Physiological performance of the intertidal Manila clam (Ruditapes philippinarum) to long-term daily rhythms of air exposure

Yin, Xia; Chen, Peng; Hai, Chen; Jin, Wen; Yan, Xiwu

doi:10.1038/srep41648

Cited by 43 publications

(38 citation statements)

References 60 publications

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“…Generally, clams exhibit elevated oxygen consumption rates at high temperatures and low oxygen consumption rates when low dissolved oxygen levels are low (Kang et al, 2016;Lee et al, 2012). Probably, other physiological process such as temperatureinduced hypoxemia, oxidative damage, and apoptosis in the gills largely determined clam survival (Clark et al, 2013;Portner, 2012;Yin, Chen, Chen, Jin, & Yan, 2017).…”

Section: Discussionmentioning

confidence: 99%

At the tipping point: Differential influences of warming and deoxygenation on the survival, emergence, and respiration of cosmopolitan clams

Kim

Park

Sin

2018

Ecology and Evolution

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Although warming and low dissolved oxygen (DO) levels are co‐occurring significant climatic stressors in the ocean, the combined effects of these stressors on marine benthic animals have not been well established. Here, we tested the effects of elevated temperatures and low dissolved oxygen levels on the survival, emerging behavior from sediment, and the respiration of juvenile cosmopolitan Manila clams (Venerupis philippinarum) by exposing them to two temperatures (20 and 23.5°C) and DO levels (3.5 and 6–7 mg/L). Although within previously described tolerable ranges of temperature and DO, this 3.5°C increase in temperature combined with a 50% decrease in DO had a devastating effect on the survival of clams (85% mortality after 8 days). The mortality of clams under normoxia at 23.5°C appeared to be higher than under the low DO condition at 20°C. On the other hand, more clams emerged from sediment under the low DO condition at 20°C than under any other conditions. Oxygen consumption rates were not significantly affected by different conditions. Our results suggest temperature elevation combined with low oxygen additively increases stress on Manila clams and that warming is at least as stressful as low DO in terms of mortality. However, low DO poses another threat as it may induce emergence from sediment, and, thus increase predation risk. This is the first evidence that a combination of warming and deoxygenation stressors should reduce population survival of clams much more so than changes in a single stressor.

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Section: Discussionmentioning

confidence: 99%

At the tipping point: Differential influences of warming and deoxygenation on the survival, emergence, and respiration of cosmopolitan clams

Kim

Park

Sin

2018

Ecology and Evolution

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“…The spats were subjected to indoor incubation and fed with Tetraselmis as the daily diet. In the laboratory, the clams were reared at a density of 2.5 kg/m 3 for 2 weeks for acclimation and the temperature of rearing seawater (salinity 26‰, pre‐filtered with a sand filter tank) was maintained at 18°C (Yin, Chen, Chen, Jin, & Yan, ). All of the culturing seawater was refreshed daily, and the pH fluctuated from 7.8 to 8.1.…”

Section: Methodsmentioning

confidence: 99%

“…The illumination derived from the dim sunlight transmitted from covering windows with opaque curtains, and the photoperiod followed the natural photoperiod. Given that the temperature of fresh seawater filtered from sand filter tank was just 10–12°C, the fresh seawater was heated up to 18°C prior to use to alleviate possible stress stimulation induced by temperature fluctuations (Lin, Wu, & Huang, ; Yin et al, ). During acclimation, the exponentially grown Tetraselmis (equivalent spherical diameter = 13.2 ± 0.54 μm, n = 100, 2 × 10 6 cells/ml) was utilized to feed clams daily.…”

