The Zephyr BioHarness ™ was tested to determine the accuracy of heart rate (HR) and respiratory rate (RR) measurements during 2 exercise protocols in conjunction with either a laboratory metabolic cart (Vmax) or a previously validated portable metabolic system (K4b 2 ). In one protocol, HR and RR were measured using the BioHarness and Vmax during a graded exercise up to VȮ 2max (n = 12). In another protocol, HR and RR were measured using the BH and K4b 2 during sustained exercise (30 % and 50 % VȮ 2max for 20 min each) in a hot environment (30°C, 50 % relative humidity) (n = 6). During the graded exercise, HR but not RR, obtained from the BioHarness was higher compared to the Vmax at baseline and 30 % VȮ 2max (p < 0.05), but showed no significant difference at other stages with high correlation coefficients for both HR (r = 0.87-0.96) and RR (r = 0.90-0.99 above 30 % VȮ 2max ). During the exercise in the heat, there were no significant differences between the BioHarness and K4b 2 system. Correlation coefficients between the methods were low for HR but moderately to highly correlated (0.49-0.99) for RR. In conclusion, the BioHarness is comparable to Vmax and K4b 2 over a wide range of VȮ 2 during graded exercise and sustained exercise in the heat.