed as rosmarinic acid. 23 In the current study, results for the assay of Rosemary leaves for total hydroxycinnamic acid derivatives expressed as rosmarinic acid values are demonstrated in (Table 2).…”

“…Their ability to reduce oxidative damage in diabetes has been demonstrated. 33,61,62 R. officinalis (Rosemary) has been examined for its antioxidant potential and is now recognized as one of medicinal plant species with the highest antioxidant activity. 8 Moreover, in folk medicine, Rosemary is one of the plants used to combat DM in Lebanon.…”

“…The phenolic contents of Rosemary have been reported to have antidiabetic potentials. 8,33 Few results, however, were reported regarding the use of REO as an antidiabetic agent. 18 In this study, various doses of the REO, obtained from (R-AP), were studied for their acute effects in alloxan-diabetic animals.…”

Geographical Characterization of Tunisian Olive Tree Leaves (cv. Chemlali) Using HPLC-ESI-TOF and IT/MS Fingerprinting with Hierarchical Cluster Analysis

“…They have been considered for centuries as an important herbal remedy in Mediterranean countries. Their positive effects on human health are multiple: antioxidant [ 10 – 16 ], hypoglycemic [ 17 ], antimicrobial [ 18 ], and anticancerous [ 19 – 22 ] among others. These properties are generally attributed to the presence of a range of compounds, such as secoiridoids, triterpenes, lignans, and flavonoids.…”

Therapeutic Effects of an Ethanolic Olive Leaves Extract or Bone Marrow Mesenchymal Stem Cells against Liver Injury Induced by Gamma Radiation

“…Administration of olive leaves extract reduced the elevation of level plasma AST ,ALT and liver glycogen caused by carbendazim due to preventing the decline of antioxidant defense system and direct free radical scavenging activity [31] . Olive leaves extract improved biochemical alterations induced by liver tocixity due to the main constituent of olive leaves extract (oleuropein) which is thought to be responsible for pharmacological effect [43] .Oleuropein has high antioxidant activity in vitro ,comparable to a hydrosoluble analog of tocopherol (44) In addition, olive leave extract exhibited a hepatoprotective effect against thioacetamide which caused liver cirrhosis Therapeutic effects of an ethanolic olive leaves… 389 [45] . It also decreased cardiac and hepatic injury together with the amelioration in metabolic and oxidative stress parameters in rat model of diet-induced obesity and diabetes.…”