Section: Methodsmentioning

confidence: 99%

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Evaluation of macroalgal detritus as food source for juvenile Manila clam, Ruditapes philippinarum : Effects on growth, amino acid content and fatty acid composition

et al. 2019

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Sufficient high‐quality microalgae are required for indoor nursery of juvenile Ruditapes philippinarum. However, culturing numerous microalgae to support clam feeding is a heavy burden on many hatcheries. The effects of detritus from the macroalgae Ulva pertusa, Chondrus ocellatus and Undaria pinnatifida on the growth, amino acid content and fatty acid profile of R. philippinarum were assessed as potential substitute diets. The green microalga Tetraselmis cordiformis served as comparative diet. Results revealed that the clams ingesting distinct diets presented no significant differences in growth of soft tissues, but the nutritional component of these clams differed dramatically. The clams fed with Undaria + Tetraselmis had the highest content of essential amino acids and proteins. In addition, the clams fed with single macroalgal diets and mixed macroalgal detritus and Tetraselmis showed significantly higher or statistically equal levels in n‐3/n‐6 ratio and docosahexaenoic acid (DHA)/eicosapentaenoic acid (EPA) ratio with respect to Tetraselmis diets. The relative percentages of EPA and DHA in clams fed with Undaria were 28% and 63% higher than those fed with Tetraselmis, and the arachidonic acid abundances in clams ingesting Undaria + Tetraselmis and Tetraselmis were significantly higher than those in clams ingesting other diets. Together, the diets containing single Undaria or mixed Undaria + Tetraselmis produced Manila clams with nutritional advantages in terms of essential amino acids and polyunsaturated fatty acids. Thus, the detritus of macroalgae, especially Undaria, is an appropriate substitute diet, at least partially, for culture of nutrition‐improved R. philippinarum.

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“…In intertidal areas, as a consequence of air exposure, organisms may face prolonged hypoxia and/or anoxia. Although marine bivalves are among the most hypoxia-tolerant macrofauna (Abele et al, 2009;Gray et al, 2002), the impacts of air exposure on the physiological performance of several bivalves have already been observed (Almeida and Bainy, 2006, Andrade et al, 2018, Chandurvelan et al, 2013, Altieri, 2006Letendre et al 2008, 2011, Yin et al, 2017.…”

Section: Introductionmentioning

confidence: 99%

Are the impacts of carbon nanotubes enhanced in Mytilus galloprovincialis submitted to air exposure?

et al. 2018

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Intertidal species are frequently exposed to environmental changes associated with multiple stressors, which they must either avoid or tolerate by developing physiological and biochemical strategies. Some of the natural environmental changes are related with the tidal cycle which forces organisms to tolerate the differences between an aquatic and an aerial environment. Furthermore, in these environments, organisms are also subjected to pollutants from anthropogenic sources. The present study evaluated the impacts in Mytilus galloprovincialis exposed to multi-walled carbon nanotubes (0.01 mg/L MWCNTs) when continuously submersed or exposed to tides (5 h of low tide, 7 h of high tide) for 14 days. Our results demonstrated that mussels were physiologically and biochemically affected by MWCNTs, especially when exposed to tides. In fact, when only exposed to the carbon nanoparticles or only exposed to tides, the stress induced was not enough to activate mussels' antioxidant defenses which resulted in oxidative damage. However, when mussels were exposed to the combination of tides and MWCNTs increased metabolism was observed, associated with a possible higher production of reactive oxygen species (ROS), leading to a significant increase in the activities of antioxidant enzymes (superoxide dismutase, SOD and glutathione peroxide, GPx) and oxidized glutathione content (GSSG), preventing the occurrence of cellular damage, expressed as no lipid peroxidation (LPO) or protein carbonylation (PC). Therefore, organisms seemed to be able to tolerate MWCNTs and air exposure during tidal regime; however, the combination of both stressors induced higher oxidative stress. These findings indicate that the increasing presence of carbon nanoparticles in marine ecosystems can induce higher toxic impacts in intertidal organisms compared to organisms continuously submerged. Also, our results may indicate that air exposure can act as a cofounding factor on the assessment of different stressors in organisms living in coastal systems.

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Physiological performance of the intertidal Manila clam (Ruditapes philippinarum) to long-term daily rhythms of air exposure

Cited by 43 publications

References 60 publications

At the tipping point: Differential influences of warming and deoxygenation on the survival, emergence, and respiration of cosmopolitan clams

At the tipping point: Differential influences of warming and deoxygenation on the survival, emergence, and respiration of cosmopolitan clams

Evaluation of macroalgal detritus as food source for juvenile Manila clam, Ruditapes philippinarum : Effects on growth, amino acid content and fatty acid composition

Are the impacts of carbon nanotubes enhanced in Mytilus galloprovincialis submitted to air exposure?

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