Physiological Response of In Vitro Cultured MAGNOLIASP. to Nutrient Medium Composition

Abstract

The objective of this study was to assess the regeneration response of in vitro cultured Magnolia × soulangeana ‘Alexandrina’ and Magnolia liliiflora ‘Nigra’ to nutrient medium composition. In the primary culture (initiated from dormant axillary buds) combinations of Murashige and Skoog (MS) basal salts with 6-benzylaminopurine and α-naphthaleneacetic acid were tested. The primary explants of cv. ‘Alexandrina’ expressed higher regeneration rate than cv. ‘Nigra’. For both species, the regen eration was most strongly potentiated at addition of 0.25 mg dm−3 of the cytokinin alone. The auxin exerted undesir–able effects. Several basal salts media were applied in proliferation stage and their physiological effects were evaluated in reference to traditionally used MS. At culturing on Chée & Pool C2d Vitis Medium (VM) that is for the first time introduced to magnolia and on MS, M. liliiflora formed more but less elongated shoots than M. soulangeana. However, on VM, substantial increase (25-30%) of the number of axillary shoots and leaves, shoot length and fresh and dry weights over MS was established for both species. This suggested VM as promising composition of nutrients in multiplication stage. Microshoots obtained on MS, VM, Rugini Olive Medium and DKW Juglans Medium were successfully rooted in vitro and subsequently established ex vitro. The findings expand the information on magnolia response to culture conditions and contribute to elaboration of innovative elements of protocols for establishing tissue cultures with high regeneration capacity.

In vitro Plant Regeneration of Magnolia punduana: An Endemic and Threatened Plant Species

“…1E, G). The current findings were in accordance with the report of Abdelmageed et al 2012, Nakamura et al (1995), Sokolov et al (2014) and Radomir (2012) where axillary buds were used for shoot initiation in different species of Magnolia. MS with 90% explant survivability was found to be the most effective medium for culturing axillary bud.…”

An efficient micropropagation protocol for an endangered ornamental tree species (Magnolia sirindhorniae Noot. & Chalermglin) and assessment of genetic uniformity through DNA markers

“…GA 3 has been used to break dormancy and stimulate shoot elongation in different species of magnolias for a long time [46][47][48] . In the present study, GA 3 was found to be important for bud induction, effectively shortening the time of initiation and inducing stronger buds, which is consistent with previous reports 49,50 . However, some plants cultured in vitro did not undergo any significant growth stimulation with GA 3 51 .…”

Morphological and Biochemical Responses to Gibberellic Acid in Magnolia × ‘Spectrum’ in Vitro

“…It has been reported that obtaining high activity of axillary buds is not easy and depends on many factors, including the composition of basal salts, plant growth regulators and the genotype (Biedermann, 1987;Kamenicka and Lanakova, 2000;Podwyszyńska et al, 2000;Parris et al, 2012;Sokolov et al, 2014). Our previous study showed that growth and development of M. × soulangiana 'Coates' in vitro was significantly influenced by the levels and ratio of cytokinin, sucrose and nitrogen salts in the MS medium (Wojtania et al, 2015).…”