Physiological response and transcriptome analysis of Prunus mume to early salt stress

Abstract

Soil salinisation is an important factor limiting the cultivation and distribution of Prunus mume. Therefore, identifying the regulation mechanism of salt tolerance of P. mume will promote its cultivation and molecular breeding. In this study, the garden tree species P. mume ‘Meiren’ was investigated, with a soil NaCl content (w/w) of 0.3%. Photosynthetic gas exchange parameters, relative electrical conductivity, malondialdehyde, osmoregulation substances, and antioxidant enzyme activity were measured in the early (3d), middle (10d), and late (30d) stages of stress. Salt treatment was applied for 0 h, 6 h, 24 h, and 72 h; following this, the gene library was constructed from the leaves. The results showed that the CBL-CIPK, mitogen-activated protein kinase, calcium-dependent protein kinase, and abscisic acid signalling pathways were involved in salt stress signal transduction of P. mume. Furthermore, bHLH, WRPK, ERF, and MYB were identified as potential key transcription factor families of salt tolerance in P. mume. Combined with determined physiological responses, the results showed that early salt stress inhibited the activities of RuBisCO, chlorophyll synthase, glutamyl tRNA reductase, divinyl reductase, and magnesium chelatase; resultantly, this led to a reduction in the photosynthetic rate. Also, it was found that P5CS, SS, LEA, and dehydrin regulated the synthesis of proline, soluble sugar, and macromolecular protein to alleviate osmotic stress. POD gene plays an important role in scavenging reactive oxygen species due to its regulation of POD activity. The findings of this research reveal the internal mechanism of the physiological response and provide a foundation for the construction of a genetic regulatory network in response to salt stress in P. mume.

Physiological response and proteomics analysis of Reaumuria soongorica under salt stress

“…Rubisco is a key enzyme in the dark reaction of photosynthesis and plays a central role in carbon fixation 39 . Under salt stress, rubisco protein’s expression in Prunus mume leaves was down-regulated 40 , but its expression in Haloxylon salicornicum leaves was up-regulated 41 . We found that rubisco (RCA) expression was down-regulated in the A vs. C and B vs. C comparison groups, suggesting that down-regulated rubisco expression may lead to reduced light energy utilization in R. soongorica leaves while under salt stress.…”

Physiological Response and Proteomics Analysis of Reaumuria soongorica Under Salt Stress

“…Rubisco is a key enzyme in the dark reaction of photosynthesis and plays a central role in carbon xation 36 . Under salt stress, rubisco protein expression in Prunus mume leaves was down-regulated 37 , but expression of Haloxylon salicornicum was up-regulated in the same leaves 38 . We found that rubisco (RCA) expression was down-regulated in the A vs. C and B vs. C comparison groups, suggesting that down-regulated rubisco expression may lead to reduced light energy utilization in R. soongorica leaves while under salt stress.…”

Molecular Mechanisms of CBL-CIPK Signaling Pathway in Plant Abiotic Stress Tolerance and Hormone Crosstalk

“…Turning to salt tolerance, Wang et al [59] studied the salt tolerance mechanisms in Prunus mume 'Meiren' and unveiled a complex interplay between signaling pathways and transcription factors (TFs) that confer resilience to soil salinization. The research demonstrated that the CBL-CIPK pathway, along with mitogen-activated protein kinase, calcium-dependent protein kinase, and ABA signaling pathways, is integral to P. mume's salt stress response.…”