Physiological responses of Raphidiopsis raciborskii (Cyanobacteria) strains to water conductivity: effect of sodium and magnesium ions

Lima, Daniel Vinícius Neves de; Goulart, Carolina L.; Azevedo, Sandra Maria Feliciano de Oliveira e

doi:10.1007/s10750-020-04265-3

Cited by 9 publications

(3 citation statements)

References 46 publications

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“…The required ranges of these values are defined in the relevant legal acts, and in the case of conductivity, it is usually <1000 µS·cm −1 . It was shown that a high conductivity (1500 µS·cm −1 ) had a neutral or positive effect on growth for the R. raciborskii strain [ 43 ]. In other papers, it was shown that the water conductivity affected the abundance of selected microorganisms in both freshwater and seawater [ 44 , 45 ].…”

Section: Resultsmentioning

confidence: 99%

Phytoremediation of CYN, MC-LR and ANTX-a from Water by the Submerged Macrophyte Lemna trisulca

Kucała

Saładyga

Kamiński

2021

Cells

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Cyanotoxins are harmful to aquatic and water-related organisms. In this study, Lemna trisulca was tested as a phytoremediation agent for three common cyanotoxins produced by bloom-forming cyanobacteria. Cocultivation of L. trisulca with Dolichospermum flos-aquae in BG11 medium caused a release of the intracellular pool of anatoxin-a into the medium and the adsorption of 92% of the toxin by the plant—after 14 days, the total amount of toxin decreased 3.17 times. Cocultivation with Raphidopsis raciborskii caused a 2.77-time reduction in the concentration of cylindrospermopsin (CYN) in comparison to the control (62% of the total pool of CYN was associated with the plant). The greatest toxin limitation was noted for cocultivation with Microcystis aeruginosa. After two weeks, the microcystin-LR (MC-LR) concentration decreased more than 310 times. The macrophyte also influenced the growth and development of cyanobacteria cells. Overall, 14 days of cocultivation reduced the biomass of D. flos-aquae, M. aeruginosa, and R. raciborskii by 8, 12, and 3 times, and chlorophyll a concentration in comparison to the control decreased by 17.5, 4.3, and 32.6 times, respectively. Additionally, the macrophyte stabilized the electrical conductivity (EC) and pH values of the water and affected the even uptake of cations and anions from the medium. The obtained results indicate the biotechnological potential of L. trisulca for limiting the development of harmful cyanobacterial blooms and their toxicity.

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Section: Resultsmentioning

confidence: 99%

Phytoremediation of CYN, MC-LR and ANTX-a from Water by the Submerged Macrophyte Lemna trisulca

Kucała

Saładyga

Kamiński

2021

Cells

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“…Hence, it has been proposed that these two genera are combined in the genus Raphidiopsis based on a phylogenetic study that used the 16S rRNA gene, 16S–23S ITS, and cpcBA sequences [ 18 ]. Consequently, a few studies have reported C. raciborskii as Raphidiopsis raciborskii [ 36 , 37 , 38 , 39 ]. This study also showed that these two genera were not separated in the phylogenetic tree of 16S rRNA, rpoC1 , and cpcBA genes ( Figure 1 , Figure 2 and Figure 5 ).…”

Section: Discussionmentioning

confidence: 99%

Development of Genus-Specific PCR Primers for Molecular Monitoring of Invasive Nostocalean Cyanobacteria

Kim

Park

2021

IJERPH

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The geographical range of invasive cyanobacteria with high toxigenic potential is widening because of eutrophication and global warming, thus, monitoring their appearance is necessary for safe water quality control. Most invasive cyanobacteria are nostocalean species, and their accurate identification by classical morphological methods may be problematic. In this study, we developed polymerase chain reaction (PCR) primers to selectively identify five invasive cyanobacterial genera, namely, Chrysosporum, Cuspidothrix, Cylindrospermopsis, Raphidiopsis, and Sphaerospermopsis, using genetic markers such as rbcLX, rpoB, rpoC1, and cpcBA, and determined the amplification conditions for each pair of primers. The primer performances were verified on single or mixed nostocalean cyanobacterial isolates. The five primers allowed selective identification of all the target genera. In field samples collected during summer, when cyanobacteria flourished in the Nakdong River, the respective PCR product was observed in all samples where the target genus was detected by microscopic analysis. Besides, weak bands corresponding to Sphaerospermopsis and Raphidiopsis were observed in some samples in which these genera were not detected by microscopy, suggesting that the cell densities were below the detection limit of the microscopic method used. Thus, the genus-specific primers developed in this study enable molecular monitoring to supplement the current microscopy-based monitoring.

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“…These findings indicate that low-temperature tolerance is not an intrinsic characteristic of R. raciborskii and that significant intraspecific heterogeneity exists for the temperature adaptability of this species. Similarly, the intraspecific heterogeneity of R. raciborskii has also been found in its response to light intensity [9,62,63] and conductivity [64], as well as its strategies for the utilization of N [26,27] and P [41]. In addition, varied growth rates, morphologies, and toxicities have been observed in genetically similar isolates of R. raciborskii [65,66].…”

Section: Phenotypic and Genetic Diversitymentioning

confidence: 92%

Recent Advances in the Ecology of Bloom-Forming Raphidiopsis (Cylindrospermopsis) raciborskii: Expansion in China, Intraspecific Heterogeneity and Critical Factors for Invasion

Liu

et al. 2023

IJERPH

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Water blooms caused by the invasive cyanobacterium Raphidiopsis raciborskii occur in many reservoirs in the tropical and subtropical regions of China. In recent decades, this species has spread rapidly to temperate regions. Phenotypic plasticity and climate warming are thought to promote the worldwide dispersion of R. raciborskii. However, investigations into the genetic and phenotypic diversities of this species have revealed significant intraspecific heterogeneity. In particular, competition between R. raciborskii and Microcystis aeruginosa was highly strain dependent. Although the concept of an ecotype was proposed to explain the heterogeneity of R. raciborskii strains with different geographic origins, microevolution is more reasonable for understanding the coexistence of different phenotypes and genotypes in the same environment. It has been suggested that intraspecific heterogeneity derived from microevolution is a strong driving force for the expansion of R. raciborskii. Additionally, temperature, nutrient fluctuations, and grazer disturbance are critical environmental factors that affect the population establishment of R. raciborskii in new environments. The present review provides new insights into the ecological mechanisms underlying the invasion of R. raciborskii in Chinese freshwater ecosystems.

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Physiological responses of Raphidiopsis raciborskii (Cyanobacteria) strains to water conductivity: effect of sodium and magnesium ions

Cited by 9 publications

References 46 publications

Phytoremediation of CYN, MC-LR and ANTX-a from Water by the Submerged Macrophyte Lemna trisulca

Phytoremediation of CYN, MC-LR and ANTX-a from Water by the Submerged Macrophyte Lemna trisulca

Development of Genus-Specific PCR Primers for Molecular Monitoring of Invasive Nostocalean Cyanobacteria

Recent Advances in the Ecology of Bloom-Forming Raphidiopsis (Cylindrospermopsis) raciborskii: Expansion in China, Intraspecific Heterogeneity and Critical Factors for Invasion

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