Physiological responses of transgenic tobacco plants expressing the dehydration-responsive RD22 gene of Vitis vinifera to salt stress

JAMOUSSI, Rahma JARDAK; El-Abbassi, M. M.; Jouira, Hatem Ben; Hanana, Mohsen; Zoghlami, Nèjia; Ghorbel, Abdelwahed; Mliki, Ahmed

doi:10.3906/bot-1301-53

Cited by 21 publications

(7 citation statements)

References 65 publications

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“…The DEG encoding glutathione S-transferase (GST) U17 (GSTU17) - like was continuously downregulated and found in Profile 0, which acts as a negative regulator of salt and drought stress response [41]. In addition, The DEGs encoding dehydration-responsive protein RD22 and Ca 2+ -dependent protein kinase 29 (CDPK29) were assigned to Profile 6 (Figure 6 and Table S10), which is involved in plant adaptation to drought and salinity stress [42,43].…”

Section: Resultsmentioning

confidence: 99%

Transcriptome Analysis Reveals New Insights into the Bacterial Wilt Resistance Mechanism Mediated by Silicon in Tomato

Jiang

Fan

Lin

et al. 2019

IJMS

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Bacterial wilt is a devastating disease of tomato caused by soilborne pathogenic bacterium Ralstonia solanacearum. Previous studies found that silicon (Si) can increase tomato resistance against R. solanacearum, but the exact molecular mechanism remains unclear. RNA sequencing (RNA-Seq) technology was used to investigate the dynamic changes of root transcriptome profiles between Si-treated (+Si) and untreated (−Si) tomato plants at 1, 3, and 7 days post-inoculation with R. solanacearum. The contents of salicylic acid (SA), ethylene (ET), and jasmonic acid (JA) and the activity of defense-related enzymes in roots of tomato in different treatments were also determined. The burst of ET production in roots was delayed, and SA and JA contents were altered in Si treatment. The transcriptional response to R. solanacearum infection of the +Si plants was quicker than that of the untreated plants. The expression levels of differentially-expressed genes involved in pathogen-associated molecular pattern-triggered immunity (PTI), oxidation resistance, and water-deficit stress tolerance were upregulated in the Si-treated plants. Multiple hormone-related genes were differentially expressed in the Si-treated plants. Si-mediated resistance involves mechanisms other than SA- and JA/ET-mediated stress responses. We propose that Si-mediated tomato resistance to R. solanacearum is associated with activated PTI-related responses and enhanced disease resistance and tolerance via several signaling pathways. Such pathways are mediated by multiple hormones (e.g., SA, JA, ET, and auxin), leading to diminished adverse effects (e.g., senescence, water-deficit, salinity and oxidative stress) normally caused by R. solanacearum infection. This finding will provide an important basis to further characterize the role of Si in enhancing plant resistance against biotic stress.

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Section: Resultsmentioning

confidence: 99%

Transcriptome Analysis Reveals New Insights into the Bacterial Wilt Resistance Mechanism Mediated by Silicon in Tomato

Jiang

Fan

Lin

et al. 2019

IJMS

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“…In a related study, similar increased chlorophyll retention was observed in transgenic plants expressing the stressresponsive gene VvRD22 with respect to the WT upon exposure to 400 mM NaCl (Jardak-Jamoussi et al, 2014).…”

Section: Response Of T1 Progenies To Salt Stressmentioning

confidence: 59%

Characterization of proteinase inhibitor-II gene under OsRGLP2promoter for salt stress in transgenic Nicotiana benthamiana

Rehman¹,

Jørgensen²,

Rasmussen³

et al. 2017

Turk J Biol

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Proteinase inhibitors in plants are mainly involved in conferring resistance against herbivores and pathogens. However, their role in abiotic stress resistance has also been reported. Therefore, transgenic tobacco plants were generated constitutively expressing the tomato proteinase inhibitor-II (PI-II) under control of the rice root germin-like protein 2 (OsRGLP2) promoter using Agrobacterium tumefaciens. T1 transgenic seedlings were subjected to stress treatments with different concentrations of sodium chloride (NaCl) (100 mM, 200 mM, 300 mM). Quantitative real-time PCR revealed considerable levels of expression and upregulation of PI-II in transgenic lines under salt stress. Considering the phenotypic effects, the transgenic seedlings showed better growth, higher chlorophyll content, higher proline contents, and higher average fresh weight when compared to the wild type. This comparative analysis of transgenic and wild-type seedlings allows us to suggest that PI-II gene activity can be upregulated via the OsRGLP2 promoter under osmotic stress conditions.

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“…Based on the hypothesis that a difference in gene regulation is an indicator of an adaptive response, many differentially-expressed genes under abiotic stress conditions were used to transform model or agronomically-interesting plants (Zhang and Blumwald, 2001 ;Figueras et al, 2004 ;Mukhopadhyay et al, 2004) in order to improve abiotic stress tolerance and productivity. In this context, a salt-induced VvRD22 gene from grapevine was recently characterized to be involved in salt stress tolerance in tobacco plants (Jardak-Jamoussi et al, 2014).…”

Section: Differential Expression Of Genes Under Salinitymentioning

confidence: 99%

Integration of omics and system biology approaches to study grapevine (Vitis vinifera L.) response to salt stress: a perspective for functional genomics - A review

et al. 2014

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The ability of plants to modify their behavior appropriately in response to salt stress is a major factor in their adaptation to this specific constraint. To date, environmental constraints, including salinity, become more and more unfavorable especially for glycophytes such as grapevines. Salt tolerance is a complex physiological and multigenic trait. Studying the functional networks of transcriptome, proteome and metabolome of grapevine plants subjected to salinity may help to identify candidate genes associated with salt tolerance mechanisms. Thus, the integration of omics tools (i.e., genomics, proteomics and metabolomics) with physiological approaches allows better understanding of the grapevine plant response and developing efficient markerassisted selection strategies in order to generate salt stress resistant grapevine varieties. In this review, research progress in grapevine responses to salt stress is discussed, highlighting the importance of the system biology approach for identifying molecular regulatory networks leading to a better adaptation ability of grapevine to salt stress.

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Physiological responses of transgenic tobacco plants expressing the dehydration-responsive RD22 gene of Vitis vinifera to salt stress

Cited by 21 publications

References 65 publications

Transcriptome Analysis Reveals New Insights into the Bacterial Wilt Resistance Mechanism Mediated by Silicon in Tomato

Transcriptome Analysis Reveals New Insights into the Bacterial Wilt Resistance Mechanism Mediated by Silicon in Tomato

Characterization of proteinase inhibitor-II gene under OsRGLP2promoter for salt stress in transgenic Nicotiana benthamiana

Integration of <em>omics</em> and system biology approaches to study grapevine (<em>Vitis vinifera</em> L.) response to salt stress: a perspective for functional genomics - A review

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